Xiang-Zhao Guo

Characterization of the complete mitochondrial genome of Oxygymnocypris stewartii (Cypriniformes: Cyprinidae).

Abstract The complete mitochondrial genome of Oxygymnocypris stewartii is 16,646 bp in size, containing 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and 2 non-coding regions (the control region and the origin of light-strand replication). The gene order of O. stewartii mitogenome is similar to those observed in most other vertebrates. The complete mitogenome sequence information of O. stewartii can provide useful data for further studies on phylogenetics, stock evaluation and conservation genetics.

Isolation and characterization of twenty-four polymorphic microsatellite loci in Oxygymnocypris stewartii

Twenty-four polymorphic microsatellite loci were isolated from Oxygymnocypris stewartii and were characterized in 42 individuals collected from the Yarlung Tsangpo River in Tibet, China. The number of alleles per locus ranged from 4 to 24 with an average of 12.542. The expected heterozygosity and Shannon-Wiener diversity index ranged from 0.731 to 0.932 and from 1.342 to 2.887, respectively. These polymorphic loci will be available for the evaluation of genetic diversity and population genetic structure of O. stewartii.

The complete mitogenome sequence of Ptychobarbus dipogon (Cypriniformes: Cyprinidae)

Abstract The complete mitochondrial genome of Ptychobarbus dipogon is 16,787 bp in length, consisting of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and 2 non-coding regions: control region (CR) and origin of light-strand replication (OL). The gene order of P. dipogon mitogenome is similar to those observed in most other vertebrates. The complete mitogenome sequence of P. dipogon can provide useful data for further studies on population structure, phylogenetics and conservation genetics of this species.

Isolation and characterization of nineteen novel polymorphic microsatellite loci for the northern snakehead Channa argus

Channa argus is one of the most important economic fish species in China. However, there is limited genetic information on its population structure and genetic diversity. Nineteen novel polymorphic microsatellite loci were isolated and characterized in 42 individuals from one natural population collected from the Poyang Lake in Jiangxi Province. The number of alleles per locus ranged from 5 to 18 with an average of 11.16. The observed and expected heterozygosities ranged from 0.214 to 1.000 and from 0.630 to 0.923, respectively. The average polymorphic information content value was 0.814. Among these polymorphic microsatellites, eleven loci conformed to Hardy–Weinberg equilibrium. These microsatellite loci are currently being used to assess the genetic diversity and population structure of C. argus and will contribute to the effective conservation and rational utilization of genetic resources of this species.

Isolation and characterization of eighteen polymorphic microsatellite loci in Schizopygopsis younghusbandi Regan and cross-amplification in three other schizothoracinae species

Eighteen polymorphic microsatellite loci were isolated from Schizopygopsis younghusbandi Regan and the characterization of these loci was assessed in 46 individuals collected from the Yarlung Tsangpo River in Tibet, China. The number of alleles per locus ranged from 2 to 14. The expected heterozygosity and Shannon-Wiener diversity index ranged from 0.022 to 0.879 and from 0.059 to 2.313, respectively. The cross-species amplification and applicability of these loci were tested in three other Schizothoracinae species belonging to Schizothorax and Oxygymnocypris. These loci will be useful for the evaluation of genetic diversity and population genetic structure in S. younghusbandi and other related species.

Development and characterization of 20 polymorphic microsatellite loci for the Lhasa schizothoracin Schizothorax waltoni

Schizothorax waltoni is a valued economic fish endemic to Tibet, China. However, there is little information on genetic population structure and genetic diversity for this species. Twenty polymorphic microsatellite loci were isolated and characterized in 42 individuals collected from the Yarlung Tsangpo River in Tibet. The number of alleles per locus ranged from 9 to 28 with an average of 18.45. The expected heterozygosity and Shannon-Wiener diversity index ranged from 0.716 to 0.942 and from 1.675 to 3.038, respectively. These microsatellite loci are currently being used to evaluate the genetic diversity and genetic population structure to contribute to conservation and management of S. waltoni.

Genetic diversity and population structure of the northern snakehead (Channa argus Channidae: Teleostei) in central China: implications for conservation and management

Major threats to freshwater fish diversity now include loss of native genetic diversity as a consequence of translocations of fishes between sites and from hatcheries to sites, and small effective population sizes resulting from overfishing and/or habitat loss. Ten polymorphic microsatellite markers were employed to evaluate genetic diversity, population genetic structure and gene flow amongst nine populations of the ecologically and economically important fish, the northern snakehead (Channa argus), in three river systems in central China. Multiple analyses revealed evidence of high genetic diversity and pronounced subdivision based on both regional separation and on river systems. A lack of evidence of genetic bottleneck over recent generations was consistent with the long-term stability of population size and contemporary distribution. The effective population sizes for most C. argus populations were small, suggesting the need for future conservation efforts focusing on these populations. Different lines of evidence point to the local enhancement of stocks by both aquaculture-reared fish and the transfer of wild fish. This study illustrates how human activities may affect genetic diversity and population genetic structure of C. argus populations, and highlights the need for new management regimes to protect native freshwater fish genetic diversity.

Isolation and characterization of twenty-one polymorphic microsatellite loci from Schizothorax o'connori and cross-species amplification.

1Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of China, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China 2Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, People’s Republic of China 3Centre for Marine Environmental and Economic Research, School of Biological Sciences, Victoria University of Wellington, P. O. Box 600, Wellington 6140, New Zealand

Isolation and characterization of twenty-three polymorphic microsatellite loci in Schizothorax macropogon

Twenty-three polymorphic microsatellite loci were isolated from Schizothorax macropogon and were characterized in 42 individuals collected from the Yarlung Tsangpo River in Tibet, China. The number of alleles per locus ranged from 5 to 32 with an average of 20.17. The expected heterozygosity and Shannon-Wiener diversity index ranged from 0.709 to 0.951 and from 1.421 to 3.183, respectively. These microsatellite loci will be valuable for the evaluation of genetic diversity and population genetic structure of S. macropogon.