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Featured researches published by Xianchang Tian.


Mitochondrial DNA | 2015

The complete mitochondrial genome of natural Misgurnus bipartitus (Cypriniformes: Cobitidae)

Songqian Huang; Xianchang Tian; Weimin Wang; Wen Song; Xiujie Zhang; Xiaohui Bai; Xiaojuan Cao

Abstract Misgurnus bipartitus, a small sized freshwater fish species, is one of the most important cultured fish in East Asia. In this study, the complete mitochondrial genome of M. bipartitus is sequenced to be 16,636 bp in length, including 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs, a control region and the origin of the light strand replication. The overall base composition of M. bipartitus in descending order is A 29.79 %, T 27.95%, C 25.90% and G 16.36%, with a slight A + T bias. The mitogenome sequence data may provide useful information to the population genetics analysis of M. bipartitus and the elucidation of evolutionary mechanisms in Cobitidae.


PLOS ONE | 2016

High-Throughput Sequencing Identifies MicroRNAs from Posterior Intestine of Loach (Misgurnus anguillicaudatus) and Their Response to Intestinal Air-Breathing Inhibition

Songqian Huang; Xiaojuan Cao; Xianchang Tian; Weimin Wang

MicroRNAs (miRNAs) exert important roles in animal growth, immunity, and development, and regulate gene expression at the post-transcriptional level. Knowledges about the diversities of miRNAs and their roles in accessory air-breathing organs (ABOs) of fish remain unknown. In this work, we used high-throughput sequencing to identify known and novel miRNAs from the posterior intestine, an important ABO, in loach (Misgurnus anguillicaudatus) under normal and intestinal air-breathing inhibited conditions. A total of 204 known and 84 novel miRNAs were identified, while 47 miRNAs were differentially expressed between the two small RNA libraries (i.e. between the normal and intestinal air-breathing inhibited group). Potential miRNA target genes were predicted by combining our transcriptome data of the posterior intestine of the loach under the same conditions, and then annotated using COG, GO, KEGG, Swissprot and Nr databases. The regulatory networks of miRNAs and their target genes were analyzed. The abundances of nine known miRNAs were validated by qRT-PCR. The relative expression profiles of six known miRNAs and their eight corresponding target genes, and two novel potential miRNAs were also detected. Histological characteristics of the posterior intestines in both normal and air-breathing inhibited group were further analyzed. This study contributes to our understanding on the functions and molecular regulatory mechanisms of miRNAs in accessory air-breathing organs of fish.


Cytogenetic and Genome Research | 2016

Production of Tetraploid Gynogenetic Loach Using Diploid Eggs of Natural Tetraploid Loach, Misgurnus anguillicaudatus, Fertilized with UV-Irradiated Sperm of Megalobrama amblycephala without Treatments for Chromosome Doubling

Songqian Huang; Xiaojuan Cao; Xianchang Tian; Weiwei Luo; Weiming Wang

The gynogenesis phenomenon in nature mainly appears in the reproduction of fish and invertebrates. So far, gynogenesis has been successfully induced in many fish species with the aid of some physical or chemical methods for chromosome doubling. However, few fish can produce gynogenetic progenies, genetically identical or similar to the somatic cells of the mothers, without a treatment for the doubling of chromosomes, which may be related to apomixis, premeiotic endoreduplication, or premeiotic endomitosis. At present, no studies are available about fish with normal ovarian structures producing gynogenetic progenies that could spontaneously double their chromosomes. According to the analyses of flow cytometry, chromosome number, and microsatellites, we found that, with the use of UV-irradiated sperm of blunt snout bream Megalobrama amblycephala, tetraploid loach Misgurnus anguillicaudatus produced tetraploid gynogenetic progenies without any treatments for the doubling of chromosomes. To determine the genetic relationships of gynogenetic progenies and their maternal parent, microsatellite genotyping was conducted. The results indicated that the reason for spontaneous chromosome duplication in gynogenetic progenies may be cytokinesis or inhibition of the extrusion of the second polar body. This is the first report on fish with normal ovarian structures that can produce gynogenetic progenies which spontaneously double their chromosomes and which are genetically identical or similar to the somatic cells of the mothers.


Mitochondrial DNA | 2016

The complete mitochondrial genome of natural Cobitis elongatoides (Cypriniformes: Cobitidae).

Songqian Huang; Tea Tomljanović; Xianchang Tian; Yizhou Wang; Xiaojuan Cao

Abstract Cobitis elongatoides is a small sized freshwater fish species that is widely distributed in Europe, especially in Croatia. In this study, the complete mitochondrial genome of C. elongatoides is sequenced to be 16,540 bp in length, including 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs, a control region and the origin of the light strand replication. The overall base composition of C. elongatoides in descending order is A 29.37%, T 28.53%, C 25.34%, and G 16.76%, with a slight A + T bias. The mitogenome sequence data may provide useful information to the population genetics analysis of C. elongatoides and the elucidation of evolutionary mechanisms in Cobitidae.


Marine Biotechnology | 2016

Transcriptomic Analysis of Compromise Between Air-Breathing and Nutrient Uptake of Posterior Intestine in Loach (Misgurnus anguillicaudatus), an Air-Breathing Fish

Songqian Huang; Xiaojuan Cao; Xianchang Tian


Biochemical Systematics and Ecology | 2015

Genetic diversity and parentage assignment in Dojo loach, Misgurnus anguillicaudatus based on microsatellite markers

Xiaohui Bai; Songqian Huang; Xianchang Tian; Xiaojuan Cao; Gangming Chen; Weimin Wang


Aquaculture Research | 2017

Ploidy and growth performance of hybrid progeny between tetraploid Misgurnus anguillicaudatus and Paramisgurnus dabryanus

Songqian Huang; Xiaojuan Cao; Xianchang Tian; Weimin Wang


한국수산과학회 양식분과 학술대회 | 2015

TETRAPLOIDIZATION AND DIPLOIDIZATION OF WILD LOACH Misgurnus anguillicaudatus IN CHINA, REVEALED BY COLD SHOCK AND GYNOGENESIS

Songqian Huang; Xianchang Tian; Xiaojuan Cao; Wang Wei-min


한국수산과학회 양식분과 학술대회 | 2015

TRANSCRIPTOME PROFILE ANALYSES OF OVARIAN TISSUES FROM DIPLOID AND TETRAPLOI LOACHES Misgurnus anguillicaudatus AND DEVELOPMENT OF SSR MARKERS FOR THE LOACHES WITH DIFFERENT PLOIDIES

Weiwei Luo; Xianchang Tian; Xiaojuan Cao


한국수산과학회 양식분과 학술대회 | 2015

ABERRANT MEIOSES IN HYBRID PROGENY PRODUCED BY NATURAL TETRAPLOID LOACH Misgurnus anguillicaudatus (♀) AND NATURAL Paramisgurnus dabryanus (♂)

Xiaojuan Cao; Xianchang Tian; Songqian Huang; Xiu-wen Xu; Weimin Wang

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Xiaojuan Cao

Huazhong Agricultural University

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Songqian Huang

Huazhong Agricultural University

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Weimin Wang

Huazhong Agricultural University

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Weiwei Luo

Huazhong Agricultural University

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Xiaohui Bai

Huazhong Agricultural University

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Gangming Chen

Huazhong Agricultural University

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Wen Song

Huazhong Agricultural University

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Xiujie Zhang

Huazhong Agricultural University

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Yizhou Wang

Huazhong Agricultural University

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