Yasuhiro Hayashi

Intramolecular Radical Cyclization of 2-Haloethanal Allyl Acetal and Allyl 2-Halophenyl Ether with a Grignard Reagent in the Presence of Iron(II) Chloride

Treatment of 2-iodoethanal alkenyl acetals, generated by iodoetherization from butyl vinyl ether and allylic alcohols, with phenylmagnesium bromide in the presence of a catalytic amount of FeCl2 provided tetrahydrofuran derivatives in good yields. Allyl 2-halophenyl ethers also afforded dihydrobenzofuran derivatives upon treatment with phenylmagnesium bromide under FeCl2 catalysis.

Fibroblast growth factor-stimulated growth of porcine aortic endothelial cells depends on hypoxanthine in fetal bovine serum in culture media

The rate of proliferation of porcine aortic endothelial cells (PAEC) in response to stimulation of fibroblast growth factors (FGFs) was largely retarded in media supplemented with 10% dialyzed fetal bovine serum (FBS) in place of nondialyzed FBS. This inhibition was overcome by supplement of dialyzable fraction, and hypoxanthine was purified from the dialyzable fraction as the active compound which stimulated the basal and FGF-dependent growth rates of dialyzed FBS-treated PAEC. Addition of hypoxanthine (5 microM) to media with 10% dialyzed FBS containing FGFs (10 ng/ml) markedly increased the rate of both cell proliferation and DNA synthesis of PAEC, and their maximal levels were comparable to those attained by cells in media with 10% nondialyzed FBS. Hypoxanthine changed the spindle-like morphology of dialyzed FBS-treated PAEC even in the presence of FGFs into the cobblestone-like morphology of regular PAEC in media with 10% FBS.

Consecutive vibration characteristics monitoring of high-rise steel building

This paper reported the continuous seismic observation for a super-high-rise steel building in Osaka basin, Japan. We observed 16 earthquake records including the Tohoku earthquake on March 11, 2011. The signal to noise ratio is sufficiently good for all records. We estimated natural period and impulse response from those seismograms, and showed slight frequency shift due to nonlinear effect. We used microtremor records to estimate the natural period and damping of the building. By stacking sufficiently long records, we can extract those building property from microtremor records. Finally, the analysis models of the steel building used in the structure design are demonstrated based on the observed results. stories for the penthouse, and four stories under the ground level (Fig. 1(b)). The structure is steel moment-resisting frame above ground and steelreinforced concrete frame with shear walls under the ground level. The building is supported by the direct foundations with diaphragm walls. The depth of the direct foundation is 18 m from the ground and the diaphragm walls reaches to 35 m from the soil surface. The predominant natural periods of the design model are 2.28 seconds and 2.30 seconds in NS and EW direction, respectively (see Table 2). The designed damping factor is 2%.

Hypoxanthine increases basal and FGF-stimulated growth of porcine aortic endothelial cells in media with calf or newborn bovine serum.

Dear Editor: The growth of porcine aortic endothelial cells {PAEC) is known to require a serum supplement (5). However, we have usually experienced less PAEC growth-promoting activity by newborn bovine serum (NBS) or calf serum (CS) preparations, even when excess fibroblast growth factor (FGF} is present in the media. Thus, 10-20% of high quality fetal bovine serum (FBS) is generally used in our PAEC growth experiments. Very recently, we showed that hypoxanthine in FBS was essential for both basal and FGF-stimulated growth of PAEC in FBS containing media i3,4). In addition, it is reported that the hypoxanthine concentration is much lower in CS than it is in FBS (1). In this context, we thought that the deficiency of growth-promoting activities of NBS or CS might be due to the low levels of endogenous hypoxanthine, and if so, that the quality of the media containing them might be improved by the addition of exogenous hypoxanthine. In this study, we examined the relationship between the growth-promoting activity and hypoxanthine content of various bovine sera, and whether or not hypoxanthine

Desensitization by glycyrrhetinic acid of other stimulatory substance-induced increases in intracellular calcium in a variety of cell types

The effects of glycyrrhizin and its aglycone, glycyrrhetinic acid, on the cytoplasmic free calcium concentration ([Ca2+]i) in mastocytoma P-815 cells, DNP-Ascaris (antigen) sensitized mast cells, hepatocytes, fibroblasts and endothelial cells were investigated. In these cell types, glycyrrhetinic acid in the concentration range of 20 to 100 microM caused an increase in [Ca2+]i and inhibited calcium increases induced by an antigen (mast cells), ATP, phenylephrine (hepatocytes) and thrombin (fibroblasts and endothelial cells). Stimulation with phenylephrine, in place of glycyrrhetinic acid, did not inhibit subsequent calcium increases induced by phenylephrine or ATP. On the other hand, glycyrrhizin at concentrations up to 100 microM neither caused an increase in [Ca2+]i nor inhibited calcium increases induced by other stimulatory substances. These results suggest that the inhibition of the calcium-mediated signal pathway may participate in the cytostatic actions of glycyrrhetinic acid.

Regional Structural Investigation on the Preservation Districts of Yuasa and Ine in Japan

The preservation district of groups of traditional buildings is an important category of cultural properties in Japan. Japan has entered an earthquake active period. However, conventional culturally and socially oriented site investigation methods hardly satisfy the requirements of risk management of district preservation. In this study, the authors introduce an investigation method from the structural and security perspectives. They explain the study procedure, methods and results. Results of the investigation of two coastal districts, namely, Ine and Yuasa, are comparatively studied, and regional disparities are highlighted. The study concludes that district vulnerability and house structural performance should be considered as important indices when making decisions regarding district preservation.

Seismic performance evaluation on historical timber frame structures with large hanging walls

a) Specimens of which walls break before breakage of columns have high deformation performance. b) Different specification of hanging walls and size of column section give different failure mechanism and bearing force of hanging walls. c) Ratio of shear force of columns given by the results of tests is different from the ratio based on the current code. d) Bearing force of hanging walls is varied by specification of frames such as section size of columns and thickness of crosspieces called Nuki. e) Rear columns are broken at the bending unit stress which is about the half of the flexural strength.