Xiumei Xing

Complete mitochondrial genome sequence of northeastern sika deer (Cervus nippon hortulorum)

Abstract The complete mitochondrial genome of the northeastern sika deer, Cervus nippon hortulorum, was determined by accurate polymerase chain reaction. The entire genome is 16,434u2009bp in length and contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 control region, all of which are arranged in a typical vertebrate manner. The overall base composition of the northeastern sika deer’s mitochondrial genome is 33.3% of A, 24.5% of C, 28.7% of T and 13.5% of G. A termination associated sequence and several conserved central sequence block domains were discovered within the control region.

Genetic diversity and population genetic structure of the only population of Aoluguya Reindeer (Rangifer tarandus) in China

Abstract Aoluguya Reindeer is the only reindeer species in China and currently approximately 1000 Aoluguya Reindeer remain semi-domesticated. A relative low diversity estimate was found by investigating genetic variability and demographic history of its population. Mismatch distribution curve of its nucleotide sequences and neutral test indicate its population has not experienced expansion. Genetic diversity and population structure were also analysed by using its mtDNA and microsatellites technology. Statistical results of these analyses showed there were varying degrees of population inbreeding and suggested that gene flow existed among its populations at one time. Three mutation models were also used to detect the bottleneck effect of reindeer population. The genetic variation of eight populations is relatively small. In addition, the clustering program STRUCTURE was used to analyse Aoluguya Reindeer population structure, to determine its optimal K and first time to analyse the phylogenetic status of Aoluguya Reindeer among other reindeer subspecies. It is recommended that the government establish a natural conservation area in Aoluguya Reindeer growing geography, forbade the trade and hunting of Aoluguya Reindeer, and strengthen the protection of this endangered species.

Characterization of the complete mitochondrial genome of Rusa unicolor hainana (Artiodactyla: Cervidae)

Rusa unicolor, including seven subspecies, has been listed as a vulnerable species by the International Union for Conservation of Nature and Natural Resources. The mitochondrial genome has proven to be a highly successful resource for investigation of evolution and population genetics. In this study, the complete mitochondrial genome of Rusa unicolor hainana was sequenced and analyzed. The circle genome was found to be 16,436xa0bp in length, containing 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and one noncoding control region. Additionally, maximum likelihood and Bayesian inference trees were conducted based on the 13 PCGs of species within the Cervinae subfamily. The phylogenetic tree demonstrated that R. u. dejeani is more closely related to R. u. swinhoei than to R. u. hainana. This study could provide important molecular data for species identification and the phylogenetic relationship of R. unicolor.

Complete mitochondrial genome sequence of tarim red deer (Cervus elaphus yarkandensis)

Abstract The complete mitochondrial genome of the tarim red deer, Cervus elaphus yarkandensis, was determined by accurate polymerase chain reaction. The entire genome was 16,351u2009bp in length and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 control region, all of which were arranged in a typical vertebrate manner. The overall base composition of the northeast sika deer’s mitochondrial genome was 33.3% of A, 24.4% of C, 28.9% of T and 13.4% of G. A termination-associated sequence and several conserved central sequence block domains were discovered within the control region.

Complete mitochondrial genome sequence of northeastern red deer (Cervus elaphus xanthopygus).

Abstract The complete mitochondrial genome of the northeastern red deer, Cervus elaphus xanthopygus, was determined by accurate polymerase chain reaction. The entire genome is 16,416u2009bp in length and contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 control region, all of which are arranged in a typical vertebrate manner. The overall base composition of the northeastern red deer’s mitochondrial genome is 33.3% of A, 24.3% of C, 28.9% of T and 13.5% of G. A termination-associated sequence and several conserved central sequence block domains were discovered within the control region.

Complete mitochondrial genome sequence of Aoluguya reindeer (Rangifer tarandus)

Abstract The complete mitochondria genome of the reindeer, Rangifer tarandus, was determined by accurate polymerase chain reaction. The entire genome is 16,357u2009bp in length and contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a D-loop region, all of which are arranged in a typical vertebrate manner. The overall base composition of the reindeer’s mitochondrial genome is 33.7% of A, 23.1% of C, 30.1% of T and 13.2%of G. A termination associated sequence and several conserved central sequence block domains were discovered within the control region.