Xiaogang Lin

Research on the interaction between tubeimoside 1 and HepG2 cells using the microscopic imaging and fluorescent spectra method.

The treatment of cancer draws interest from researchers worldwide. Of the different extracts from traditional Chinese medicines, Tubeimoside 1 (TBMS 1) is regarded as an effective treatment for cancer. To determine the mechanism of TBMS 1, the shape/pattern of HepG2 cells based on the microscopic imaging technology was determined to analyze experimental results; then the fluorescent spectra method was designed to investigate whether TBMS 1 affected HepG2 cells. A three-dimensional (3D) fluorescent spectra sweep was performed to determine the characteristic wave peak of HepG2 cells. A 2D fluorescent spectra method was then used to show the florescence change in HepG2 cells following treatment with TBMS 1. Finally, flow cytometry was employed to analyze the cell cycle of HepG2 cells. It was shown that TBMS 1 accelerated the death of HepG2 cells and had a strong dose- and time-dependent growth inhibitory effect on HepG2 cells, especially at the G2/M phase. These results indicate that the fluorescent spectra method is a promising substitute for flow cytometry as it is rapid and cost-effective in HepG2 cells.

UV light activated NO2 gas sensing based on Au nanoparticles decorated few-layer MoS2 thin film at room temperature

Nitrogen dioxide (NO2) plays a key role in environmental protection and human health. Recently, molybdenum disulfide (MoS2) representative of novel 2D materials has been utilized to detect NO2 gas, still hindered by the weak response, high operation temperature, as well as poor recovery characteristics. Herein, we report a UV light-assisted, room-temperature, recoverable, sensitive and selective NO2 gas sensing based on few-layer MoS2 nanosheet-Au nanoparticle composites serving as the sensing layer. In the dark condition, the as-prepared MoS2-Au sensor showed a response of 10% toward 2.5 ppm NO2 more than two times larger than MoS2 one, arising from more reaction sites (spillover effect and produced interfaces) and smaller baseline resistance after Au incorporation. Undesirably, all sensors exhibited an incomplete recovery. When MoS2-Au sensors were exposed to NO2 gas under UV illumination, better performance in terms of three-time enhanced response, full recovery and favorable repeatability was achieved in comparison with that in the dark case. Two aspects were responsible for these phenomena. On one hand, additional photoinduced charge carriers ensured sufficient gas-solid interactions between sensing layer and target molecules, thereby resulting in a larger response. The other aspect lay in effective separation of these charge carriers at MoS2/Au interfaces, contributing to recoverable and repeatable reactions. MoS2-Au composites were adopted for NO2 detection under UV illumination and exhibited a promising capacity of UV photodetector as well as an inspiring room-temperature gas detection, which provided an alternative strategy to design a single optoelectronic device with multi-functions.

Research of the interaction between kangai injection and human serum albumin by fluorescence spectroscopy

The interaction between drugs and serum albumin is the theoretical basis of pharmacology research. Kangai injection with invigorating Qi, enhancing the immune function, is widely used for a variety of malignant tumor treatment. Fluorescence spectroscopy was adopted due to its high sensitivity and other advantages. The interaction between kangai injection and human serum albumin (HSA) in physiological buffer (pH 7.4) was investigated by fluorescence spectroscopy and UV-Vis absorption spectroscopy. The results of fluorescence spectrum at three temperature (296K, 303K and 310K) showed the degree of binding at 310K is the highest. Also, the maximum emission peak has a slight blue shift, which indicates that the interaction between kangai injection and HSA has an effect on the conformation of HSA. That is, the microenvironment of tryptophan increase hydrophobic due to the increase of the concentration of kangai injection. Results obtained from analysis of fluorescence spectrum and fluorescence intensity indicated that kangai injection has a strong ability to quench the intrinsic fluorescence of HSA. And according to the Stern-Volume equation, the quenching mechanism is static quenching, which is further proved by the UV-Vis absorption spectroscopy.

The numerical simulation and goniometric measurements of cells light scattering based on Mie theory

Optical diagnostic technique, due to its rapid and non-invasive for the diagnosis diseases at the cellular level, can be performed in vivo and allow for real-time diagnosis. While light scattering method is capable of characterizing the structural properties of tissue at the cellular and subcellular scale. In this paper, the spherical models of cells light scattering were established based on Mie, and the distribution curves of scattering intensity in the range of 0~180 degrees were got to explore change rule of cells light scattering information at the molecular level. Also, a platform for experiments used to measure the light scattering information of cells was built to get the change rule of cells light scattering information in wide angular range. And the particle size distribution (PSD) of cells was got by the inversion algorithm. A comparative analysis between numerical simulation and goniometric measurements revealed that the forward-scattering and side-scattering were influenced by the particle size of cells and relative index of refraction between cells and surrounding media. It could also be concluded that it was necessary to get and analyze the light scattering information of larger scattering angle range, which may be related to the intracellular organelles and nucleus.

Cell light scattering characteristic research based on FDTD algorithm

As with the number of cancer increases year by year, so it is important to be found and treated earlier. With biological cells and tissues are sensitive to infrared and visible light, cell morphology and physical structure of the optical properties can easily obtain, we can provide theoretical basis for the early diagnosis of cancer by observing the difference of optical properties between normal and cancerous cells. Compared with Mie scattering theory, finite difference time domain (FDTD) algorithm can analyze any complex structure model. In this paper we use mathematical modeling method to establish the single cell mathematical model and with finite difference time domain algorithm to simulate the propagation and scattering of light in the biological cells, you can calculate the scattering of electromagnetic field distribution at anytime and anywhere. With radar cross section (RCS) to measure the results of the scattering characteristics. Due to the difference between normal cells and cancerous cells are embodied in cell shape, size and the refractive index, through the simulation we can get different cell parameters of light scattering information, Find out the cell parameters change the changing rule of the influence on the scattering characteristics and find out change regularity of scattering characteristics. These data can judge very accurate of the cells is normal or cancerous cells.

Detecting devices in dynamic, module, and time sharing

In the research and development of multi-parameter precision detecting system, various parameters need to be measured (such as length, diameter, surface roughness and so on). We not only measure some parameters statically but also measure others dynamically and calculate others (such as volume, density) using some detected parameters. At the same time, this system need higher precision and higher measuring speed. We propose a new detecting idea for this system--detecting devices in dynamic, module and time-sharing. And we design and optimize multi-parameter high precision measurement system employing the method. This idea includes three parts: the first part is dynamical part. We can make system more stability and more continuity in high detecting speed. The second part is module part. We can settle on a solution to measure similar parameters and make system structure more reasonable and reduce error factors. The third is time-sharing part. We solve the problem how to allot time to every parameter and make every measurement part and its software tie in. The detecting idea has been employed to design and optimize several multi-parameter precision detecting systems. Now these systems are running successfully in workshop.