Wolfgang Nimmich

Structure of the capsular polysaccharide from the Klebsiella K8 reference strain 1015

The structure of the capsular polysaccharide from the Klebsiella K8 reference strain 1015 has been elucidated. The structure was deduced from sugar analysis, different methylation analyses, a uronic acid degradation, and NMR spectroscopy. It is concluded that the polysaccharide is composed of pentasaccharide repeating units with the structure: [formula: see text] The structure differs from that of the previously published structure of the capsular polysaccharide from Klebsiella K8, which originates from another strain and has the following structure: [formula: see text] The serological similarity between the two strains is most likely derived from a common tetrasaccharide which is substituted in different ways in the two strains. Since the strain in the present investigation originates from the Klebsiella K reference strain collection of the International Escherichia and Klebsiella Centre, Copenhagen, Denmark, it is suggested that it should keep the designation K8. The other polysaccharide with Klebsiella K8 specificity should be renamed as K8,52,59 based on the cross-reactivity of the strain (I. Orskov, unpublished).

Structural studies of the Klebsiella o group 9 lipopolysaccharide

Abstract The structure of the O-specific side-chains in the Klebsiella O group 9 lipopolysaccharide has been investigated. Methylation analysis, alone and in combination with partial hydrolysis by acid and trideuteriomethylation, were the main methods used. Partial hydrolysis of the fully methylated lipopolysaccharide, followed by reduction, trideuteriomethylation, and characterisation of a disaccharide derivative, gave further information. O -Acetyl groups were located by methylation analysis of chemically modified lipopolysaccharides. As a result of these studies, it is concluded that the side chains are composed of pentasaccharide repeating units, and a structure for these units is proposed.

Structural studies of the capsular polysaccharide of Klebsiella type 33.

The capsular polysaccharide from Klebsiella type 28 has been studied by methylation analysis, a modified Smith-degradation procedure, and uronic acid degradation with subsequent oxidation and elimination of the substituents of the oxidized residue. The polysaccharide contained the hexasaccharide repeating-unit shown below. The terminal D-glucopyranose residue was hydrolysed by emulsin, indicating a beta linkage. The anomeric natures of other glycosidic linkages were determined by characterization of fragments obtained during the degradative studies. The D-galactopyranose residue was not present in any fragment, but is assumed to be alpha-linked from optical-rotation considerations. (see article)

Structural studies of the capsular polysaccharide of Klebsiella type 52

Abstract The structure of the capsular polysaccharide from Klebsiella Type 52 has been investigated. Methylation analysis, characterization by gas-liquid chromatography-mass spectrometry of oligosaccharide derivatives obtained on partial hydrolysis of the methylated polysaccharide with acid, and specific degradation of the methylated polysaccharide by successive treatments with base and acid followed by characterization of the product, were the principal methods used. The polysaccharide is composed of hexasaccharide repeating-units containing D -glucuronic acid, D -galactose, and L -rhamnose, in the ratios 1:3:2. A structure for these units, disregarding the anomeric natures of the sugar residues, is proposed.

Structural studies of the capsular polysaccharide of Klebsiella type 59

The structure of the capsular polysaccharide from Klebsiella type 59 has been investigated by methylation analysis, a modified Smith-degradation procedure, and uronic acid degradation followed by oxidation and elimination of the substituents of the oxidized residue. The oligomeric fragments produced by these degradative procedures were isolated and characterized. O-Acetyl groups were identified and their position determined. The polysaccharide consists of the following pentasaccharide repeating-unit (dotted lines indicate that only some of the residues carry the O-acetyl substituent). See article.

Structural studies of the capsular polysaccharide of Klebsiella type 28

Abstract The capsular polysaccharide from Klebsiella type 28 has been studied by methylation analysis, a modified Smith-degradation procedure, and uronic acid degradation with subsequent oxidation and elimination of the substituents of the oxidized residue. The polysaccharide contained the hexasaccharide repeating-unit shown below. The terminal D -glucopyranose residue was hydrolysed by emulsin, indicating a β linkage. The anomeric natures of other glycosidic linkages were determined by characterization of fragments obtained during the degradative studies. The D -galactopyranose residue was not present in any fragment, but is assumed to be α-linked from optical-rotation considerations.

The Klebsiella type 38 capsular polysaccharide: identification of 3-deoxy-L-glycero-pentulosonic acid and structural studies

Abstract D -Glucose and D -galactose (ratio 1:1) comprise ≈80% of the capsular polysaccharide from Klebsiella type 38, and the acidic component has been identified as 3-deoxy- L - glycero -pentulosonic acid. The polysaccharide is composed of pentasaccharide repeating units, for which a structure is proposed on the basis of methylation analysis, and Smith-degradation and partial hydrolysis studies.

Structural studies of the capsular polysaccharide of Klebsiella type 81

The structure of the capsular polysaccharide from Klebsiella Type 81 has beeninvestigated. Methlylation analysis, uronic acid degradation, Smith degradation, andgraded acid hydrolysis were the principle methods used. Theanomeric nature of theglycosidic linkages was determined by characterization of fragments obtained from thevarious methods of degradation used. One of the L-rhamnosidic linkages was notpresent in any of these fragments, but is assumed to be an alpha-linkage from consideerationsof optical-rotation data. These studies show that they polysaccharide consits of thefollowing hexasaccharide repeating-unit: yield2)-alpha-L-Rhap-(1yields3)-alpha-L-Rhap-(1-yields4)-beta-D-GlcAP-(1-yields2)-alpha-L-Rhap-(1-yields3)-alpha-L-Rhap-(1-yields3)-beta-D-Galp-(1-yields.

Structural Studies of the Klebsiella Type 57 Capsular Polysaccharide.

The structure of the capsular polysaccharide from Klebsiella type 57 has been investigated. Methylation analysis, uronic acid degradation, modified Smith degradation and graded acid hydrolysis were the principle methods used. Pure oligomeric fragments were isolated using the three methods of degradation and characterized by chemical and physical methods. These studies show the structure to consist of a tetrasaccharide repeating unit (all sugar residues have the D-configuration and are pyranosidic).

Structural studies of the capsular polysaccharide of Klebsiella type 37.

The structure of the Klebsiella type 37 capsular polysaccharide has been investigated. Methylation analysis, various specific degradations, and n.m.r. spectroscopy were the principal methods used. It is concluded that the polysaccharide is composed of tetrasaccharide repeating-units having the structure 4-O-Lac-d-GlcA  4-O-[(S)-1-carboxyethyl]-d-glucuronic acid: