Yashbir Singh Shivay

Cyanobacteria mediated plant growth promotion and bioprotection against Fusarium wilt in tomato

Cyanobacteria - phytopathogenic fungi - tomato plant interactions were evaluated for developing suitable biological options for combating biotic stress (Fusarium wilt) and enhancing plant vigour. Preliminary evaluation was undertaken on the fungicidal and hydrolytic enzyme activity of the cyanobacterial strains (Anabaena variabilis RPAN59, A. laxa RPAN8) under optimized environmental/nutritional conditions, followed by amendment in compost-vermiculite. Such formulations were tested against Fusarium wilt challenged tomato plants, and the Anabaena spp. (RPAN59/8) amended composts significantly reduced mortality in fungi challenged treatments, besides fungal load in soil. Cyanobacteria amended composts also led to an enhancement in soil organic C, nitrogen fixation, besides significant improvement in growth, yield, fruit quality parameters, N, P and Zn content. The tripartite interactions also enhanced the activity of defence and pathogenesis related enzymes in tomato plants. A positive correlation (r = 0.729 to 0.828) between P content and pathogenesis/defense enzyme activity revealed their role in enhancing the resistance of the plant through improved nutrient uptake. Light and scanning electron microscopy (SEM) revealed cyanobacterial colonization, which positively correlated with reduced fungal populations. The reduced disease severity coupled with improved plant growth/ yields, elicited by cyanobacterial treatments, illustrated the utility of such novel formulations in integrated pest and nutrient management strategies for Fusarium wilt challenged tomato crop.

Evaluating the efficacy of cyanobacterial formulations and biofilmed inoculants for leguminous crops

Our investigation was aimed towards evaluating the agronomic potential of biofilmed preparations (developed using Anabaena/Trichoderma as matrices with different agriculturally useful bacteria/fungi as partners) and selected cyanobacterial strains (Anabaena laxa (T7) RP8/Calothrix sp.). The formulations were prepared using paddy straw compost:vermiculite (1:1) as carrier and tested as inoculants in mungbean and soybean. The effects of the formulations were evaluated in terms of microbiological, nutrient availability, and plant biometric parameters. The Trichoderma viride–Bradyrhizobium biofilm exhibited 20–45% enhancement in fresh/dry weight of plants over other microbial treatments, while the T. viride–Azotobacter biofilm exhibited highest dehydrogenase activity in the soil and nitrogen fixation. T7 RP8 recorded statistically at par yield values with the T. viride–Bradyrhizobium (T5) biofilm treatment in mungbean. In soybean, among all the treatments, the T5 biofilm recorded the highest fresh weight of plants and available N in soil at harvest. The Anabaena–T. viride biofilmed formulations proved to be the most promising for soybean, recording 12–25% enhanced yield and microbial activity (measured as dehydrogenase activity). This study highlights the promise of cyanobacterial inoculants and biofilmed biofertilizers as promising inputs for integrated nutrient management strategies in agriculture.

Enhancing micronutrient uptake and yield of wheat through bacterial PGPR consortia

A pot experiment was undertaken under net house conditions, with three rhizobacterial strains AW1 (Bacillus sp.), AW5 (Providencia sp.) and AW7 (Brevundimonas sp.), applied along with 2/3 recommended dose of nitrogen (N) and full dose of phosphorus (P) and potassium (K) fertilizers (N90P60K60). An enhancement of 14–34% in plant biometric parameters and 28–60% in micronutrient content was recorded in treatments receiving the combination of AW1 + AW5 strains, as compared to full dose of fertilizer application. The treatment involving inoculation with AW5 + AW7 recorded highest values of % P and N, with a two-fold enhancement in phosphorus and 66.7% increase in N content, over full dose application of P and K fertilizers. A significant correlation was recorded between plant biomass, panicle weight, grain weight, N, P and iron (Fe) with acetylene reduction activity, indicating the significance of N fixation in overall crop productivity. Our study illustrates the multiple benefits of plant growth promoting rhizobacteria (PGPR) inoculation in integrated nutrient management and biofortification strategies for wheat crop.

