Xixiang Tang

Flavobacterium beibuense sp. nov., isolated from marine sediment

A taxonomic study was carried out on strain F44-8(T), which was isolated from a crude-oil-degrading consortium, enriched from marine sediment of the Beibu Gulf, PR China. The 16S rRNA gene sequence of strain F44-8(T) showed highest similarities to those of Flavobacterium frigoris LMG 21922(T) (93.3 %), Flavobacterium terrae R2A1-13(T) (93.3 %) and Flavobacterium gelidilacus LMG 21477(T) (93.1 %). Sequence similarities to other members of the genus Flavobacterium were <93.0 %. The dominant fatty acids of strain F44-8(T) were iso-C(15 : 0), summed feature 3 (iso-C(15 : 0) 2-OH and/or C(16 : 1)ω7c), iso-C(15 : 1) G and iso-C(17 : 0) 3-OH. The DNA G+C content of strain F44-8(T) was 38.6 mol%. These results are consistent with characteristics of members of the genus Flavobacterium. Strain F44-8(T) could, however, be readily distinguished from all known Flavobacterium species by a number of phenotypic features. Therefore, according to the phenotypic and 16S rRNA gene sequence data, strain F44-8(T) represents a novel species in the genus Flavobacterium, for which the name Flavobacterium beibuense sp. nov. is proposed (type strain F44-8(T) =CCTCC AB 209067(T) =LMG 25233(T) =MCCC 1A02877(T)).

Microbacterins A and B, New Peptaibols from the Deep Sea Actinomycete Microbacterium sediminis sp. nov. YLB-01(T)

Two new peptaibols, namely microbacterins A (1) and B (2), were isolated from the deep sea inhabited actinomycete Microbacterium sediminis spp. nov. YLB-01(T). The sequences of the amino acid residues were determined on the basis of intensive NMR and ESI-MS/MS spectroscopic analysis, in addition to the Marfeys method and CD and optical rotation data for the configurational assignment. Both 1 and 2 exhibited significant cytotoxic activities against a panel of human tumor cell lines.

Indole-based alkaloids from deep-sea bacterium Shewanella piezotolerans with antitumor activities.

Chromatographic separation of a crude extract obtained from a fermentation broth of a chemically unknown bacterium Shewanella piezotolerans WP3 collected in deep-sea yielded three new indole alkaloids namely shewanellines A (1a), B (1b) and C (2), together with 12 known indole alkaloids. The structures were unambiguously elucidated on the basis of 1D and 2D NMR (1H, 13C, COSY, HMBC, HSQC and NOESY) in association with MS and CD data. Compounds 1–4, 7, 9 and 11–14 were selected for the evaluation of their cytotoxic activities against human tumor cell lines HL-60 and BEL-7402, whereas compounds 2, 4 and 9 exhibited significant inhibition toward HL-60.

Two new compounds from marine-derived fungus Penicillium sp. F11.

Two new compounds, penicillone A (1) and penicillactam (2), were isolated together with 17 known compounds from a marine-derived fungus Penicillium sp. F11. The structures of the new compounds as well as a firstly literatural reported known compound (3) were assigned by spectroscopic methods including 1D/2D NMR and MS analysis techniques. Their cytotoxicities against HT1080, Cne2, and Bel7402 cell lines were also evaluated.

Microbacterium sediminis sp. nov., a psychrotolerant, thermotolerant, halotolerant and alkalitolerant actinomycete isolated from deep-sea sediment

An aerobic, rod-shaped, Gram-positive, oxidase- and catalase-positive bacterial isolate, strain YLB-01(T), was characterized using phenotypic and molecular taxonomic methods. 16S rRNA gene sequence analysis revealed that the isolate belonged to the genus Microbacterium and represented an evolutionary lineage that was distinct from recognized species of the genus Microbacterium. The isolate showed <97 % 16S rRNA gene sequence similarity with respect to the type strains of all of the members of the genus Microbacterium with validly published names. Cell-wall hydrolysate from the isolate contained the amino acids ornithine, alanine, glycine homoserine and glutamic acid, and the cell-wall sugars consisted of ribose, glucose and galactose. The main respiratory quinones were MK-8, 9, 10 and 11. The major cellular fatty acids were anteiso-C(15:0) (38.5 %), anteiso-C(17:0) (23.1 %) and C(16:0) (18.9 %). The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and two unidentified glycolipids. The DNA G+C content of strain YLB-01(T) was 71 mol %. On the basis of the morphological, physiological and chemotaxonomic data and the results of comparative 16S rRNA gene sequence analysis, this isolate represents a novel species of the genus Microbacterium, for which the name Microbacterium sediminis sp. nov. is proposed. The type strain is YLB-01(T) (= DSM 23767(T) = CCTCC AB2010363(T) = MCCC 1A06153(T)).

A new macrolactin antibiotic from deep sea-derived bacteria Bacillus subtilis B5

Abstract A new macrolactin derivate, 7-O-2′E-butenoyl macrolactin A (1), together with three known macrolactin compounds, macrolactin A (2), 7-O-malonyl macrolactin A (3) and 7-O-succinyl macrolactin A (4), was isolated from the bacterial strain Bacillus subtilis B5, which was isolated from the 3000 m deep sea sediment of the Southwest Pacific Ocean. The structures of the new compounds were assigned by spectroscopic methods including 1-D/2-D NMR and MS analysis techniques. Compounds 1 and 2 demonstrated antifungal activities against tea pathogenic fungi Pestalotiopsis theae and Colletotrichum gloeosporioides.

