Xianglan Jiang
Xuzhou Medical College
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Publication
Featured researches published by Xianglan Jiang.
Talanta | 2011
Dongzhi Yang; Yi-qiang An; Xianglan Jiang; Daoquan Tang; Yuanyuan Gao; Hong-Tao Zhao; Xiao-wen Wu
A novel method combining high performance liquid chromatography (HPLC) fingerprint and simultaneous quantitative analysis of multiple active components was developed and validated for quality evaluation of one type of traditional Chinese medicine preparations: Shuang-huang-lian (SHL) oral liquid formulation. For fingerprint analysis, 45 peaks were selected as the common peaks to evaluate the similarities among several different SHL oral liquid preparations collected from manufacturers. Additionally, simultaneous quantification of eleven markers, including chlorogenic acid, caffeic acid, rutin, forsythiaside, scutellarin, baicalin, forsythin, luteoloside, apigenin, baicalein and wogonin, was performed. Statistical analysis of the obtained data demonstrated that our method has achieved desired linearity, precision and accuracy. Finally, concentrations of these eleven markers in SHL oral liquid prepared by different manufacturers in China were determined. These results demonstrated that the combination of HPLC chromatographic fingerprint and simultaneous quantification of multi-ingredients offers an efficient and reliable approach for quality evaluation of SHL oral liquid preparations.
Phytotherapy Research | 2011
Daoquan Tang; Yaqin Wei; Yuanyuan Gao; Xiaoxing Yin; Dongzhi Yang; Jie Mou; Xianglan Jiang
The protective effect of rutin on the glomerulosclerosis of diabetic nephropathy (DN) in rat mesangial cells was investigated. The cultured mesangial cells were divided into eight groups: normal, solvent control, high glucose, low dose of rutin, moderate dose of rutin, high dose of rutin, captopril and Ginkgo biloba extract. The cell cycles, type IV collagen and laminin in cytoplasm, TGF‐β1 mRNA of mesangial cells, Smad 2/3 and Smad 7, and the activities of four antioxidant indexes including T‐SOD, MDA, CAT and GSH‐Px were measured by flow cytometry, radioimmunoassay, RT‐PCR, western blotting and visible spectrophotometry, respectively. Compared with the high glucose group, rutin decreased the cell percentages of the G0/G1 phase and inhibited the expression of Smad 2/3, laminin and type IV collagen, and TGF‐β1 mRNA level, significantly. The antioxidant capacity, the cell percentages of S phase and Smad 7 expression were significantly increased by rutin. These results suggest that rutin is a potent protective agent against glomerulosclerosis in DN. Copyright
Journal of Separation Science | 2014
Daoquan Tang; Zheng Li; Xianglan Jiang; Yin-jie Li; Qian Du; Dongzhi Yang
A rapid and sensitive ultra high performance liquid chromatography method with diode array detection was developed for the fingerprint analysis and simultaneous determination of seven active compounds in Xiaoyanlidan (XYLD) tablets. The chromatographic separations were obtained on an Agilent Eclipse plus C18 column (50 × 2.1 mm id, 1.8 μm) using gradient elution with water/formic acid (1%) and acetonitrile at a flow rate of 0.4 mL/min. Within 63 min, 36 peaks could be selected as the common peaks for fingerprint analysis to evaluate the similarities among several samples of XYLD tablets collected from different manufacturers. In quantitative analysis, seven compounds showed good regression (R > 0.9990) within test ranges and the recovery of the method was within the range of 95.9-104.3%. The method was successfully applied to the simultaneous determination of seven compounds in six batches of XYLD tablets. These results demonstrate that the combination of chromatographic fingerprint analysis and simultaneous multi-ingredient quantification using the ultra high performance liquid chromatography method with diode array detection offers a rapid, efficient, and reliable approach for quality evaluation of XYLD tablets.
Biomedical Chromatography | 2015
Jing-ying Qiu; Xu Chen; Xiao-xiao Zheng; Xianglan Jiang; Dongzhi Yang; Yanyan Yu; Qian Du; Dao-quan Tang; Xiaoxing Yin
A rapid and useful approach for screening potential bioactive components in Ginkgo biloba extract (GBE) with preventive effect against diabetic nephropathy (DN) was developed using mesangial cells extraction coupled with high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis. Mesangial cells were first divided into two groups according to their treatments with high glucose or high glucose plus GBE. After incubation for 4, 8, 12, 16, 24 and 48 h, the cells were harvested and extracted with 40% acetic acid in water before LC-MS/MS analysis. Then, 19 compounds and five metabolites were found to selectively combine with mesangial cells. Notably, compounds including quercetin and rutin were identified or tentatively characterized according to the results of retention time and MS spectra, which is highly consistent with our previous reports that quercetin and rutin are potent protective agents against glomerulosclerosis in DN. Therefore, all these results indicate that target cell extraction coupled with LC-MS/MS analysis can be successfully applied for predicting the bioactive components in GBE with preventive effect against DN.
Analytical and Bioanalytical Chemistry | 2010
Daoquan Tang; Dongzhi Yang; Anbang Tang; Yuanyuan Gao; Xianglan Jiang; Jie Mou; Xiaoxing Yin
Chromatographia | 2012
Dongzhi Yang; Shian Sun; Chonggang Fu; Xianglan Jiang; Daoquan Tang
Latin American Journal of Pharmacy | 2011
Ziming Zhao; Xianglan Jiang; Xiaoxin Yin; Daoquan Tang; Yi-qiang An; Xu Chen; Jing Wu
Latin American Journal of Pharmacy | 2011
Dongzhi Yang; Yuanyuan Gao; Xianglan Jiang; Weifeng Dong; Daoquan Tang
Latin American Journal of Pharmacy | 2011
Xiao Liu; Dongzhi Yang; Jing Wu; Daoquan Tang; Xiaoxin Yin; Yuanyuan Gao; Xianglan Jiang
Latin American Journal of Pharmacy | 2011
Xianglan Jiang; Jing Wu; Daoquan Tang; Yuanyuan Gao; Dongzhi Yang; Ziming Zhao; Hui Chen