Wirt W. Smith

HEMATOLOGIC PROBLEMS ASSOCIATED WITH THE USE OF EXTRACORPOREAL CIRCULATION FOR CARDIOVASCULAR SURGERY

Excerpt Extracorporeal circulation maintained by an artificial heart-lung apparatus is now an essential and established procedure for certain types of cardiovascular surgery. With this new developm...

Effects of in vivo Hyperoxia on Erythrocytes 1. Hemolysis in Mice Exposed to Hyperbaric Oxygenation.

Summary In vivo lytic sensitivity of mouse erythrocytes to hyperbaric oxygenation paralleled their in vitro sensitivity to H2O2. In vitro lysis was associated with lipid peroxide formation. The in vivo and in vitro lysis and in vitro lipid peroxide formation were enhanced by vit. E deficiency and reduced by treatment of animals with a tocopherol acetate. It was suggested that hyperoxic hemolysis in vivo was a direct or indirect result of abnormal lipid peroxidation.

The effects of normal cerebrospinal fluid on blood clotting and fibroblast growth.

Summary In 50 normal CSF samples, no thrombin, fibinogen, prothrombin, proconvertin, proaccelerin, antihemophilic globulin or thromboplastin activity was found. These samples had no effects different from those produced by artificial control solutions on the coagulation time of a thrombin-fibrinogen system, and they demonsrated no fibrinolytic activity. In addition, they had no effect on chick fibroblast growth. There is no idication from these results that CSF specifically interferes with the healing of ruptured intracranial aneurysms.

The Relation between Storage Time of Erythrocytes and Their Percentage Viability after Transfusion

During storage of blood the percentage of viable erythrocytes decreases at such a rate that when these percentages are plotted against time of storage, they fall on an S‐shaped curve. This curve can be explained on the assumption that the resistance of red cells to in vitro aging is normally distributed, i.e., follows the normal probability curve. By a mathematical transformation used in other cases of biological standardization, the curve can be changed to a straight line, the slope of which, if accurately determined with a sufficient number of recipients, is the most accurate method of estimating the rate of in vitro aging.