Yang-Kwang Fan

Effect of Monochromic Light-emitting Diode Light with Different Color on the Growth and Reproductive Performances of Breeder Geese

The purpose of this study was to investigate the effect of monochromic light-emitting diode (LED) light with different color on the growth and reproductive performances of white Roman breeder geese. A randomized complete batch design was utilized for the trial, and the replicate was regarded as one batch. Twenty ganders and fifty-five dames were used in batch 1 (started on 2011/6/17 and ended on 2012/1/31), thirty ganders and eighty-four dames were used in batch 2 (started on 2012/3/23 and ended on 2012/10/26), and thirty ganders and seventy-two dames were used in batch 3 (started on 2013/3/12 and ended on 2013/12/20). Two hundred and ninety-one geese were randomly assigned to 6 rooms in an environmentally controlled house. They were randomly allotted into one of three monochromatic light treatments: Blue, red, or white. The results showed that there was no significant difference in body weight among the three lighting groups at any point throughout the experimental period. However, compared to the blue light group, significantly more eggs were produced by the red and white light groups (p<0.05). Furthermore, the laying period of the red light group was significantly longer than that of other two groups (p<0.05). In conclusion, our results suggested that red LED-light has the best effect on reproductive performance (i.e. longer laying period and higher total eggs number) at 30 lux light intensity, and is therefore a better choice for the management of breeding geese than blue or white LED-light.

In vitro antioxidant capability and performance assessment of White Roman goose supplemented with dried Toona sinensis

ABSTRACT Toona sinensis (TS) leaf is a well-known traditional oriental medicine herb. The purpose of this study was to evaluate in vitro antioxidant capability and performance assessment of White Roman goose supplemented with dried TS leaves (TSL). The TSL extracts contained 22.23 ± 1.13 mg gallic acid equivalent/g dry weight (DW) and 1.38 ± 0.06 mg quercetin equivalent/g DW of total phenolics and flavonoid contents. The scavenging action of 1, 1-diphenyl-2-picrylhydrazyl free radical and superoxide anion was as good as that of the butylated hydroxytoluene (BHT) and ascorbic acid, respectively. Inhibition of lipid peroxidation of TSL extracts at 2.5 mg/mL reached nearly 40% compared with the BHT. One hundred and eight White Roman geese aged 6 weeks were distributed to six pens randomly, and fed a grower diet ad libitum during the growing period, with each pen containing nine males and nine females following completely randomized design. Diets were supplemented with the following levels of dried TSL: 0% (control), 0.1% or 0.2% groups for 6 weeks, respectively. The results revealed no significant effects among treatments in the growth performance, blood biochemical parameters and muscle fibre of the grower geese. Superoxide dismutase (SOD) content of the serum in the 0.2% TSL group was significantly higher than that of the control group. In conclusion, dried TSL powder has antioxidative effects in vitro and could serve as a promising natural feed additive to improve the serum SOD content of grower geese.

Factors Affecting the Incidence of Angel Wing in White Roman Geese: Stocking Density and Genetic Selection

The present study investigated stocking density and genetic lines, factors that may alter the severity and incidence of angel wing (AW), in White Roman geese. Geese (n = 384) from two genetically selected lines (normal- winged line, NL, and angel-winged line, AL, respectively) and one commercial line (CL) were raised in four pens. Following common commercial practice, low-stocking-density (LD), medium-stocking-density, and high-stocking-density treatments were respectively administered to 24, 32, and 40 geese per pen at 0 to 3 weeks (1.92 m2/pen) and 4 to 6 weeks (13.2 m2/pen) of age and to 24, 30, and 36 geese at 7 to 14 weeks (20.0 m2/pen) of age. The results revealed that stocking density mainly affected body weight gain in geese younger than 4 weeks, and that geese subjected to LD had a high body weight at 2 weeks of age. However, the effect of stocking density on the severity score of AW (SSAW) and incidence of AW (IAW) did not differ significantly among the treatments. Differences were observed among the genetic stocks; that is, SSAW and IAW were significantly higher in AL than in NL and CL. Genetic selection generally aggravates AW, complicating its elimination. To effectively reduce IAW, stocking density, a suspected causal factor, should be lower than that presently applied commercially.

Effects of short light regimes and lower dietary protein content on the reproductive performance of White Roman geese in an environment-controlled house

The objective of this study is to investigate the effects of short light regimes and lower dietary protein content on the reproductive performance of White Roman geese in an environment- controlled house. Thirty-two ganders and 80 geese during the third laying period were allotted into 16 pens, randomly assigned into a split-plot design with two different lighting regimes: (1) short light regimes (SL) with 6.5h of light and 17.5h of dark (6.5L:17.5D), and (2) long light regimes (LL) with 19L:5D during the 6-wk prelaying period, followed by two different levels of protein diets (Low CP: 15% vs. High CP: 18%) for the laying period. The results showed that birds treated with the SL light regime had a heavier body weight compared to those treated with LL at the arrival of the peak period of egg production (6.19 vs. 5.87kg, P<0.05). Geese under LL had a longer laying period than those under SL treatment (277 vs. 175day, P<0.05), while the geese under SL treatment had a higher laying intensity (15.4% vs. 12.6%, P<0.05), fertility and hatchability than those under LL treatment. Our results suggest that the White Roman geese treated with 6-wk short light regime during the prelaying period and on the low CP diet during the laying period found conditions sufficient to sustain their regular reproduction performance, which would benefit geese farmers in the perspectives of energy saving and prolonged laying period.

