William M. Stall

Confirmation and Control of a Paraquat-Tolerant Goosegrass (Eleusine indica) Biotype1

Diminished control of goosegrass was observed in tomato fields located in Manatee County, FL, after years of repeated paraquat use. Tolerance of the Manatee biotype to paraquat was confirmed by its comparison in greenhouse studies with a susceptible biotype from the Alachua County, FL. A 30-fold increase in paraquat rate was required to reach the 50% growth reduction level of the resistant biotype over the susceptible biotype. The Manatee biotype was not tolerant to clethodim, metribuzin, or sethoxydim. These herbicides provided adequate control of all the goosegrass biotypes tested. Nomenclature: Clethodim; metribuzin; paraquat; sethoxydim; goosegrass, Eleusine indica L. #3 ELEIN; tomato, Lycopersicou esculentum Mill. Additional index words: Additive, antagonism, herbicide resistance, synergism. Abbreviations: GR50, herbicide concentration required to inhibit goosegrass aboveground biomass by 50%; WAT, weeks after treatment.

Season-Long Interference of Yellow Nutsedge (Cyperus esculentus) with Polyethylene-Mulched Bell Pepper (Capsicum annuum)1

Yellow nutsedge, a weed commonly present in Florida vegetable fields, may substantially reduce crop yields when not controlled. Soil fumigation with methyl bromide effectively controls nutsedges, but methyl bromide is being phased out of production and use in the United States. Therefore, nutsedge management in bell pepper is a cause for concern. An experiment was conducted during four seasons (spring and fall of 1999 and 2000) to determine the tolerance of bell pepper grown at two in-row spacings (23 and 31 cm) to interference resulting from planted yellow nutsedge tuber densities (0 to 120 tubers/m2). Relative to yields with no nutsedge, pepper fruit yields in each season were reduced 10% with fewer than 5 planted tubers/m2. Yield losses increased more rapidly with an increase in initial nutsedge density from 0 to 30 than from 30 to 120 tubers/m2. With 30 nutsedge tubers/m2, large fruit yield was reduced 54 to 74% compared to that with no nutsedge. Nutsedge shoots overtopped the pepper plants as early as 6 wk after treatment when, with 15 planted tubers/m2, nutsedge interference reduced pepper plant biomass by 10 to 47%. In the absence of methyl bromide, weed control strategies with high efficacy against yellow nutsedge will be needed for bell pepper production. Nomenclature: Methyl bromide; yellow nutsedge, Cyperus esculentus L. #3 CYPES; bell pepper, Capsicum annuum L. ‘X3R Camelot’. Additional index words: Weed interference, yellow nutsedge competition, yield loss. Abbreviations: No., number; WAT, weeks after treatment.

Above- and belowground interference of purple and yellow nutsedge (Cyperus spp.) with tomato

Abstract Studies were conducted to determine the extent of full and partitioned interference of two nutsedge species with tomato. For full interference, the crop and the weed were transplanted in the same container. For belowground interference, tomato and either weed species were grown in the same container, but the canopies were separated. For aboveground interference, tomato and nutsedges were grown in separate containers placed adjacently, whereas for the no-interference treatment, tomato and nutsedge plants were grown in individual containers. Full interference by yellow nutsedge was more detrimental to tomato shoot dry weight accumulation (34% reduction) than was full interference by purple nutsedge (28% reduction). Belowground interference by purple nutsedge reduced tomato shoot dry weight (18%) more than did aboveground interference (9%). Yellow nutsedge interference above- or belowground reduced tomato shoot dry weight to a similar extent (19%). The belowground interference of both nutsedges with tomato resulted in deficient concentrations of nitrate in the sap of tomato (> 18% reduction). The growth of purple nutsedge was influenced more strongly by tomato shading than by belowground interference from the crop, whereas yellow nutsedge growth was equally affected by tomato above- and belowground. According to these results, shoot dry weight accumulation in tomato was affected to the same extent by belowground interference from purple and yellow nutsedge, and the higher effect of full interference by yellow nutsedge may be attributed to increased aboveground competition between tomato and yellow nutsedge. Nomenclature: Purple nutsedge, Cyperus rotundus L. CYPRO; yellow nutsedge, Cyperus esculentus L. CYPES; tomato, Lycopersicon esculentum Mill. ‘Solimar’.

