Yasuji Kido

Formation of 12-oxo-trans-10-dodecenoic acid in chloroplasts from Thea sinensis leaves

Abstract Linolenic acid-[1- 14 C] was converted to 12-oxo- trans -10-dodecenoic acid, via 12-oxo- cis -9-dodecenoic acid by incubation with chloroplasts of Thea sinensis leaves. Thus, it was confirmed that linolenic acid is split into a C 12 -oxo-acid, 12-oxo- trans -10-dodecenoic acid, and a C 6 -aldehyde, trans -2-hexenal, leaf aldehyde, by an enzyme system in chloroplasts of tea leaves.

Effect of culture supernatant of MT-2 cells on human immunodeficiency virus-producing cells, MOLT-4/HIVHTLV-IIIB cells.

The effect of culture supernatant of MT‐2 cells on human immunodeficiency virus (HIV)‐producing cells, MOLT‐4/HIVHTLV‐IIIB cells, was examined. As compared to the effect on MOLT‐4 cells, parent cells not infected with HIV, a selective cytotoxic/cytostatic effect on MOLT‐4/ HIVHTLV‐IIIB cells was observed 4 days after treatment with up to 640‐fold‐diluted MT‐2 supernatant. Furthermore, under similar conditions, a 2‐ to 6‐fold increase in the number of HIV particles was detected in the culture of MOLT‐4/HIVHTLV‐IIIB cells 6 hr after treatment. Complete blocking of these effects by anti‐lymphotoxin monoclonal antibody, but not by anti‐tumor necrosis factor antibody, indicates that these effects of MT‐2 supernatant on MOLT‐4/HIVHTLV‐IIIB cells are attributable to a lymphotoxin‐related cytotoxic factor.

The Pleiotropic Properties of a Beta-Lactam Antibiotic-Hypersensitive Mutant Derived from Proteus vulgaris IFO 3167

It has been reported that most j3-lactam antibiotic-hypersensitive mutants from Escherichia coli (1), Pseudomonas aeruginosa (3), and Serratia marcescens (6) show pleiotropic properties that are considered to be due to cell surface membrane mutation. The authors found that a mutant from Proteus vulgaris strain also shows similar pleiotropic properties. In the present paper, the authors describe the isolation, sensitivity to various kinds of reagents, and changes of membrane components of a