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Featured researches published by Wookeun Bae.


Biodegradation | 2001

Optimal operational factors for nitrite accumulation in batch reactors

Wookeun Bae; Seungcheon Baek; Jinwook Chung; Yong-Woo Lee

The environmental factors that affected the accumulation of nitrite in nitrifying reactors were investigated using a mixed culture. A batch reactor with 50 mg-N/l of ammonia was used. The pH, temperature and dissolved oxygen concentration were varied. The concentration of unionized free ammonia also changed with the oxidation of ammonia and the variation of pH and temperature. The accumulation of nitrite was affected sensitively by pH and temperature. A higher nitrite concentration was observed at pH 8-9 or temperature around 30 °C. The dissolved oxygen also affected, giving the highest nitrite accumulation at around 1.5 mg/l. These were the favoredconditions for nitrite production. The free ammonia concentration influenced thenitrite accumulation also, by inhibiting nitrite oxidation. The inhibition becameapparent at a concentration of approximately 4 mg/l or above, but insignificant atbelow 1 mg/l. Thus, simultaneously high free ammonia concentration and maximumspecific ammonia-oxidation rate (above 15 × 10-3 mg-N/mg-VSSċh)were needed for a significant nitrite accumulation. When the two conditions were met, thenthe highest accumulation was observed when the ratio of the maximum specific oxidationrate of ammonia to the maximum specific oxidation rate of nitrite (ka/kn) was highest.Under the optimal operating conditions of pH 8, 30 °C and 1.5 mg/l of dissolvedoxygen, as much as 77% of the removed ammonia accumulated in nitrite.


Environmental Science & Technology | 2010

Operational boundaries for nitrite accumulation in nitrification based on minimum/maximum substrate concentrations that include effects of oxygen limitation, pH, and free ammonia and free nitrous acid inhibition.

Seongjun Park; Wookeun Bae; Bruce E. Rittmann

Recent studies on shortcut biological nitrogen removal (SBNR), which use the concept of denitrification from nitrite, have reported the key factors affecting nitrite build-up, such as dissolved oxygen (DO) limitation, pH, and free ammonia (FA) and free nitrous acid (FNA) inhibition. This study extends the concept of the traditional minimum substrate concentration (S(min)) to explain the simultaneous effect of those factors. Thus, we introduce the minimum DO concentration (DO(min)) and the maximum substrate concentration (S(max)) that are needed to support a steady-state biological system. We define all three values as the MSC values. The model provides a method to identify good combinations of pH, DO, and total ammonium nitrogen (TAN) to support shortcut nitritation. We use MSC curves to show that the effect of DO-alone and the effect of DO plus direct pH inhibition cannot give strong enough selection against nitrite oxidizing bacteria to work in a practical setting. However, adding the FA and FNA effects gives a strong selection effect that is accentuated near pH 8. Thus, a generalized conclusion is that having pH approximately 8 is favorable in many situations. We defined a specific operational boundary to achieve shortcut nitritation coupled to anaerobic ammonium oxidation (ANAMMOX), in which the effluent concentrations of total nitrite and total ammonium should be approximately equal. Experimental results for alkaline and acidic nitrite-accumulating systems match the trends from the MSC approach. In particular, acidic systems had to maintain higher total ammonium, total nitrite, and DO concentrations. The MSC values are a practical tool to define the operational boundaries for selecting ammonium-oxidizing bacteria while suppressing nitrite-oxidizing bacteria.


Biodegradation | 2006

Factors affecting nitrous oxide production: a comparison of biological nitrogen removal processes with partial and complete nitrification.

Sun-Jin Hwang; Kwangun Jang; Hyun-Sup Jang; JiHyeon Song; Wookeun Bae

Nitrous oxide (N2O) emission from biological nitrogen removal (BNR) processes has recently received more research attention. In this study, two lab-scale BNR systems were used to investigate the effects of various operating parameters including the carbon to nitrogen (C/N) ratio, ammonia loading, and the hydraulic retention time on N2O production. The first system was operated in a conventional BNR mode known as the Ludzack–Ettinger (LE) process, consisting of complete denitrification and nitrification reactors, while the second one was operated in a shortcut BNR (SBNR) mode employing partial nitrification and shortcut denitrification, which requires less oxygen and carbon sources. As the C/N ratio was decreased, a significant increase in N2O production was observed only in the anoxic reactor of the LE process, indicating that N2O was released as an intermediate of the denitrification reaction under the carbon-limited condition. However, the SBNR process did not produce significant N2O even at the lowest C/N ratio of 0.5. When the SBNR process was subjected to increasing concentrations of ammonia, N2O production from the aerobic reactor was rapidly increased. Furthermore, the increasing production of N2O was observed mostly in the aerobic reactor of the SBNR process with a decline in hydraulic retention time. These experimental findings indicated that the increase in N2O production was closely related to the accumulation of free ammonia, which was caused by an abrupt increase of the ammonium loading. Consequently, the partial nitrification was more susceptible to shock loading conditions, resulting in a high production of N2O, although the SBNR process was more efficient with respect to nitrogen removals as well as carbon and oxygen requirements.


