Yaohui Hu

Methyl jasmonate- and salicylic acid-induced d-chiro-inositol production in suspension cultures of buckwheat (Fagopyrum esculentum)

The aim of this work is to investigate the effects of methyl jasmonate (MeJ) and salicylic acid (SA) on d-chiro-inositol (DCI) production in buckwheat (Fagopyrum esculentum) suspension cultures. In this study, adding optimal concentrations of MeJ and SA at an appropriate time markedly increased DCI production (yield 6.141 and 5.521xa0mg/gxa0DW, respectively). In addition, treatment of buckwheat cultures with a combination of 0.2xa0mM MeJ and 0.6xa0mM SA on daysxa00 and 9 increased the DCI yield to 7.579xa0mg/gxa0DW, which was 3.726 times higher than that in the control; furthermore, the former yield was higher than that achieved by the addition of either elicitor alone. Moreover, unlike MeJ, SA did not exert a negative effect on cell growth.