Xiaoyan Pan

Splenic densities measurements and its relationship with the extent of pathologic changes

In this study, we measured the density of spleen by an integrated volume-density meter and correlated with their pathology changes. The densities of spleen from 134 autopsy cases were measured with different extent of pathologic changes by the meter. All samples were divided into 2 groups: intact and pathologic groups. The extent of pathologic changes was classified into 3 stages: fibrosis, slight edema, severe edema according to the histological features. Results show that there were significant differences between each subgroup and intact group and the density was closely correlated with pathologic changes. Therefore, the external parameter of density might offer some clues to its pathologic changes, such as edema and fibrosis.

Chelerythrine Attenuates Renal Ischemia/Reperfusion-induced Myocardial Injury by Activating CSE/H2S via PKC/NF-κB Pathway in Diabetic Rats

Background/Aims: Chelerythrine (CHE), a benzophenanthridine alkaloid, is a potent, selective, and cell-permeable protein kinase C (PKC) inhibitor. The purpose of the present study was to evaluate the effect of CHE on myocardial recovery after renal ischemia/reperfusion (I/R)-induced myocardial injury (RI/RMI) in a streptozocin (STZ)-induced diabetic rat model. Methods: Diabetes mellitus (DM) rats preconditioned with CHE and D, L-propargylglycine (PAG) were subjected to renal I/R. The extent of cardiac morphologic lesions and the biochemical markers of cardiorenal function and oxidative stress were detected utilizing hematoxylin-eosin staining, commercial kits, and enzyme-linked immunoassay, respectively. The expressions of cystathionine-γ-lyase (CSE), PKC-α, PKC-β2, and nuclear factor-kappa B (NF-κB) in the rat myocardial tissue were measured utilizing western blotting. Results: Renal I/R treatment resulted in myocardial injury. CHE-preconditioning promoted the recovery from myocardial damage by ameliorating the biochemical parameters of myocardial injury, reducing oxidative stress, increasing the H2S level, up-regulating the expression of CSE, and down-regulating the expressions of PKC-α, PKC-β2, and NF-κB. Conclusion: These findings suggest that CHE-pretreatment may exert a protective effect on the myocardium against RI/RMI by activating endogenous CSE/H2S via PKC/NF-κB pathway in STZ-induced diabetic rats. Further studies are needed defining underlying mechanisms.

The genetic polymorphisms of 9 Y-STR loci in Han population from east China

Objective To analyze sequence characteristics and genetic polymorphisms of nine new Y-chromosomal short tandem repeats (Y-STR) in Han population from Hangjiahu Plain in east China, and to evaluate their applications of forensic science. Methods Blood samples of 159 unrelated male individuals from Hangjiahu Plain Han population were genotyped by PCR. The allele and haplotype frequencies of nine Y-STR loci were calculated by the counting method. Results 9 Y-STR loci (DYS522, DYS549, DYS556, DYS565, DYS568, DYS570, DYS594, DYS593, DYS588) are single copy, and 7, 5, 5, 4, 6, 9, 4, 4, 8 alleles were detected respectively. The gene diversity (GD) value ranged from 0.1434 to 0.7994. A total of 136 haplotypes were found, and the haplotype diversity was 0.997. None of Y-STR allele mutation was observed in the 30 father/son pairs with demonstrated paternity. Conclusion The identifying power of 9 Y-STR loci was higher. Nine Y-STR loci developed by us are suitable genetic markers for forensic applications.

Effect of nitric oxide synthase inhibitors on the changes of hemodynamic parameters and aortic tension induced by septic shock in rats

In this study, we investigated the effect of three types of nitric oxide synthase inhibitors on the changes of hemodynamic parameters and thoracic aorta tension induced by septic shock in rats. We established the septic shock model rats by Cecal ligation and puncture (CLP), and nitric oxide synthase inhibitors (L-NAME, AMG and 7-NI) were injected after CLP. Mean arterial blood pressure (MABP), and ventricular dynamic parameters (heart rate, left ventricular developed pressure, maximal rise/fall velocity of ventricular pressure) were monitored. Isolated thoracic rings were mounted on an organ bath and the tension of the vessel was recorded. Results shown that after using L-NAME, AMG and 7-NI the mortality decreased to 50.0%, 37.5%, and 42.1%; MABP and ventricular dynamic parameters were decreased. The three types of nitric oxide synthase inhibitors can improve the hemodynamic parameters and vasoconstriction responsiveness of endothelium-denuded aorta of septic shock rats.

Clinical role of hWAPL in histologic diagnosis of cervical cancer and cervical intraepithelial neoplasia: Correlation with p16INK4a expression and HPV testing

In this study, we examined the expression of hWAPL in cervical intraepithelial neoplasia (CIN) I-III and cervical squamous cell carcinoma, and investigated clinical application value of hWAPL in diagnosis of cervical disease. 90 samples were collected, and two slides from each sample were immunostained using hWAPL and p16INK4a antibody. HPV-DNA testing was performed using the HC2 DNA test. The expression intensity of hWAPL in SCC group, CIN III group, CIN II group and CIN I group was obviously higher than that in normal cervix group, and the expression intensity in SCC group, CIN III group and CIN II group was higher than that in CIN I group. Based on ROC curves of hWAPL, when using ≥CIN I, ≥CIN II and ≥CIN III as cutoff for positive classification, area under the curve (AUC) was 0.815, 0.699, and 0.616, respectively. Therefore, hWAPL may be a promising candidate for diagnosis of low-grade CIN.