Wojciech Dziewiszek

Experimental application of xenogenous antlerogenic cells in replacement of auricular cartilage in rabbits

Abstract:  Background:  Regenerative medicine in the recent years aims at explaining involvement of stem cells in regenerative processes and applying this knowledge in fulfilling human needs to find new, more efficient therapeutic methods. Growing antlers constitute a model organ for examining regeneration processes of tissues because they are the only mammalian appendages capable of regeneration. The rate of growth of deer antlers makes them one of the quickest growing structures in mammals. The cells taking part in this process have a considerable proliferating potential. The aim of the study was to analyze the possibility of using xenogenous antlerogenic cells (AC) in regeneration of cartilaginous tissues in non‐immunosuppressed animals.

Application of Cornelian Cherry Iridoid-Polyphenolic Fraction and Loganic Acid to Reduce Intraocular Pressure

One of the most common diseases of old age in modern societies is glaucoma. It is strongly connected with increased intraocular pressure (IOP) and could permanently damage vision in the affected eye. As there are only a limited number of chemical compounds that can decrease IOP as well as blood flow in eye vessels, the up-to-date investigation of new molecules is important. The chemical composition of the dried Cornelian cherry (Cornus mas L.) polar, iridoid-polyphenol-rich fraction was investigated. Loganic acid (50%) and pelargonidin-3-galactoside (7%) were found as the main components. Among the other constituents, iridoid compound cornuside and the anthocyans cyanidin 3-O-galactoside, cyanidin 3-O-robinobioside, and pelargonidin 3-O-robinobioside were quantified in the fraction. In an animal model (New Zealand rabbits), the influence of loganic acid and the polyphenolic fraction isolated from Cornelian cherry fruit was investigated. We found a strong IOP-hypotensive effect for a 0.7% solution of loganic acid, which could be compared with the widely ophthalmologically used timolol. About a 25% decrease in IOP was observed within the first 3 hours of use.

Characteristics of Antlerogenic Stem Cells and Their Potential Application

Marek Cegielski1,4, Ilona Iżykowska1, Wojciech Dziewiszek2,4, Maciej Zatoński3, Marek Bochnia3,4 and Olga Kalisiak5 1Departments of Histology and Embryology, Medical University of Wroclaw 2 Departments of Pharmacology, Medical University of Wroclaw, 3Department of Otolaryngology, Faculty of Dentistry, Medical University of Wroclaw, 4Stem Cells spin Ltd. Wrocław Technology Park, Wroclaw, 5Department of Clinical Sciences, Faculty of Veterinary Medicine, Warsaw University of life Sciences SGGW2 Poland Corresponding author Marek Cegielski, VD, PhD Medical University of Wroclaw, Dept. of Histology and Embryology ul. Chalubinskiego 6a 50-368 Wroclaw, Poland marcel@hist.am.wroc.pl

Changes of electric cochlea activity of guinea pigs during argon laser stapedotomy

Small electric signals appear on surface of a cochlea when the ear is stimulated by sound. A level of the signals can be measured of the electric activity of cochlea. The aim of the experiments was recording of changes of the cochlear potentials during argon laser stapedotomy. On the base of the recording the limits of the safe argon laser stapedotomy have been preliminary estimated. The series of argon laser pulses lasting 0.2-0.5 s and of 16 s interval between the pulses are preferable for safety of argon laser stapedotomy. The pulse peak power should be below 1 W.

Preliminary report on the influence of an argon laser on electrophysiology of cochlea in guinea pigs

A small electrical potential generated by cochlear, when the ear is stimulated by sound, has been used to estimate the influence of the argon laser on cochlear activity in guinea pigs. The detected potentials were recorded prior to, during and after laser irradiation of bone of cochlear, Laser pulses of 0.5-1 s duration and peak power of 100-660 mW were used in the experiment. The number of pulses in the series and the time interval between single laser pulses were varied, too. Depending on the laser irradiation parameters the values of CM returned or no to the pre-impact value.

Analysis of Biological Acoustic Waves by Means of the Phase–Sensitivity Technique

The analysis of hearing mechanisms and research on the influence of various internal (pathologies, ageing) and external (trauma, vibration, noise) factors on sound perception are usually done using acoustic waves induced in the external ear canal. Stimuli which have been used for this purpose are: clicks, tone bursts, half-sine-waves, single tones or pairs of tones. The Corti organ’s responses to the external stimuli have either an electric or acoustic character. In the former case, these are cochlear microphonics (CMs) picked up from the surface or from the inside of the cochlea, which are usually used as an indicator of damage to the organ of Corti in animals. In the latter case, these are acoustic waves that appear in the external ear canal as a result of stimulation. The acoustic waves have an important clinical value. Taking into account the presence of nonlinear distortions in the cochlea, the waves that appear after stimulation with a pair of tones are called distortion product otoacoustic emissions (DPOAE). In studies on CMs, the origin of stimulating waves is often a single earphone (controlled by a generator of defined, often periodical, electrical signals) placed in the external auditory canal. In studies on DPOAE, a probe with two miniature earphones and one microphone is placed in the external auditory canal. The earphones are controlled by two generators of frequencies f1 and f2 and the microphone converts the returned DPOAE wave with a combination frequency, e.g. 3 1 2 2 f f f = − , into an electrical signal.