William H. Brondyk
University of Vermont
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Featured researches published by William H. Brondyk.
Molecular and Cellular Biology | 1995
William H. Brondyk; Colleen J. Mckiernan; Karen A. Fortner; Paul Stabila; Ronald W. Holz; Ian G. Macara
Rab3A is a small, Ras-like GTPase expressed in neuroendocrine cells, in which it is associated with secretory vesicle membranes and regulates exocytosis. Using the yeast two-hybrid system, we have identified a rat brain cDNA encoding a novel 50-kDa protein, which we have named Rabin3, that interacts with Rab3A and Rab3D but not with other small GTPases (Rab3C, Rab2, Ran, or Ras). Several independent point mutations in the effector domain of Rab3A (F51L, V55E, and G56D) which do not alter nucleotide binding by the GTPase abolish the interaction with Rabin3, while another mutation (V52A) appears to increase the interaction. These results demonstrate that the interaction is highly specific. However, a glutathione S-transferase-Rabin3 fusion protein associates only weakly in vitro with recombinant Rab3A and possesses no detectable GTPase-activating protein or nucleotide exchange activity, and Rabin3 overexpressed in adrenal chromaffin cells has no observable effect on secretion. The protein possess a sequence characteristic of coiled-coil domains and a second small region with sequence similarity to a Saccharomyces cerevisiae protein, Sec2p, Sec2p is essential for constitutive secretion in yeast cells and interacts with Sec4p, a close relative of the Rab3A GTPase. Rabin3 mRNA and protein are widely expressed but are particularly abundant in testes.
Journal of Biological Chemistry | 1994
Ronald W. Holz; William H. Brondyk; Ruth A. Senter; Luzette Kuizon; Ian G. Macara
Journal of Biological Chemistry | 1993
William H. Brondyk; C J McKiernan; E S Burstein; Ian G. Macara
Journal of Biological Chemistry | 1993
C J McKiernan; William H. Brondyk; Ian G. Macara
Journal of Biological Chemistry | 2003
Rene Lynn Schweickhardt; Xuliang Jiang; Louise M. Garone; William H. Brondyk
Archive | 2004
Louise M. Garone; Stephen Arkinstall; William H. Brondyk; Robert K. Campbell; Xuliang Jiang; Sean D. McKenna; Mark Tepper
Archive | 2004
William H. Brondyk; Xuliang Jiang; Rene Lynn Schweickhardt
Archive | 2002
William H. Brondyk; Xuliang Jiang; Rene Lynn Schweickhardt
Archive | 2004
Stephen Arkinstall; William H. Brondyk; Robert K. Campbell; Louise M. Garone; Xuliang Jiang; Sean D. McKenna; Mark Tepper
Archive | 2002
William H. Brondyk; Xuliang Jiang; Rene Lynn Schweickhardt