Xiaokang Wu
Xi'an Jiaotong University
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Featured researches published by Xiaokang Wu.
Current Microbiology | 2010
Xiaokang Wu; Chaofeng Ma; Lei Han; Muhammad Nawaz; Fei Gao; Xuyan Zhang; Pengbo Yu; Chang’an Zhao; Lianchuan Li; Aiping Zhou; Juan Wang; John E. Moore; B. Cherie Millar; Jiru Xu
The investigation provides molecular analyses of the faecal microbiota in type 2 diabetic patients. In order to characterise the gut microbiota in diabetic patients and to assess whether there are changes in the diversity and similarity of gut microbiota in diabetic patients when compared with healthy individuals, bacterial DNAs from 16 type 2 diabetic patients and 12 healthy individuals were extracted from faecal samples and characterised by PCR-denaturing gradient gel electrophoresis (DGGE) with primers specifically targeting V3 region of the 16S rRNA gene, as well as been sequenced for excised gel bands. The counts of Bacteroides vulgatus, Clostridium leptum subgroup and Bifidobacterium genus were assessed using quantitative PCR. By comparing species diversity profiles of two groups, we observed that there were no significant differences between diabetic and healthy group, although a few diabetic individuals (D6, D8) exhibited a remarkable decrease in species profiles. As for the similarity index, it was lower in inter-group than that in intra-group, which showed that the composition of gut microbiota in diabetic group might be changed due to diabetes status. Sequencing results also revealed that bacterial composition of diabetic group was different from that of the healthy group. B. vulgatus and Bifidobacterium genus were low represented in the microbiota of diabetic group, and the significant decrease was observed for Bifidobacterium by real-time PCR. Taken together, in this work we observed the characterisation of gut microbiota in diabetic patients, which suggestes that the gut microbiota of diabetes patients have some changes associated with occurrence and development of diabetes.
Current Microbiology | 2011
Chaofeng Ma; Xiaokang Wu; Muhammad Nawaz; Jinsong Li; Pengbo Yu; John E. Moore; Jiru Xu
The study provides molecular analyses of fecal microbiota of diarrhea patients infected with four different types of viruses. Fecal specimens from 52 patients with viral diarrhea (13 each of adenovirus, norovirus, rotavirus, and astrovirus) and six healthy individuals were collected and etiological viral agent was confirmed by enzyme immunoassay and specific PCR. To assess the changes in microbial diversity in patients with viral diarrhea, DNA from stool were extracted and characterized by PCR-denaturing gradient gel electrophoresis (DGGE) with universal primers specific for the V3 region of 16S rRNA gene. The strongest bands of the DGGE profiling were excised and sequenced to identify the dominant groups. Bacteroides vulgatus, Bifidobacterium, and Lactobacillus genera were also enumerated by real time PCR. The results revealed that bacterial diversity and similarity in feces from viral diarrhea groups were significantly lower (mean H′/
Microbiological Research | 2014
Wei Li; Lei Han; Pengbo Yu; Chaofeng Ma; Xiaokang Wu; Jiru Xu
Microbial Ecology | 2014
Wei Li; Lei Han; Pengbo Yu; Chaofeng Ma; Xiaokang Wu; John E. Moore; Jiru Xu
H_{ \max }^{\prime }
Biomedicine & Pharmacotherapy | 2017
Hafiz Muhammad Ishaq; Imran Shair Mohammad; Hui Guo; Muhammad Shahzad; Yin Jian Hou; Chaofeng Ma; Zahid Naseem; Xiaokang Wu; Peijie Shi; Jiru Xu
Canadian Journal of Microbiology | 2013
Lei Han; Jine Lei; Shaoshan Han; Li He; Chaofeng Ma; Pengbo Yu; Xiaokang Wu; Jiru Xu
0.89–0.94, 29–43, respectively) as compared with those of healthy individuals (mean H′/
Current Microbiology | 2011
Muhammad Nawaz; Juan Wang; Aiping Zhou; Chaofeng Ma; Xiaokang Wu; John E. Moore; B. Cherie Millar; Jiru Xu
Journal of Medical Virology | 2017
Chaofeng Ma; Xiaoyun Hao; Huiling Deng; Rui Wu; Jifeng Liu; Yang Yang; Shen Li; Yifei Han; Mingmin Wei; Junjun Zhang; Jingjun Wang; Hengxin Li; Huaiyu Tian; Bing Xu; Pengbo Yu; Xiaokang Wu
H_{ \max }^{\prime }
Journal of Ayub Medical College Abbottabad | 2017
Hafiz Muhammad Ishaq; Muhammad Shahzad; Xiaokang Wu; Chaofeng Ma; Jiru Xu
Pakistan Journal of Zoology | 2018
Hafiz Muhammad Ishaq; Muhammad Shahzad; Xiaokang Wu; Chaofeng Ma; Jiru Xu
1.36, 59, respectively). Sequencing of dominant bands affirmed that diarrhea groups were mainly comprised of phylum Firmicutes, such as genera Enterococcus, Peptostreptococcaceae incertae sedi, Streptococcus, Weissella, and Clostridium, and opportunistically pathogenic genus Shigella, while dominant group in healthy individuals was phylum Bacteroidetes. Copy number of Bacteroides vulgatus, Bifidobacterium, and Lactobacillus genera was also reduced significantly in viral diarrhea groups as compared to healthy group. It is concluded that opportunistic pathogens increases, while other species of commensal microbiota decrease significantly in the viral diarrhea patients and dysbacteriosis is dependent on type of virus infection.