Yang Mengsu

Effects of lanthanum and gadolinium on proliferation and differentiation of primary osteoblasts

The effects of La3+ and Gd3+ on the proliferation, differentiation and adipogenic transdifferentiation of rat calvarial os-teoblast-like cells (ROB cells) were evaluated by MTT method, measuring the activity of alkaline phosphatase (ALP) and Oil red O measurement. Both of La3+ and Gd3+ inhibited the proliferation of ROB cells at all concentrations (1×10−5, 1×10−6 , 1×10−7, 1×10−8, 1×10−9 mol L−1,. La 3+ at concentration of 1×10−5 mol, L−1 significantly increased the alkaline phosphatase activity of ROB cells up to 3 folds, (P<0.01). However, the effects reversed to inhibit at other concentrations. Gd3+ played a negative role in the alkaline phosphatase activity. La 3+ inhibited the adipogenic transdifferentiation of ROB cells at all concentrations in a dose-dependent way. However, Gd3+ promoted the adipogenic transdifferentiation of ROB cells at 1×10−8 and 1×10−9 mol L−1. These findings suggested that the effects of rare earth elements on the proliferation, differentiation and adipogenic transdifferentiat...

GENE EXPRESSION PROFILING IN MULTIDRUG RESISTANT KB CELLS USING cDNA MICROARRAYS

Objective: A single mechanistic pathway cannot explain the genesis of drug resistance in cancer. Drug resistance in cancer is a major obstacle to successful chemotherapy. KB cells provide a useful starting point for selection of the multidrug resistant (MDR) cell lines. Methods: We used cDNA microarrays containing 12,720 sequences of known genes, expressed sequence tags and unknown clones to monitor gene expression profiles in MDR KB cells. Results: Preliminary data analysis showed that 18 genes were up-regulated and 18 genes were down-regulated by comparison of expression patterns between KB 3-1 and MDR KB-V1 cells. Furthermore, the highly over-expressed CGA, CLU genes in MDR KB-V1 cell were verified with conventional Northern blot analysis. These genes contain information predictive of drug resistance of cancer cells. Conclusion: Our study demonstrates that genome-wide gene expression profiling by using cDNA microarray technique is a valuable approach in obtaining molecular mechanism of drug resistance in cancer cells.

Effects of water extract of Cajanus cajan leaves on differentiation and function of primary osteoblasts and osteoclasts in vitro

The effects of water extract of Cajanus cajan (L.) Millsp. leaves (WECML) on differentiation and function of primary osteoblasts and osteoclasts were studied. The results indicated that WECML inhibited the proliferation of osteoblasts at higher concentration of 100 μg/mL and the inhibition rate was 12.12%. When its concentration descended to 0.01~10 μg/mL, it turned to stimulate the proliferation of the osteoblasts. The effects of WECML on differentiation of osteoblasts depended on concentrations and time. It promoted the mineralization function of osteoblasts at concentrations of 0.01, 0.1, and 1 μg/mL, but inhibited the mineralization function at concentrations of 10 and 100 μg/mL. It inhibited bone resorption activity as indicated by the dose-dependent reduction in the pit numbers and areas (P < 0.05). This indicates that the WECML may be a potential source of natural anti-osteoporosis products.

Effects of genistein, daidzein and glycitein on the osteogenic and adipogenic differentiation of bone marrow stromal cells and on the adipogenic trans-differentiation of osteoblasts*

Abstract The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT), alkaline phosphatase (ALP) activity and oil red O assays were used to examine the effects of genistein, diadzein and glycitein on the osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells (MSCs) and the adipogenic trans-differentiation of primary mouse osteoblasts. The results indicated that daidzein, genistein and glycitein at concentrations from 1 × 10−8mol/L to 1×10−5 mol/L promoted the proliferation of MSCs and osteoblasts; genistein, daidzein and glycitein promoted osteogenic differentiation and inhibited adipogenic differentiation of MSCs, and inhibited adipocytic transdifferentiation of osteoblasts at appropriate concentrations as 17β-estradiol. It suggests that genistein, daidzein and glycitein regulate a dual differentiational process of MSCs into the osteogenic and adipogenic lineages, and trans-differentiational process of primary osteoblasts into the adipocyte lineages, causing a ...