Xin-Ping Hui

Conjugation of substituted ferrocenyl to thiadiazine as apoptosis-inducing agents targeting the Bax/Bcl-2 pathway

Ferrocene compounds are a class of biologically active compounds that has antitumour and antifungal properties. This study investigated the induction of apoptosis in human fibrosarcoma cells (HT1080) after treatment with a series of 6-ferrocenyl-3-subsituted7H-1,2,4-triazolo[3,4-b]- 1,3,4-thiadiazine (FTFs). We found that FTFs could suppress the viability of HT1080 cells. Cell cycle analysis showed that proliferative inhibition of HT1080 cells occurred through apoptosis, as the cells were blocked in G1 phase. Moreover, mitochondrial membrane staining assay demonstrated that FTFs exposure significantly decreased mitochondrial membrane potential. Finally, under the stress of FTFs, Bax/Bcl-2 ratio in HT1080 cells was significantly increased. These results suggested that FTFs-induced apoptosis in HT1080 cells may work dependent on a Bax/Bcl-2 pathway.

A green route to the synthesis of azo compounds

Polyethylene glycol (PEG, average molecular weight 400) was nused as an absorbent for NO2, the absorptivity was found to nreach up to 97%. PEG·NO2, the resulting absorbent nproduct, proved to be a very efficient, clean and moderate oxidant, It can nconvert hydrazo derivatives 1 to the corresponding azo compounds 2 for the nfirst time. The spent PEG can be recovered and recycled after the oxidation nprocess.

Novel and Efficient Solid‐State Synthesis of 3‐Alkyl‐6‐Aryl‐s‐triazolo[3,4‐b]‐1,3,4‐thiadiazine Derivatives

Abstract A novel and efficient solid‐state synthesis of s‐triazolo[3,4‐b]‐1,3,4‐thiadiazine derivatives has been reported. Twelve 3‐alkyl‐6‐aryl‐s‐triazolo[3,4‐b]‐1,3,4‐thiadiazine derivatives have been synthesized in excellent yields with short reaction time.