Two New Cytotoxic Indole Alkaloids from a Deep-Sea Sediment Derived Metagenomic Clone

Two new indole alkaloids, metagenetriindole A (1) and metagenebiindole A (2), were identified from deep-sea sediment metagenomic clone derived Escherichia coli fermentation broth. The structures of new compounds were elucidated by spectroscopic methods. The two new indole alkaloids demonstrated moderately cytotoxic activity against CNE2, Bel7402 and HT1080 cancer cell lines in vitro.

A novel indole alkaloid from deep-sea sediment metagenomic clone-derived Escherichia coli fermentation broth

To explore secondary metabolites in deep-sea sediment metagenomic clone-derived Escherichia coli fermentation broth, different kinds of chromatography methods were used in the isolation procedures, while the structures of the isolated compounds were assigned based on the MS analysis and their 1H and 13C NMR spectra including 2D NMR techniques such as COSY, HMQC, and HMBC experiments. As a result, a novel compound was isolated and characterized as N-{1-[4-(acetylamino)phenyl]-3-hydroxy-1-(1H-indol-3-yl)propan-2-yl}-2,2-dichloroacetamide (1). In addition, eight known compounds were also obtained. Fatty acid amide hydrolase and monoacylglycerol lipase were used to screen analgesic activity, and the new compound showed analgesic activity to some extent in pharmacological test.

New phenethylamine derivatives from Arenibacter nanhaiticus sp. nov. NH36A(T) and their antimicrobial activity.

Five new phenethylamine (PEA) derivatives (1–5) were isolated from the strain of Arenibacter nanhaiticus sp. nov. NH36AT derived from the marine sediment of the South China Sea by bioassay-guided fractionation. Their structures were elucidated by spectroscopic methods including UV, IR, HR-MS and NMR. Interestingly, compounds 1–4 existed as enantiomers, which were resolved by chiral liquid chromatography. The resolved configuration of each enantiomer was assigned by the Marfey’s method. Of these compounds, 5 showed weak antimicrobial activity against Staphylococcus aureus and Bacillus subtilis with MIC values of 0.50 and 0.25 mg ml−1, respectively.

Correction to "Microbacterins A and B, new peptaibols from the deep sea actinomycete Microbacterium sediminis sp. nov. YLB-01(T)".

T corrections have been made in the references, and the corrected citations are as follows: (1) Degenkolb, T.; Brückner, H. Chem. Biodiversity 2008, 5, 1817−1843. (2) Stoppacher, N.; Neumann, N. K. N.; Burgstaller, L.; Zeilinger, S.; Degenkolb, T.; Brückner, H.; Schuhmacher, R. Chem. Biodiversity 2013, 10, 734−743. (3) Degenkolb, T.; Kirschbaum, J.; Brückner H. Chem. Biodiversity 2007, 4, 1052−1067. (4) Mueller, P.; Rudin, D. O. Nature 1968, 217, 713−719. (5) Leitgeb, B.; Szekeres, A.; Manczinger, L.; Vaǵvölgyi, C.; Kredics, L. Chem. Biodiversity 2007, 4, 1027−1051. (7) Summers, M. Y.; Kong, F.; Feng, X.; Siegel, M. M.; Janso, J. E.; Graziani, E. I.; Carter, G. T. J. Nat. Prod. 2007, 70, 391− 396. (8) Berg, A.; Schlegel, B.; Ihn, W.; Demuth, U.; Graf̈e, U. J. Antibiot. 1999, 52, 666−669. (9) Chutrakul, C.; Alcocer, M.; Bailey, K.; Peberdy, J. F. Chem. Biodiversity 2008, 5, 1694−1706. (10) Carroux, A.; Van Bohemen, A.-I.; Roullier, C.; Robiou de Pont, T.; Vansteelandt, M.; Bondon, A.; Zalouk-Vergnoux, A.; Pouchus, Y. F.; Ruiz, N. Chem. Biodiversity 2013, 10, 772− 786. (11) Brückner, H.; Becker, D.; Gams, W.; Degenkolb, T. Chem. Biodiversity 2009, 6, 38−56. (14) Wilhelm, C.; Anke, H.; Flores, Y.; Sterner, O. J. Nat. Prod. 2004, 67, 466−468. (16) Jaworski, A.; Kirschbaum, J.; Brückner, H. J. Pept. Sci. 1999, 5, 341−351. (17) Rebuffat, S.; Goulard, C.; Hlimi, S.; Bodo, B. J. Pept. Sci. 2000, 6, 519−533. (18) Leclerc, G.; Goulard, C.; Prigent, Y.; Bodo, B.; Wrob́lewski, H.; Rebuffat, S.J. Nat. Prod. 2001, 64, 164−170. (21) Bhushan, R.; Brückner, H. Amino Acids 2004, 27, 231−247. (22) de Zotti, M.; Schievano, E.; Mammi, S.; Kaptein, B.; Broxterman, Q. B.; Singh, S. B.; Brückner, H.; Toniolo, C. Chem. Biodiversity 2010, 7, 1612−1624. (23) de Zotti, M.; Biondi, B.; Crisma, M.; Hjørringgaard, C. U.; Berg, A.; Brückner, H.; Toniolo, C. Biopolymers (Pept. Sci.) 2011, 98, 36−49.