EVALUATING THE ESTABLISHMENT AND AGRONOMIC PROFICIENCY OF CYANOBACTERIAL CONSORTIA AS ORGANIC OPTIONS IN WHEAT–RICE CROPPING SEQUENCE

Cyanobacteria represent promising organic inputs in rice–wheat cropping system, as they contribute towards accretion of N and C, besides secreting growth-promoting substances which influence plant productivity and soil fertility. The present study focused towards using a combinatorial approach for evaluating field-level colonization of cyanobacteria in soil and their effect on soil microbiological and plant parameters, employing agronomic and molecular tools. A consortium of cyanobacterial strains (BF1, Anabaena sp., BF2, Nostoc sp., BF3, Nostoc sp. and BF4, Anabaena sp.) was employed in different three-and four-member combinations along with 75% N + Full dose of P and K fertilizers. A significant enhancement in microbial activity and plant growth/yields and savings of 25% N in the wheat–rice cropping sequence were recorded, especially in treatments involving 75% N + Full dose of PK+BF1+BF2+BF4 and T5, i.e. 75% N + Full dose of PK+BF1+BF2+BF3. Such treatments were significantly higher or statistically at par with fertilizer controls – 75% N + Full dose of PK fertilizers. The use of DNA-based markers further helped to establish the colonization of the inoculated cyanobacteria, especially BF2 and BF3 strains. Our study clearly illustrated the establishment of inoculated cyanobacterial strains and their role in enhancing the crop productivity and soil health of the rice–wheat cropping system.

Chapter Two - Agronomic Biofortification of Cereal Grains with Iron and Zinc

Abstract Iron and zinc deficiencies in human nutrition are widespread in developing Asian and African countries where cereal grains are the staple food. Effects are therefore underway to develop cereal genotypes with grains denser in Fe and Zn by traditional plant breeding or using genetic engineering techniques. This approach requires a long period and adequate funds. However, the products of genetic engineering are not well accepted in many countries. Also, there is a trade-off between yield and grain biofortification. Agronomic biofortification offers to achieve this without sacrificing on yield and with no problem of product acceptance. From the viewpoint of biofortification, foliar application has been reported to be better than the soil application of Fe and Zn, and for this purpose, chelated Fe and Zn fertilizers are better. When soil applied, water soluble sources of Zn are better. Soil application of Fe is not recommended. Agronomic biofortification depends upon management practices (tillage, water management, nutrient interactions), soil factors (amounts present, pH, mechanisms of Zn fixation other than pH), and plant factors (root characteristics, excretion of phytosiderophores and organic acids by roots, Zn utilization at the cellular level, translocation within plant and mechanisms of Zn accumulation in grain). Genetic and agronomic biofortification are complementary to each other. Once the genotypes having denser grains are developed, they will have to be adequately fertilized with Fe and Zn. However, much more research in agronomy, soil science, and plant physiology is needed to understand the complex soil–plant–management interaction under different agroecological conditions under which cereals are grown. The situation is more complex for rice, which is grown under flooded, upland, and intermediate water conditions.

Micronutrient enrichment mediated by plant-microbe interactions and rice cultivation practices

ABSTRACT A field experiment was conducted to evaluate the effect of different plant growth promoting microorganisms (PGPM) on micronutrient enrichment of rice crops grown under conventional (flooded) and SRI (System of Rice Intensification) practices. Significant differences were recorded among treatments and cultivation practices in terms of soil microbial activity reflected in enhanced nutrient uptake, enzyme activity, and yield. The Anabaena-based biofilm inoculants were particularly superior under both methods of cultivation, leading to 13–46% enhancement of iron and 15-41% enhancement of zinc in rice grains over uninoculated controls. SRI was found to be superior in terms of enhancing the concentration of zinc, copper, iron, and manganese (Zn, Cu, Fe and Mn), particularly in grains, and significant in increasing the activity of defense- and pathogenesis- related enzymes and yield parameters. This study illustrates the utility of cyanobacteria-based inoculants for both methods of rice cultivation and their significant interactions with the plant, leading to micronutrient enrichment of rice grains. Such formulations can complement the current biofortification strategies and help in combating the problems of malnutrition globally.

