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Dive into the research topics where Ya. A. Andreev is active.

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Featured researches published by Ya. A. Andreev.


Russian Journal of Bioorganic Chemistry | 2009

New polypeptide components from the Heteractis crispa sea anemone with analgesic activity

Sergey A. Kozlov; Ya. A. Andreev; A. N. Murashev; D. I. Skobtsov; I. A. D’yachenko; Eugene V. Grishin

Two new polypeptide components which exhibited an analgesic effect in experiments on mice were isolated from the Heteractis crispa sea tropical anemone by the combination of chromatographic methods. The APHC2 and APHC3 new polypeptides consisted of 56 amino acid residues and contained six cysteine residues. Their complete amino acid sequence was determined by the methods of Edman sequencing, mass spectrometry, and peptide mapping. An analysis of the primary structure of the new peptides allowed for their attribution to a large group of trypsin inhibitors of the Kunitz type.An interesting biological function of the new polypeptides was their analgesic effect on mammals, which is possibly realized via the modulation of the activity of the TRPV1 receptor and was not associated with the residual inhibiting activity towards trypsin and chymotrypsin. The analgesic activity of the APHC3 polypeptide was measured on the hot plate model of acute pain and was significantly higher than that of APHC2. Methods of preparation of the recombinant analogues were created for both polypeptides.


Biochemistry | 2014

Acid-Sensing Ion Channels and Their Modulators

D. I. Osmakov; Ya. A. Andreev; Sergey A. Kozlov

According to a modern look acid-sensing ion channels (ASICs) are one of the most important receptors that perceive pH change in the body. ASICs represent proton-gated Na+-selective channels, which are expressed in neurons of the central and peripheral nervous system. These channels are attracting attention of researchers around the world, as they are involved in various physiological processes in the body. Drop of pH may occur in tissues in norm (e.g. the accumulation of lactic acid, the release of protons upon ATP hydrolysis) and pathology (inflammation, ischemic stroke, tissue damage and seizure). These processes are accompanied by unpleasant pain sensations, which may be short-lived or can lead to chronic inflammatory diseases. Modulators of ASIC channels activity are potential candidates for new effective analgesic and neuroprotection drugs. This review summarizes available information about structure, function, and physiological role of ASIC channels. In addition a description of all known ligands of these channels and their practical relevance is provided.


Biochemistry | 2006

Changes in expression of genes encoding antioxidant enzymes, heme oxygenase-1, Bcl-2, and Bcl-xl and in level of reactive oxygen species in tumor cells resistant to doxorubicin.

E. V. Kalinina; N. N. Chernov; A. N. Saprin; Ya. N. Kotova; Ya. A. Andreev; V. S. Solomka; N. P. Scherbak

The relationship between expression of genes encoding key antioxidant enzymes, heme oxygenase-1, Bcl-2, and Bcl-xl and change in production of reactive oxygen species (ROS) resulting from development of resistance of cancer cells K562, MCF-7, and SKOV-3 to the prooxidant chemotherapeutic agent doxorubicin (DOX) has been studied. Significant increase in mRNA level and activity of Mn-superoxide dismutase (Mn-SOD), catalase, and selenium-dependent glutathione peroxidase-1 (GPx-1) and reduced ROS level was found in resistant K562/DOX and SKVLB cells. In contrast, no change in ROS level was observed in MCF-7/DOX cells in parallel with decrease in Mn-SOD and catalase mRNAs and corresponding activities concurrently with high increase in GPx-1 mRNA and activity. As a result of the development of resistance, a similarity was found between the change in ROS level and the change in ho-1 and bcl-2 gene expression, whereas elevation of bcl-xl gene expression was observed in all three types of resistant cells. Particular features of development of adaptive antioxidant response as well as redox-dependent change in bcl-2 gene expression under formation of DOX resistance of cancer cells of different genesis are discussed.


