Yasuhiko Sugii

Three Dimensional Shape Measurement of Micro Droplet Utilizing Micro LIF Technique

This paper describes a novel measurement technique for three dimensional shape of micro droplet utilizing micro LIF (Laser Induced Fluorescence) technique. A measurement system consisted of microscope equipped with 10x lens, high sensitive CCD camera, CW Nd:YAG laser, optical filters and so on. Calibration curve between thickness of micro droplet and emission of fluorescence dye solution was obtained by the use of the PDMS (polydimethylsiloxane) microchip, whose depth varied from 8 to 120 μm fabricated using photo lithography technique. The microchip based calibration method provides high measurement accuracy and eliminate photobreaching effect. Measurement accuracy of the present method in depth direction was about 2 μm assessed using laser displace meter and the spatial resolution in the x-y plane became 6.7 μm. The technique is useful to investigate a two phase flow in micro scale.Copyright

Velocity Measurement of In Vitro Blood Flow in Microchip Cultured Endothelial Cells

In order to investigate vascular diseases such as cause of atherosclerosis and myocardial infarction, relationships of endothelial cells (ECs) covered with surface blood vessels and blood flow stimulation have been experimentally studied. In the study, a blood vessel model for in vitro experiment made from polydimethylsiloxane (PDMS) microchip with a straight microchannel with 400 μm width and 100 μm depth was developed. By optimizing cells cultured condition such as the liquid introduction method and the surface coating for enhancement of cell attachment on the microchannel wall, cell culture method in the microchip were developed. Velocity distributions on the ECs surface in the blood vessel model were measured using micro PIV technique. Measured velocity vectors on the ECs surface were fluctuated caused by the three dimensional effect of the cell shape.Copyright

Flow Visualization of Liquid/Liquid Optical Waveguide Microfluidic Device

A liquid/liquid optical waveguide microfluidic device made of PDMS (polydimethylsiloxane) was developed in order to investigate chemical reaction and mass-transfer such as extraction, adsorption, mixing and diffusion at the liquid/liquid interface. In the micro scale, since the scale effect that the gravity force can be negligible and flow become laminar was appeared, a stable liquid / liquid sheath flow can be generated. In order to investigate the flow field concentration distributions of molecule in the present microfluidic device were measured using micro LIF (laser induced fluorescence) technique. The measurement results show the stable and long interface of miscible liquid flow.Copyright