Nonylphenol reduces sperm viability and fertility of mature male breeders in Brown Tsaiya ducks (Anas platyrhynchos)

The aim of this study was to investigate the effect of nonylphenol (NP), a widely used surfactant, on the reproductive performance of male Brown Tsaiya ducks (Anas platyrhynchos) (MBBTDs). Mature MBBTDs (n=100) were treated with NP by daily gavaging of 0, 1 (NP1), 10 (NP10) and 250 (NP250) mg/kg-BW/d for 14 wk. Semen quality, fertilization rate and specific factors in blood plasma were measured. Weights of organs were also measured at 14 wk after NP administration. Ducks from each treatment (n=4) were continually treated with NP thereafter for 12 mo to observe changes of tissue ultrastructure by microscopic examination. The results showed that ducks treated with amounts of NP of greater than 1mg NP/kg BW/d (NP1) for 14 wk had decreased sperm viability (32.3%) compared to those in the control group (74.1%, P<0.05). The fertilization rate of ducks treated with 250mg NP/kg-BW/d (NP250) for 14 wk was reduced (21.0%) compared to the control group (74.5%, P<0.05). Plasma aminotransferase (AST) and alanine aminotransferase (ALT) activities were also greater in NP250 group at the 14th wk post-treatment. Plasma testosterone concentrations were increased by NP1 treatment at the 14th wk post-treatment. Administration at dosage 250mg NP/kg-BW/d for 12 mo resulted in reduced sperm counts (P<0.05) and histopathological changes, such as dilated seminiferous tubules (P<0.05) and degenerated spermatocytes (P<0.05). These findings strongly suggest that NP adversely affects the reproductive performance of MBBTDs.

Physiologic Responses of Mammalian Oocytes and Embryos under Thermal Stress: An Overview

Animals respond to environmental stresses by a global adaptation and adjustment to their physiologic homeostasis in order to eliminate most harmful changes and survive from the insults. This adaptation is the summation of all responses from their building blocks, cells with their associated cellular machineries in response to the undesired environmental clues. It has been known that heat shock proteins (Hsps), some of which are molecular chaperones present in all organisms, are a group of universal cellular proteins to oppose environmentally induced denaturation of many other proteins. These proteins function to assist in folding of newly synthesized proteins and maintain preexisting proteins in a stable conformation without aggregation under stress conditions, which are essential for thermal adaptation in prokaryotes and eukaryotes, including mammalian cells. Although thermobiology has been one of the active fields of study in cell biology or cancer therapy, there is less information available for the adaptation or responses to thermal stresses in embryonic cells such as oocytes and embryos. Previous studies have shown that a short-term heat shock (HS) impacts the developmental competence of embryos during the early phase of apoptosis and the alteration of intracellular calcium concentrations of matured porcine oocytes. Cleavage and blastocyst rates declined while the Ca^(2+)-releasing ability of matured oocytes was enhanced by a short duration (2 h) of HS, but declined after prolonged heat exposure. Taken together, the mechanisms of physiologic adaptation in response to thermal stress in oocytes and embryos are complex processes. HS can cause multiple changes to the oocyte and developing embryos such as enzymatic reactions, ionic influxes, DNA structure and cytoskeleton reorganization, as well as changes in the ooplasmic [Ca^(2+)]i after various intensities of HS. These phenomena may be critical parameters to evaluate their developmental competence. Delicate equilibrium between the deleterious effects and thermotolerance thermotolerance of oocytes, embryos and even the whole animal adapting to HS is one of the decisive factors in determining their fate during the course of development.

Characterizing early embryonic development of Brown Tsaiya Ducks (Anas platyrhynchos) in comparison with Taiwan Country Chicken (Gallus gallus domestics)

Avian embryos are among the most convenient and the primary representatives for the study of classical embryology. It is well-known that the hatching time of duck embryos is approximately one week longer than that of chicken embryos. However, the key features associated with the slower embryonic development in ducks have not been adequately described. This study aimed to characterize the pattern and the speed of early embryogenesis in Brown Tsaiya Ducks (BTD) compared with those in Taiwan Country Chicken (TCC) by using growth parameters including embryonic crown-tail length (ECTL), primitive streak formation, somitogenesis, and other development-related parameters, during the first 72 h of incubation. Three hundred and sixty eggs from BTD and TCC, respectively, were incubated at 37.2°C, and were then dissected hourly to evaluate their developmental stages. We found that morphological changes of TCC embryos shared a major similarity with that of the Hamburger and Hamilton staging system during early chick embryogenesis. The initial primitive streak in TCC emerged between 6 and 7 h post-incubation, but its emergence was delayed until 10 to 13 h post-incubation in BTD. Similarly, the limb primordia (wing and limb buds) were observed at 51 h post-incubation in TCC embryos compared to 64 h post-incubation in BTD embryos. The allantois first appeared around 65 to 68 h in TCC embryos, but it was not observed in BTD embryos. At the 72 h post-incubation, 40 somites were clearly formed in TCC embryos while only 32 somites in BTD embryos. Overall, the BTD embryos developed approximately 16 h slower than the chicken embryo during the first 72 h of development. To our best knowledge, this is the first study to describe two distinct developmental time courses between TCC and BTD, which would facilitate future embryogenesis-related studies of the two important avian species in Taiwan.