Russian Journal of Bioorganic Chemistry | 2012

A sea anemone polypeptide toxin inhibiting the ASIC3 acid-sensitive channel

Sergey A. Kozlov; D. I. Osmakov; Ya. A. Andreev; S. G. Koshelev; Irina Gladkikh; M. M. Monastyrnaya; E. P. Kozlovskaya; Eugene V. Grishin

A polypeptide toxin π-AnmTX Hcr 1b-1 with a molecular mass of 4537 Da was isolated from the whole extract of the sea anemone Heteractis crispa by multistage liquid chromatography. According to a homology search using the BLAST algorithm, the novel toxin was referred to the group of the known sea anemone toxins BDS and APETx with the homology of the amino acid sequence not exceeding 50%. In electrophysiological studies on the receptors expressed in Xenopus laevis oocytes the toxin inhibited the amplitude of the fast component of the integral ASIC3 current. The calculated IC50 value was 5.5 ± 1.0 μM. Among the known polypeptide blockers of ASIC3 channels the π-AnmTX Hcr 1b-1 toxin was the least potent inhibitor, which can be explained, in our opinion, by a small amount of charged amino acid residues in its structure.


Doklady Biological Sciences | 2015

Biological activity of a polypeptide modulator of TRPV1 receptor

I. A. Dyachenko; Ya. A. Andreev; Yu. A. Logashina; A. N. Murashev; Eugene V. Grishin

This paper presents data on the activity of a new APHC2 polypeptide modulator of TRPV1 receptors, which was isolated from the sea anemone Heteractis crispa. It has been shown that APHC2 has an analgesic activity, does not impair normal motor activity, and does not change body temperature of experimental animals, which has a great practical value for design of potent analgesics of a new generation. Further study of the characteristics of binding of the polypeptide to the TRPV1 receptor may show approaches to the development of other antagonists of this receptor that do not influence the body temperature.


Russian Journal of Genetics: Applied Research | 2013

Wheat antimicrobial peptides

T. I. Odintsova; T. V. Korostyleva; L. L. Utkina; Ya. A. Andreev; A. A. Slavokhotova; E. A. Istomina; V. A. Pukhal’ski; T. A. Egorov

Antimicrobial peptides (AMPs) are low-molecular-weight defense polypeptides produced in all living organisms either constitutively or upon perception of signals from pathogenic microorganisms. They are important components of the immune system in both animals and plants. AMPs differ in structure and mode of action. Most of them belong to the cysteine-rich peptides; their molecules contain even numbers of cysteine residues involved in the formation of disulfide bonds, which stabilize the peptide structure. A number of families of plant AMPs have been isolated on the basis of amino acid sequence similarity and 3D structure. Plant AMP genes can be used in engineering of disease resistance in crops and development of novel antibiotics and antimycotics. We provide a concise review of properties and gene structures of major AMP families discovered by the authors in Triticum kiharae seeds, including glycine-rich peptides, defensins, hevein-like peptides, and the so-called 4-Cys peptides.


Biochemistry | 2017

Hevein-like antimicrobial peptides of plants

A. A. Slavokhotova; A. A. Shelenkov; Ya. A. Andreev; T. I. Odintsova

Plant antimicrobial peptides represent one of the evolutionarily oldest innate immunity components providing the first line of host defense to pathogen attacks. This review is dedicated to a small, currently actively studied family of hevein-like peptides that can be found in various monocot and dicot plants. The review thoroughly describes all known pep- tides belonging to this family including data on their structures, functions, and antimicrobial activity. The main features allowing to assign these peptides to a separate family are given, and the specific characteristics of each peptide are described. Further, the mode of action for hevein-like peptides, their role in plant immune system, and the applications of these mol- ecules in biotechnology and medicine are considered.


Chemistry of Natural Compounds | 2015

Inhibitory Activity on TRP Receptors of Pentacyclic Alkaloids from the Fungus Haliclona (Gellius) sp.