Biodegradation | 2002

Nitrite reduction by a mixed culture under conditions relevant to shortcut biological nitrogen removal.

Jinwook Chung; Wookeun Bae

Dissimilative reduction of nitrite by nitrite-acclimated cellswas investigated in a batch reactor under various environmental conditions that can beencountered in shortcut biological nitrogen removal (SBNR: ammonia to nitrite andnitrite to nitrogen gas). The maximum specific nitrite reduction rate was as much as 4.3 times faster than the rate of nitrate reduction when individually tested, but the reaction was inhibited in the presence of nitrate when the initial nitrate concentration was greater than approximately 25 mg-N/l or the initialNO3-N/NO2-N ratio was larger than 0.5. Nitrite reduction was also inhibited by nitrite itself when theconcentration was higher than that to which the cells had been acclimated. Therefore, it was desirable to avoid excessively high nitrite and nitrate concentrations in a denitrification reactor. Nitrite reduction, however, was not affected by an alkaline pH (in the range of 7–9) or a high concentration of FA (in the range of 16–39 mg/l), which can be common in SBNR processes. The chemical oxygen demand (COD) requirement for nitrite reduction was approximately 22–38% lower than that for nitrate reduction, demonstrating that the SBNR process can be economical. The specific consumption,measured as the ratio of COD consumed to nitrogen removed, was affected by the availability of COD and the physiological state of the cells. The ratio increased when the cells grew rapidly and were storing carbon and electrons.


Dyes and Pigments | 2003

Photodegradation of acid red 114 dissolved using a photo-Fenton process with TiO2

Jong-Min Lee; Moon-Sun Kim; Byungho Hwang; Wookeun Bae; Byung-Woo Kim

Abstract A dye acid red 114 (C.I. 23635) was photochemically removed by adding ferric ion (Fe3+), TiO2 particles, and H2O2 in the presence of the UV radiation. The removal rates of the acid red 114 (C.I. 23635) dye were 0.183, 0.210, and 0.233 mg/l/min when the concentrations of Fe3+ were 50, 100, and 130 mg/l. The removal rate of the dye increased from 0.173, through 0.200, 0.210 to 0.260 mg/l/min when the H2O2 concentrations were 10, 50, 100, and 150 mg/l, respectively. The removal rates were 0.200, 0.207, 0.210, 0.273, and 0.293 mg/l/min when the concentrations of TiO2 were 40, 60, 100, 500, and 1000 mg/l, respectively. The removal rate at pH 2.5 was higher than any other; pH 3.5, 5.5, and 8.5 and the photodegradation efficiency increased with the flow rate of air in the range from 1 to 10 l/min. From these results, relationships between the removal rate and the concentration of added Fe3+, H2O2, and TiO2 could be expressed as the following second-order equations, respectively, Rremoval=3×10−6 ×(CFe3+)2+5×10−5 ×(CFe3+)+0.1729; Rremoval=2×10−6 ×(CH2O2)2+2×10−3 ×(CH2O2)+0.1759; and Rremoval=−1×10−7 ×(CTiO2)2+2×10−4×(CTiO2)+0.1867.


International Journal of Systematic and Evolutionary Microbiology | 2008

Oceanobacillus caeni sp. nov., isolated from a Bacillus-dominated wastewater treatment system in Korea

Ji-Hyun Nam; Wookeun Bae; Dong-Hun Lee

A Gram-positive, rod-shaped, spore-forming bacterium, strain S-11T, was isolated from the activated sludge of a Bacillus-dominated wastewater treatment system in South Korea and was characterized using a polyphasic approach in order to determine its taxonomic position. Cells (0.5-0.6 x 2.0-2.2 microm) were motile by means of a single subpolar flagellum. They bore ellipsoidal endospores that lay in a central position in swollen sporangia. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain S-11T was a member of the genus Oceanobacillus. 16S rRNA gene sequence similarity values and DNA-DNA relatedness of strain S-11T to the type strains of other Oceanobacillus species were less than 96.2 and 66.0 %, respectively. Strain S-11T showed distinct differences in the G+C content of the genomic DNA (33.6 mol%). The major cellular fatty acids were iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0. The major isoprenoid quinone was MK-7. There were also some physiological differences in comparison with the type strains of Oceanobacillus species: tests for production of acetoin and acid production from dulcitol, erythritol, myo-inositol and sorbitol were positive. The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain S-11T from the six Oceanobacillus species and subspecies with validly published names. Strain S-11T therefore represents a novel species, for which the name Oceanobacillus caeni sp. nov. is proposed, with the type strain S-11T (=KCTC 13061T =CCUG 53534T =CIP 109363T).


Journal of Hazardous Materials | 2009

Effects of operational conditions on sludge degradation and organic acids formation in low-critical wet air oxidation.

