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Dive into the research topics where William H. Brondyk is active.

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Featured researches published by William H. Brondyk.


Molecular and Cellular Biology | 1995

Interaction cloning of Rabin3, a novel protein that associates with the Ras-like GTPase Rab3A.

William H. Brondyk; Colleen J. Mckiernan; Karen A. Fortner; Paul Stabila; Ronald W. Holz; Ian G. Macara

Rab3A is a small, Ras-like GTPase expressed in neuroendocrine cells, in which it is associated with secretory vesicle membranes and regulates exocytosis. Using the yeast two-hybrid system, we have identified a rat brain cDNA encoding a novel 50-kDa protein, which we have named Rabin3, that interacts with Rab3A and Rab3D but not with other small GTPases (Rab3C, Rab2, Ran, or Ras). Several independent point mutations in the effector domain of Rab3A (F51L, V55E, and G56D) which do not alter nucleotide binding by the GTPase abolish the interaction with Rabin3, while another mutation (V52A) appears to increase the interaction. These results demonstrate that the interaction is highly specific. However, a glutathione S-transferase-Rabin3 fusion protein associates only weakly in vitro with recombinant Rab3A and possesses no detectable GTPase-activating protein or nucleotide exchange activity, and Rabin3 overexpressed in adrenal chromaffin cells has no observable effect on secretion. The protein possess a sequence characteristic of coiled-coil domains and a second small region with sequence similarity to a Saccharomyces cerevisiae protein, Sec2p, Sec2p is essential for constitutive secretion in yeast cells and interacts with Sec4p, a close relative of the Rab3A GTPase. Rabin3 mRNA and protein are widely expressed but are particularly abundant in testes.


Journal of Biological Chemistry | 1994

Evidence for the Involvement of Rab3A in Ca2+-dependent Exocytosis from Adrenal Chromaffin Cells*

Ronald W. Holz; William H. Brondyk; Ruth A. Senter; Luzette Kuizon; Ian G. Macara


Journal of Biological Chemistry | 1993

Mutants of Rab3A analogous to oncogenic Ras mutants. Sensitivity to Rab3A-GTPase activating protein and Rab3A-guanine nucleotide releasing factor.

William H. Brondyk; C J McKiernan; E S Burstein; Ian G. Macara


Journal of Biological Chemistry | 1993

The Rab3A GTPase interacts with multiple factors through the same effector domain. Mutational analysis of cross-linking of Rab3A to a putative target protein.

C J McKiernan; William H. Brondyk; Ian G. Macara


Journal of Biological Chemistry | 2003

Structure-Expression Relationship of Tumor Necrosis Factor Receptor Mutants That Increase Expression

Rene Lynn Schweickhardt; Xuliang Jiang; Louise M. Garone; William H. Brondyk


Archive | 2004

FSH glycosylation mutant

Louise M. Garone; Stephen Arkinstall; William H. Brondyk; Robert K. Campbell; Xuliang Jiang; Sean D. McKenna; Mark Tepper


Archive | 2004

Methods of incerasing protein expession levels

William H. Brondyk; Xuliang Jiang; Rene Lynn Schweickhardt


Archive | 2002

Methods of increasing protein expression levels

William H. Brondyk; Xuliang Jiang; Rene Lynn Schweickhardt


Archive | 2004

Mutante de glicosilación de la fsh.

Stephen Arkinstall; William H. Brondyk; Robert K. Campbell; Louise M. Garone; Xuliang Jiang; Sean D. McKenna; Mark Tepper


Archive | 2002

Verfahren zur steigerung der expression von menschlichen tnf-rezeptor A method for enhancing the expression of human TNF receptor

William H. Brondyk; Xuliang Jiang; Rene Lynn Schweickhardt

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Robert K. Campbell

Marine Biological Laboratory

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