E. K. Ogurtsova; Tatyana N. Makarieva; Alla G. Guzii; Pavel S. Dmitrenok; V. A. Denisenko; V. B. Krasokhin; Yu. V. Korolkova; Ya. A. Andreev; I. V. Mosharova; Eugene V. Grishin

Sponges of the genus Haliclona are sources of biologically active compounds including cyclic alkylpyridines [1], unusual sphingoid bases [2], brominated polyacetylene compounds [3], macrocyclic alkaloids [4, 5], cyclic peptides [6, 7], macrolides [8], sulfated polyhydroxysteroids [9], polycyclic amines [10], etc. [11]. We isolated the two alkaloids papuamine (1) and haliclonadiamine (2) during a study of sponges of the genus Haliclona (Chalinidae) collected during the 45th expedition of the RV Akademik Oparin in Van Phong Bay on the shores of Vietnam (12°32.8N, 109°23.6E, 20 m depth). The isolation of these compounds from sponges of the genus Haliclona collected on the shores of Indonesia [12] and New Guinea [13] was reported earlier. We first isolated 1 and 2 from a sponge of this genus in the subgenus Gellius [14].


Bioorganicheskaia khimiia | 2005

Alternative splicing of pre-mRNA encoding the Musca domestica latrophilin-like protein: Primary structures of four spliced forms of mRNA and their protein products

Ya. A. Andreev; V. N. Danilevich; Eugene V. Grishin

An internal DNA fragment (∼2000 bp) homologous to the conserved regions of genes encoding latrophilin-like proteins (LLPs) was obtained by the PCR technique using degenerate primers to these gene regions. The gene-specific primers were synthesized based on the results of sequencing of the isolated fragment, and all overlapping cDNA fragments of the llp gene encoding the Musca domestica LLP were obtained by the rapid amplification of cDNA 5′- and 3′-ends (5′- and 3′-RACE). Four alternatively spliced mRNAs were found while sequencing the obtained cDNA fragments. Two long mRNAs (∼6000 nt) differ in the structures of both the regions encoding signal peptides and 5′-terminal untranslated regions. They encode large proteins (∼1800 aa), whose domain organization is similar to that of mammalian latrophilins. Each deduced protein contains a domain with seven transmembrane strands followed by an extended cytoplasmic C-terminal domain. Two other mRNA forms are derived from these long mRNAs; they encode proteins severely truncated at their C-termini (∼900 aa). They are composed of the domain with only three transmembrane regions and a short unique cytoplasmic C-terminal domain (23 aa). The limitations and drawbacks of the existing 3′-RACE techniques found during study of the long alternatively spliced cDNAs are analyzed, and ways for overcoming these difficulties are proposed.


Pharmaceutical Chemistry Journal | 2018

Analgesic Activity of a Polypeptide Modulator of TRPV1 Receptors

I. A. D’yachenko; G. I. Belous; L. A. Skobtsova; T. Yu. Zharmukhamedova; V. A. Palikov; Yu. A. Palikova; E. V. D’yachenko; E. A. Kalabina; V. B. Rudenko; Ya. A. Andreev; Yu. A. Logashina; Sergey A. Kozlov; A. N. Yavorskii; A. N. Murashev

The activity of APHC2, a new polypeptide modulator of TRPV1 receptors that was isolated from Heteractis crispa, is studied. It was established that APHC2 possessed analgesic properties, did not disturb normal locomotor activity, and did not change body temperature and hemostasis of experimental animals. These attributes could be of great practical value for designing a new generation of efficacious analgesics. A brief increase of heart rate was observed during studies of the hemodynamic activity. Further investigation of the binding specifics of this polypeptide with TRPV1 receptors could open approaches to discovering other antagonists of these receptors.

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Eugene V. Grishin

Russian Academy of Sciences

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Sergey A. Kozlov

Russian Academy of Sciences

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A. N. Murashev

Russian Academy of Sciences

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Yu. A. Logashina

Russian Academy of Sciences

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D. I. Osmakov

Russian Academy of Sciences

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E. P. Kozlovskaya

Russian Academy of Sciences

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I. A. Dyachenko

Russian Academy of Sciences

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A. A. Slavokhotova

Russian Academy of Sciences

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Alla G. Guzii

Russian Academy of Sciences

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E. K. Ogurtsova

Russian Academy of Sciences

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