Rhizospheric Flora and the Influence of Agronomic Practices on Them: A Review

Plants and most of the microorganisms in the rhizosphere have symbiotic relationships. While rhizodeposits (root exudates having lysates, mucilages) provide the food and influence the structure and number of microorganisms in the rhizosphere, the latter benefit the plants through secretion of a number of growth promoting hormones, organic acids and siderophores that help in increased availability and uptake of nutrients by plants. The interactions of roots and microflora may influence the plant growth positively through a variety of mechanisms, including fixation of atmospheric nitrogen by different classes of proteobacteria, increased biotic and abiotic stress tolerance imparted by the presence of endophytic microbes, and direct and indirect advantages imparted by plant growth-promoting rhizobacteria. The soil microorganisms affect plant growth, and are affected by plant growth, but there is incomplete understanding of their cumulative and interactive effects on plant performance, especially under varied crop production regimes. The diversity of cropping systems in both time and space (by rotations, intercropping, and so on) creates a mosaic of soil resources and niches, which in turn, enhances belowground biodiversity and improves the resilience of the system as a whole. Therefore, agronomic practices such as crop rotation, tillage, addition of organic manures, chemical fertilizers and mulches influence the structure and number of microorganisms in the rhizosphere. However, very little data are available on this subject. There is a need to generate such data to develop a strategy for sustainable agriculture.

Cyanobacterial inoculation elicits plant defense response and enhanced Zn mobilization in maize hybrids

Abstract The present investigation evaluated the effect of inoculating different cyanobacterial formulations on a set of hybrids of maize, in terms of plant defense enzyme activity, soil health parameters, Zn concentration, and yields. Microbial inoculation showed significant effects on accumulation of Zn in flag leaf, with A4 (Anabaena–Azotobacter biofilm) recording the highest values. Analysis of variance (ANOVA) indicated that both the hybrids and cyanobacterial treatments brought about significant variation in terms of glomalin-related soil proteins and polysaccharides in soil and the activity of defense enzymes in roots and shoots of the plants. Cyanobacterial inoculants—A4 (Anabaena–Azotobacter biofilm) and A1 (Anabaena sp.–Providencia sp., CW1 + PW5) enhanced the activity of peroxidase, PAL and PPO in roots, which also showed a positive correlation with Zn concentration in the flag leaf. Grain yield ranged from 7.0 to 7.29 t/ha among the different inoculants. Comparative analyses of treatments showed that A3 (Anabaena–Trichoderma-biofilmed formulation) and hybrid B8 (Bio-9681) were superior in terms of parameters investigated. This represents the first report on the genotypic responses of maize hybrids to cyanobacteria-based inoculants. Future research should focus on dissecting the role of root exudates and cyanobacteria-mediated Zn mobilization pathway in maize.

ZINC-COATED UREA IMPROVES PRODUCTIVITY AND QUALITY OF BASMATI RICE (ORYZA SATIVA L.) UNDER ZINC STRESS CONDITION

A field study conducted for two years (2006 and 2007) at the Research Farm of the Indian Agricultural Research Institute, New Delhi, India showed that zinc (Zn) fertilization increased yield attributes, grain and straw yield, enhanced Zn concentrations and its uptake and improved kernel quality before and after cooking in basmati rice ‘Pusa Sugandh 5’. A 2% Zn-coating with zinc sulfate (ZnSO4·7H2O) was found to be the best but a 2% Zn-coating with zinc oxide (ZnO) was very close to it in terms of grain and straw yield and Zn concentrations in basmati rice grain and straw under Zn stress conditions. Partial factor productivity (PFP) of applied Zn varied from 984–3,387 kg grain kg Zn−1, agronomic efficiency (AE) varied from 212–311 kg grain kg−1 Zn (applied) and physiological efficiency (PE) of Zn varied from 6,384–17,077 kg grain kg−1 Zn (absorbed). Thus, adequate Zn fertilization of basmati rice can lead to higher grain yield and Zn-denser grains with improved cooking quality in basmati rices under Zn stress soil conditions.

Relative efficiency of zinc oxide and zinc sulphate-enriched urea for spring wheat

Field experiments conducted at the Indian Agricultural Research Institute, New Delhi during 2005–2007 showed that the application of small amounts of zinc (Zn) in the form 0.5–2.0% of Zn-enriched urea significantly increased yield attributes, grain and straw yield, Zn concentrations in the grain and straw and Zn uptake by spring wheat. The agronomic and crop recovery efficiency of applied Zn decreased as the level of Zn-enrichment was increased from 0.5 to 2%. Zinc sulphate (ZnSO4) and zinc oxide (ZnO) were equally effective in increasing the grain yield of wheat. Based on these results, we recommend the application of a 0.5–1.0% Zn enrichment of urea with ZnSO4 or a 1.0% Zn-enrichment with ZnO.