Yang Chunzheng

Detection and analysis of multidrug resistance in 100 cases of acute leukemia

Bone marrow specimens from 100 cases of acute leukemia (AL) diagnosed by MIC were detected with fluorescence microscopy for their mdr-1 expression using monoclonal antibody JSB-1 against P-glycoprotein (Pgp). The results showed that almost all subtypes of AL had Pgp expression and M5 of ANLL had a significantly higher expression rate in the newly diagnosed group. The MDR expression highly correlated with the clinical drug resistance and prognosis. The positive rate of Pgp (20.8%±14.9%) and MDR expression (78.9%) in refractory group were significantly higher than those in the newly diagnosed group (7.5%±9.8% and 18.2%), respectively. Cases with MDR+ expression had poor response to chemotherapy and bad prognosis.