William L. Boyd

Blood Glucose and Lactic Acid in Relation to Milk Secretion.

Conclusions 1. There was no apparent relation between arterio-venous blood sugar differences and the following: period of time following milking, arterial blood sugar level and level of milk production. 2. Both glucose and lactic acid are absorbed by the lactating mammary gland in considerable quantities. While the glucose arterio-venous loss is not sufficient to account for all of the milk sugar, the combined loss of the two substances to the lactating mammary gland can account for all of the milk lactose.

Effect of carbohydrates on aspartic acid deaminase activity of bacteria.

Summary 1. The reduction in aspartic acid deaminase activity in cells harvested from a medium containing glucose has been shown not to be specific to this sugar, but rather is shared by other fermentable carbohydrates. 2. The effect of the carbohydrate varies among the several strains studied, and is not associated with the increase in hydrogen ion concentration due to the production of fermentation acids.

Thermophilic bacteria among arctic, subarctic, and alpine habitats.

Arctic, subarctic, and alpine regions support widely distributed populations of thermophilic bacteria among habitats where ambient temperatures vary from below lower psychrophilic to above upper thermophilic ranges. Although spore-forming bacilli are the most prevalent thermophiles, large numbers [290 to 1000 Colony Forming Units (CFUs) per g dry wt] of thermophilic actinomycetes were found in soils from parks in Reyjkavik, Iceland. Soils from arctic Greenland and alpine Colorado exhibited low numbers of thermophilic microorganisms (0 to 20 and 1 to 19 CFUs per g dry wt, respectively), but generally supported between 103and 106-times larger mesophile and psychrotroph populations. With two exceptions, lagoon sediments from arctic Alaska possessed larger numbers of thermophilic bacteria than the adjacent soils we sampled during 1955-1957. Cultivated soils from Troms0, Norway, showed greater thermophilic populations than nearby undisturbed soils (250 to 24,000 vs. 0 to 6700 CFUs per g dry wt); cultivation also increased numbers of mesophiles but not of psychrotrophs. Among 22 of 30 samples from geothermal areas of Iceland, thermophiles ranged from 1500 to 23,000 CFUs per g dry wt, and the ratios of thermophiles to mesophiles and psychrotrophs were much more compressed than from previously mentioned habitats. Thermophilic bacteria also were found in all but one of the volcanic and nongeothermal soils from Iceland (9 to 1500 and 22 to 19,000 CFUs per g dry wt, respectively), and a greater number of mesophiles and psychrotrophs were generally present in each case. Spore-forming, thermophilic bacteria make up part of the microbiota of polar, subpolar, and alpine ecosystems. Although geothermal habitats at temperature extremes may support the growth of stenothermophiles, and microhabitats exist with temperatures in the optimal range for the reproduction of eurithermophiles, until in situ methods are available for studying the specific contribution of thermophilic microorganisms to the metabolic activity of the total microbiota, their quantitative contribution to the ecology and economy of arctic, subarctic, and alpine habitats cannot be elucidated.

Distribution of thermophilic bacteria in arctic and subarctic habitats

Terrestrial and aquatic habitats of North American and the European Arctic and Subarctic were sampled for the presence of thermophilic, mesophilic, and psychrophilic bacteria. Thirteen geographic regions of Alaska, one in northern Canada, and seven in Norway were studied. In general, the number of thermophiles was low, and in many cases they were completely absent, particularly in samples of fresh water. They were found in highest numbers in materials which had been contaminated by human or animal sewage; in water, from hot springs and adjacent soils; in soils, particularly in Norway, which had been cultivated without the addition of organic manures; in soils which had been disturbed; and for unknown reasons they were present naturally in highest numbers in certain soils of northern Canada, where there was, in most cases, no known history of sewage contamination or cultivation. Although warmblooded animals may be the original source of some of these microorganisms, there is strong evidence to suggest that some strains occur naturally in waters and soils of these regions.

Effects of nutrition on aspartic acid deaminase system of Bacterium cadaveris.

Summary Data are presented to show that certain variations in nutrition, while not affecting the initial activity of an enzyme system, may influence markedly the stability of this system to successive washings.

A Study of Bovine Blood, Urine and Feces for the Presence of Bact. Abortus Bang.

Summary Bact abortus Bang Was not found often in the blood. urine and feces or cattle Known to be infected with this organism. Guinea pig inoculation proved to he a much more satisfactory method of isolation than cultural procedures.