Field Efficacy of Dactylaria higginsii as a Bioherbicide for the Control of Purple Nutsedge (Cyperus rotundus)1

Abstract: An isolate of the fungus Dactylaria higginsii obtained from purple nutsedge in Florida was highly pathogenic to Cyperus spp. The potential of this isolate as a bioherbicide was field tested in natural populations of purple nutsedge in Gainesville and Jay, FL. The fungus was applied in 0.5% Metamucil® as a carrier, and the treatments were: carrier only, 105 conidia/ml + carrier, and 106 conidia/ml + carrier. Treatments were applied as single, double, or triple postemergence (POST) sprays at biweekly intervals. The disease and secondary infections developed in about 5 and 15 d after inoculation, respectively, killing most of the infected leaves. All weed growth parameters and disease progress rates were affected by inoculum dosage and inoculation frequencies. Three inoculations, each at 106 conidia/ml, provided effective control of purple nutsedge compared to a single inoculation, as measured by shoot dry weight, tuber numbers, and tuber dry weight. Higher rates of disease progress and disease levels, defined by the area under the disease progress curve (AUDPC), occurred with three inoculations at 106 conidia/ml. Disease progress was slower and the level of weed control was lower at 105 conidia/ml compared to the higher inoculum level. Three applications of 106 conidia/ml provided >90% nutsedge control. Dactylaria higginsii appears to be an effective bioherbicide candidate deserving further development for commercial use. Nomenclature: Purple nutsedge, Cyperus rotundus L., #3 CYPRO, Dactylaria, D. higginsii (Luttrell) M. B. Ellis. Additional index words: Mycoherbicide, biological control of weeds, fungal pathogen. Abbreviations: AUDPC, area under the disease progress curve; PDA, potato dextrose agar; POST, postemergence; rG, disease progress.

Mechanisms of interference of smooth pigweed (Amaranthus hybridus) and common purslane (Portulaca oleracea) on lettuce as influenced by phosphorus fertility

Abstract Greenhouse studies were conducted to assess the intensity of smooth pigweed and common purslane aboveground interference (AI) and belowground interference (BI) with lettuce and to determine primary mechanisms of interference of each species as affected by P fertility rates. Lettuce was transplanted in mixtures with either smooth pigweed or common purslane according to four partitioning regimes: no interference, full interference, BI, and AI. Soil used was low in P for optimum lettuce yields, therefore P was added at rates of 0, 0.4, and 0.8 grams of P per liter of soil. Shoot and root biomass and plant height were measured for each species, as well as P tissue content. The data obtained indicated that smooth pigweed interfered with lettuce primarily through light interception by its taller canopy. A secondary mechanism of interference was the absorption of P from the soil through luxury consumption, increasing the P tissue content without enhancing smooth pigweed biomass accumulation. In contrast, common purslane competed aggressively with lettuce for P. Because the weed grew taller than lettuce, light interception was a secondary interference factor. Nomenclature: Common purslane, Portulaca oleracea L. POROL; smooth pigweed, Amaranthus hybridus L. AMACH; lettuce, Lactuca sativa L.

Influence of Nitrogen Fertilization on the Competitive Interactions of Cilantro (Coriandrum sativum) and Purple Nutsedge (Cyperus rotundas)

ABSTRACT The influence of nitrogen fertilization on the relative competitiveness of cilantro and purple nutsedge was determined using a single-density replacement series at 116 plants/m2. At the tested substitutive cilantro:purple nutsedge population proportions (100:0, 75:25, 50:50, 25:75 and 0:100) and four nitrogen application rates (0, 36, 72 and 108 kg/ha), the relative crowding coefficient indicated both species were equally aggressive at the 50:50 proportion after 40 days with 0 or 36 kg N/ha. As nitrogen was increased to 72 and 108 kg/ha, however, the competitiveness of purple nutsedge was enhanced (about 15 times more competitive than cilantro).

Phosphorus absorption in lettuce, smooth pigweed (Amaranthus hybridus), and common purslane (Portulaca oleracea) mixtures

