Yang Shulin

Effect of knockdown TAGLN on the migration capacity of Wuzhishan pig's bone marrow mesenchymal stem cells.

Mesenchymal stem cells (MSCs) are multipotent adult stem cells, can easily be isolated from the bone marrow and subsequently expand in vitro. Bone marrow MSCs (BMMSCs) appear to be poorly immunogenic, and are the most widely used MSCs in tissue regenerative medicine, and the migration capacity of BMMSCs is the key determinant for the efficiency of their regenerative therapy. The Wuzhishan pig (WZSP) is characterized by its physiological and general biochemical indices very similar to humans, and may be potential organ donors for human being owing to its size. In this study, the BMMSCs from WZSP were used to study their migration capacity which regulated by TAGLN in vitro. The specific short hairpin RNA (shRNA) for TAGLN was designed to knock down TAGLN gene, and the scratch assay and transwell migration assay was employed to estimate effect of knockdown TAGLN on the migration capacity of BMMSCs from WZSP. The results showed that the specific shRNA for knocking down TAGLN efficiently was found for the BMMSCs, and there was a significant effect on the migration capacity of the BMMSCs from WZSP with knockdown TAGLN in vitro. In conclusion, TAGLN was an important factor in maintaining the migration capacity of the BMMSCs, which may be benefit for the BMMSCs from WZSP to be used in regenerative therapy for human being.