Yaligara Veeranagouda
Changwon National University
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Featured researches published by Yaligara Veeranagouda.
Fems Microbiology Letters | 2011
Yaligara Veeranagouda; Kyoung Lee; Ah Ra Cho; Kyungyun Cho; Erin M. Anderson; Joseph S. Lam
In the presence of vaporized p-cresol, Pseudomonas alkylphenolia KL28 forms specialized aerial structures (SAS). A transposon mutant of strain KL28 (C23) incapable of forming mature SAS was isolated. Genetic analysis of the C23 mutant revealed the transposon insertion in a gene (ssg) encoding a putative glycosyltransferase, which is homologous to the Pseudomonas aeruginosa PAO1 PA5001 gene. Deletion of ssg in KL28 caused the loss of lipopolysaccharide O antigen and altered the composition of the exopolysaccharide. Wild-type KL28 produced a fucose-, glucose- and mannose-rich exopolysaccharide, while the mutant exopolysaccharide completely lacked fucose and mannose, resulting in an exopolysaccharide with glucose as the major component. The mutant strain showed reduced surface spreading, pellicle and biofilm formation, probably due to the cumulative effect of lipopolysaccharide truncation and altered exopolysaccharide composition. Our results show that the ssg gene of KL28 is involved in both lipopolysaccharide and exopolysaccharide biosynthesis and thus plays an important role in cell surface properties and cell-cell interactions of P. alkylphenolia.
Microbiology | 2009
Yaligara Veeranagouda; Eun Jin Lim; Dong Wan Kim; Jin-Kyoo Kim; Kyungyun Cho; Hermann J. Heipieper; Kyoung Lee
When grown with vaporized alkylphenols such as p-cresol as the sole carbon and energy source, several isolated Rhodococcus strains formed growth structures like miniature mushrooms, termed here specialized aerial architectures (SAA), that reached sizes of up to 0.8 mm in height. Microscopic examination allowed us to view the distinct developmental stages during the formation of SAA from a selected strain, Rhodococcus sp. KL96. Initially, mounds consisting of long rod cells arose from a lawn of cells, and then highly branched structures were formed from the mounds. During the secondary stage of development, branching began after long rod cells grew outward and twisted longitudinally, serving as growth points, and the cells at the base of the mound became short rods that supported upward growth. Cells in the highly fluffy structures were eventually converted, via reductive division, into structures that resembled cocci, with a diameter of approximately 0.5 microm, that were arranged in chains. Most cells inside the SAA underwent a phase variation in order to form wrinkled colonies from cells that originally formed smooth colonies. Approximately 2 months was needed for complete development of the SAA, and viable cells were recovered from SAA that were incubated for more than a year. An extracellular polymeric matrix layer and lipid bodies appeared to play an important role in structural integrity and as a metabolic energy source, respectively. To our knowledge, similar formation of aerial structures for the purpose of substrate utilization has not been reported previously for Gram-positive bacteria.
Microbiology | 2016
Kyoung Lee; Gwang Su Ha; Yaligara Veeranagouda; Young-Su Seo; Ingyu Hwang
Pseudomonas alkylphenolica is an important strain in the biodegradation of toxic alkylphenols and mass production of bioactive polymannuronate polymers. This strain forms a diverse, 3D biofilm architecture, including mushroom-like aerial structures, circular pellicles and surface spreading, depending on culture conditions. A mutagenesis and complementation study showed that a predicted transmembrane kinase, PSAKL28_21690 (1164 aa), harbouring a periplasmic CHASE3 domain flanked by two transmembrane helices in addition to its cytoplasmic GAF, histidine kinase and three CheY-like response regulator domains, plays a positive role in the formation of the special biofilm architecture and a negative role in swimming activity. In addition, the gene, named here as bmsA, is co-transcribed with three genes encoding proteins with CheR (PSAKL28_21700) and CheB (PSAKL28_21710) domains and response regulator and histidine kinase domains (PSAKL28_21720). This gene cluster is thus named bmsABCD and is found widely distributed in pseudomonads and other bacteria. Deletion of the genes in the cluster, except forbmsA, did not result in changes in biofilm-related phenotypes. The RNA-seq analysis showed that the expression of genes coding for flagellar synthesis was increased when bmsA was mutated. In addition, the expression of rsmZ, which is one of final targets of the Gac regulon, was not significantly altered in the bmsA mutant, and overexpression of bmsA in the gacA mutant did not produce the WT phenotype. These results indicate that the sensory Bms regulon does not affect the upper cascade of the Gac signal transduction pathway for the biofilm-related phenotypes in P. alkylphenolica.
Journal of Polymer Science Part B | 2009
C. Basavaraja; Yaligara Veeranagouda; Kyoung Lee; R. Pierson; Do Sung Huh
Biodegradation | 2009
Anand S. Nayak; Yaligara Veeranagouda; Kyoung Lee; T.B. Karegoudar
Journal of Polymer Research | 2010
C. Basavaraja; Yaligara Veeranagouda; Kyoung Lee; T. K. Vishnuvardhan; R. Pierson; Do Sung Huh
Environmental Microbiology | 2009
Kyoung Lee; Yaligara Veeranagouda
International Biodeterioration & Biodegradation | 2011
M. Santoshkumar; Yaligara Veeranagouda; Kyoung Lee; T.B. Karegoudar
Process Biochemistry | 2011
Yaligara Veeranagouda; C. Basavaraja; Hyun-Sook Bae; Kwang-Hyeon Liu; Kyoung Lee
Bulletin of The Korean Chemical Society | 2008
C. Basavaraja; Yaligara Veeranagouda; Kyoung Lee; R. Pierson; M. Revanasiddappa; Do Sung Huh