Xin Bai

Inonotus obliquus polysaccharide regulates gut microbiota of chronic pancreatitis in mice

Polysaccharide is efficient in attenuation of metabolic ailments and modulation of gut microbiota as prebiotics. The therapeutic effect of Inonotus obliquus polysaccharide (IOP) on chronic pancreatitis (CP) in mice has been validated in our previous study. However, it is not clear whether IOP is conducive to maintaining the homeostasis between gut microbiota and host. The aim of this study is to testify the potential effects of IOP on gut microbiota composition and diversity in mice with CP. The changes in glutathione peroxidase (GSH-PX), total antioxidant capacity (TAOC), tumor necrosis factor alpha (TNF-α), transforming growth factor beta (TGF-β), lipase and trypsin levels were measured by commercial assay kits, meanwhile the gut microbiota composition and diversity were analyzed by high throughput sequencing. The IOP treatment increased GSH-PX and TAOC levels, and decreased TNF-α, TGF-β, lipase and trypsin levels in CP mice. It was also observed that gut microbiota in IOP treated groups were less diverse than others in terms of lower Shannon diversity index and Chao 1 estimator. IOP increased the proportion of Bacteroidetes and decreased that of Firmicutes at phylum level. Bacteroidetes was found positively correlated with GSH-PX and TAOC, and Firmicutes correlated with TNF-α, TGF-β, and lipase. In conclusion, administration of IOP could regulate gut microbiota composition and diversity to a healthy profile in mice with CP, and some bacterial phylum significantly correlated with characteristic parameters.

Effect of coexisting ions on Cr(VI) adsorption onto surfactant modified Auricularia auricula spent substrate in aqueous solution

In this paper, the effect of coexisting cations and anions on Cr(VI)(in the form of Cr2O72-) adsorption onto Auricularia auricula spent substrate (AASS) modified by cetyl trimethyl ammonium bromide (CTAB) was investigated in batch adsorption experiment. The Cr(VI) adsorption capacity of 9.327 mg/g, obtained at pH 3.0, 303 K, adsorbent dosage of 2 g/L, initial Cr(VI) concentration of 20 mg/L, rotational speed of 150 r/min for 120 min, was decreased in the presence of coexisting cations and anions, among which Pb2+ and PO43- affected most by 21.79% and 12.43%, respectively. XRD, XPS and FTIR detection found that coexisting Pb2+ and PO43- would not only interfere Cr(VI) to form crystals and reduce to Cr(III), but also compete with Cr(VI) for oxygen-containing and amino groups to form Pb-O and P-NH2, respectively. In addition, the single Cr(VI) adsorption fitted with Langmuir isotherm model, while the competitive adsorption was well described by Freundlich isotherm model. Both single adsorption and competitive adsorption were in line with the pseudo-second-order kinetic model.