Jinwook Chung; Mikyung Lee; Jaehwan Ahn; Wookeun Bae; Yong-Woo Lee; Hojae Shim

Wet air oxidation processes are to treat highly concentrated organic compounds including refractory materials, sludge, and night soil, and usually operated at supercritical water conditions of high temperature and pressure. In this study, the effects of operational conditions including temperature, pressure, and oxidant dose on sludge degradation and conversion into subsequent intermediates such as organic acids were investigated at low critical wet oxidation conditions. The reaction time and temperature in the wet air oxidation process was shown an important factor affecting the liquefaction of volatile solids, with more significant effect on the thermal hydrolysis reaction rather than the oxidation reaction. The degradation efficiency of sludge and the formation of organic acids were improved with longer reaction time and higher reaction temperature. For the sludge reduction and the organic acids formation under the wet air oxidation, the optimal conditions for reaction temperature, time, pressure, and oxidant dose were shown approximately 240 degrees C, 30min, 60atm, and 2.0L/min, respectively.


Environmental Technology | 2005

Comparison of influence of free ammonia and dissolved oxygen on nitrite accumulation between suspended and attached cells.

Jinwook Chung; Hojae Shim; Yong-Woo Lee; Wookeun Bae

The shortcut biological nitrogen removal (SBNR) hybrid (suspended cells combined with attached cells) process is an innovative technology that nitrosofies ammonium to nitrite and then denitrifies nitrite to nitrogen gas. Theoretically, this results in a 25% savings of the oxygen needed for nitrification and a 40% of savings in carbon source needed for denitrification. In this study, the influences of free ammonia (FA) and dissolved oxygen (DO) concentrations on nitrite accumulation were investigated to find the optimal operational factors for stable nitrite accumulation over a long period. The maximum specific utilization rates for ammonium (qa) and nitrite (qn) were determined for suspended and attached cells taken from a bench-scale SBNR reactor and a pilot-scale livestock wastewater treatment plant reactor. For the ammonium and nitrite oxidations in both reactors, the attached cells were more resistant to the FA concentration, but were more significantly influenced by the DO concentration than the suspended cells. In addition, the effect of the DO concentration was more significant than that of the FA concentration for both types of cells from both reactors. In this SBNR hybrid system, a simultaneous manipulation of DO concentration (<1.5 mg l-1) and FA concentration (10-20 mg l-1) was required for maintaining high levels of nitrite accumulation.


Journal of Hazardous Materials | 2009

Recovery of nitric acid from waste etching solution using solvent extraction.

Chang-Hoon Shin; J. Kim; Jun-Young Kim; Hyun-Sang Kim; Hyang-Sook Lee; Debasish Mohapatra; Jae-Woo Ahn; Jong-Gwan Ahn; Wookeun Bae

A process was developed to recover nitric acid from the waste stream of wafer industry using solvent extraction technique. Tributyl phosphate (TBP) was selected among several extractants because of its better selectivity towards HNO(3), overall superiority in operation, favorable physical properties and economics. The waste solution containing 260 g/L CH(3)COOH, 460 g/L HNO(3), 113 g/L HF and 19.6g/L Si was used as feed solution for process optimization. In the pre-treatment stage >99% silicon and hydrofluoric acid was precipitated out as Na(2)SiF(6). Equilibrium conditions for HNO(3) recovery were optimized from the batch test results as: four stages of extraction at an organic:aqueous (O:A) ratio of 3, four stages of scrubbing at O:A ratio of 5 and five stages of stripping at an O:A ratio of 1.5. The extraction of HNO(3) was suppressed by the presence of acetic acid (HAc) in the feed solution. To examine the feasibility of the extraction system a continuous operation was carried out for 200 h using a multistage mixer-settler. The concentration of pure HNO(3) recovered was 235 g/L with a purity of 99.8%.


Materials Science and Engineering: C | 2016

Aminated polyethersulfone-silver nanoparticles (AgNPs-APES) composite membranes with controlled silver ion release for antibacterial and water treatment applications.

M. Salman Haider; Godlisten N. Shao; S.M. Imran; Sung Soo Park; Nadir Abbas; M. Suleman Tahir; Manwar Hussain; Wookeun Bae; Hee Taik Kim

The present study reports the antibacterial disinfection properties of a series of silver nanoparticle (AgNP) immobilized membranes. Initially, polyethersulfone (PES) was functionalized through the introduction of amino groups to form aminated polyethersulfone (NH2-PES, APES). AgNPs were then coordinately immobilized on the surface of the APES composite membrane to form AgNPs-APES. The properties of the obtained membrane were examined by FT-IR, XPS, XRD, TGA, ICP-OES and SEM-EDAX analyses. These structural characterizations revealed that AgNPs ranging from 5 to 40 nm were immobilized on the surface of the polymer membrane. Antibacterial tests of the samples showed that the AgNPs-APES exhibited higher activity than the AgNPs-PES un-functionalized membrane. Generally, the AgNPs-APES 1 cm × 3 cm strip revealed a four times longer life than the un-functionalized AgNPs polymer membranes. The evaluation of the Ag(+) leaching properties of the obtained samples indicated that approximately 30% of the AgNPs could be retained, even after 12 days of operation. Further analysis indicated that silver ion release can be sustained for approximately 25 days. The present study provides a systematic and novel approach to synthesize water treatment membranes with controlled and improved silver (Ag(+)) release to enhance the lifetime of the membranes.

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