Abstract Greenhouse studies were conducted to determine the influence of phosphorus (P) concentrations on the growth of lettuce, smooth pigweed, and common purslane in monocultures and in mixtures and to determine the P-absorption rate of each species over time. For the P-competition studies, lettuce–smooth pigweed and lettuce–common purslane mixtures were established in P-less hydroponic solutions. Each lettuce–weed mixture was established separately. Concentrations of P were 10, 20, 40, 80, and 160 mg L−1. Lettuce to weed planting proportions were 2:0, 0:2, and 1:1. In the mixtures, biomass of common purslane increased sharply between 10 and 20 mg P L−1, depressing lettuce growth. No biomass changes were observed in smooth pigweed as P concentration increased. However, both weeds increased their P content within this range, depriving lettuce of this nutrient. Common purslane competed for P for its own growth, whereas smooth pigweed absorbed P luxuriously. For the P-absorption studies, roots of lettuce, smooth pigweed, and common purslane plants were submersed in a 20 mg P L−1 solution for 1, 2.5, 5, 10, 20, 40, 60, 90, 180, 360, 720, and 1,440 min. Common purslane was shown to be the most aggressive species for the nutrient, absorbing 50% of the content in 295 min, whereas lettuce and smooth pigweed needed 766 and 825 min to absorb 10 mg P L−1. Nomenclature: Common purslane, Portulaca oleracea L. POROL; smooth pigweed, Amaranthus hybridus L. AMACH; lettuce, Lactuca sativa L.

Smooth Pigweed (Amaranthus hybridus L.) and Livid Amaranth (Amaranthus lividus) Interference with Cucumber (Cucumis sativus) 1

Field studies were conducted to determine the effect of season-long interference of smooth pigweed or livid amaranth on the shoot dry weight and fruit yield of cucumber. Smooth pigweed or livid amaranth densities as low as 1 to 2 weeds per m2 caused a 10% yield reduction in cucumber. The biological threshold of smooth pigweed or livid amaranth with cucumber is between 6 to 8 weeds per m2. Consequently, weed interference resulted in a reduction in cucumber fruit yield. Smooth pigweed, livid amaranth, and cucumber plant dry weight decreased as weed density increased. Evaluation of smooth pigweed, livid amaranth, and cucumber mean dry weights in interspecific competition studies indicated that cucumber reduced the dry weight of both species of amaranths. Nomenclature: Smooth pigweed, Amaranthus hybridus #3 AMACH; livid amaranth, Amaranthus lividus # AMALI; cucumber, Cucumis sativus L. Additional index words: Additive experiment, amaranth, cucumber, weed competition, yield loss.

Influence of method of phosphorus application on smooth pigweed (Amaranthus hybridus) and common purslane (Portulaca oleracea) interference in lettuce

Abstract Field trials were conducted to investigate the influence of P application method on the critical period of smooth pigweed and common purslane interference in lettuce. Studies were carried out in low-P histosols, where supplemental P fertilization is needed for lettuce production. Phosphorus was either broadcast or banded 5 cm beneath the lettuce rows at rates of 250 or 125 kg ha−1, respectively. Seedlings of either smooth pigweed or common purslane were transplanted at a density of 16 plants per 5.4 m2 (6-m row by 0.9 m wide). Weed interference duration was achieved by manual removal 2, 4, 6, or 8 wk after lettuce emergence and subsequently keeping the plot weed free until harvest. A weed-free control within each P regimen was also established. Marketable head number, head fresh yield, and head diameter were measured at harvest. Weed-free lettuce fresh yield was 20% higher with banded P than broadcast applications. In the weed–lettuce mixtures, the P regimen by weed removal interaction affected lettuce fresh yield and head diameter but not head number. Compared with broadcast P application, banded P extended the time needed to cause significant weed interference in lettuce by 10 d: from 24 to 34 d for smooth pigweed and from 37 to 47 d for common purslane. Nomenclature: Common purslane, Portulaca oleracea L. POROL; smooth pigweed, Amaranthus hybridus L. AMACH; lettuce, Lactuca sativa L.

Critical Period of Interference between American Black Nightshade and Triploid Watermelon

Abstract Field trials were conducted in the spring of 2007 and 2008 to investigate the critical period of interference between American black nightshade and triploid watermelon. To determine the critical period, the maximum period of competition and minimum weed-free period were examined. American black nightshade (2 plants m−2) was established into watermelon plots at watermelon transplanting and removed at 0, 1, 2, 3, 4, and 5 wk after transplanting to determine the maximum period of competition. American black nightshade (2 plants m−2) was established into watermelon plots at 0, 1, 2, 3, 4, and 5 wk after transplanting and remained until watermelon harvest to determine the minimum weed-free period. To avoid yield loss from exceeding 10% of a crop grown weed-free, the maximum period of competition and minimum weed-free period were found to be 3.9 and 3.6 weeks after transplanting, respectively. Therefore, if American black nightshade is controlled at any time during the critical period of 3.6 to 3.9 wk after transplanting, yield loss should not exceed 10% of a crop grown weed-free. Nomenclature: American black nightshade, Solanum americanum Mill. SOLAM; watermelon, Citrullus lanatus (Thunb.) Matsumura and Nakai cv. ‘Super Crisp’.