Yasutomo Arashima

Peritonitis Associated With Pasteurella multocida: Molecular Evidence of Zoonotic Etiology

A patient on continuous cyclic peritoneal dialysis for chronic kidney disease due to type 2 diabetes mellitus developed peritoneal dialysis‐associated peritonitis induced by Pasteurella multocida that was isolated from a sample of dialysis effluent. The route of infection was unknown for this case; however, P. multocida was also isolated from a culture of a pharyngeal swab obtained from the patients cat. There was no evidence that the cat had bitten and ruptured the peritoneal dialysis tubing or bags. Pulsed‐field gel electrophoresis (PFGE) showed that the P. multocida isolated from the patient was completely identical to the strain isolated from the domestic cat. As there is a rise in the pet‐keeping population, an increase in zoonoses is to be expected. It is necessary to be carefully informed of hygiene rules in keeping pets because a pet may transmit zoonoses, even on casual contact.

A case of Pasteurella haemolytica sepsis in a patient with mitral valve disease who developed a splenic abscess

We report a patient with sepsis caused by Pasteurella haemolytica, an extremely rare etiologic agent of human infection, who had mitral valve disease and developed a splenic abscess.

A Study on Indoor Air Contaminants Related to Pets in Japanese Dwellings

Abstract The behavior of indoor particulate matter as it relates to pets was studied from the viewpoint of architectural hygiene. The viable particles from pets can be classified into two types, pathogenic microorganisms and pet allergens. This study investigated Pasteurella, which is a pathogenic organ of Pasteurellosis, dog allergen Can f1 and cat allergen Fel d1 in eight dwellings in Tokyo. In six of these dwellings (the case dwellings), the dogs and/or cats were allowed free access to indoor areas. Pasteurella and the pet allergens were quantified by biochemical and immunological methods, respectively. The relationship between suspended particles and airborne Fel d1 was also examined. Three species, P. canis, P. dagmatis and P. multocida, were isolated from the oral cavity of the dogs and cats. In the six case dwellings, dust allergen Can f1 and Fel d1 ranged from 1 to 1000 μg allergen/g dust and 100 to 5000 μg/g, respectively. The indoor air of the case dwellings contained concentrations of airborne Fel d1 ranging from 10 to 100 ng of allergen/m3 air. In addition, there was a significant relationship between the distributed concentration of suspended particles larger than 5 μm and airborne Fel d1 (P< 0.01). These findings suggest that the traditional reduction method for coarse particles, for example the use of a domestic air filtration cleaner, may be effective in removing airborne pet allergens.

Studies on growth of Pasteurella multocida on BTB agar

It has been reported that the Pasteurella multocida does not grow on the BTB agar. Therefore, this medium has been used as selective and differential medium for Pasteurella multocida. However, we have experienced that some of the Pasteurella multocida from the patients materials grew on the BTB agar. Here, we will report on the studies of the growth of the Pasteurella multocida strain on the BTB agar. Ten strains of Pasteurella multocida from humans and animals were used as the test strains. Those were adjusted to McFarland No. 5 by the sterilized physiological saline and inoculated on the agars. We compared commercially prepared BTB agars from 3 companies and BTB agars prepared by our-self from dehydrated culture medium. Blood, Chocolate, Nutrient and MacConkey agar were also used in this study. As for the growth of the Pasteurella multocida, we checked the pH of each agar and the temperature during the cultivation. The results are as follows: 1) Pasteurella multocida was confirmed to grow on all of the BTB agar. 2) Pasteurella multocida grew most heavily at 37 degrees C and pH of 7.4 to 8.2. 3) The difference of the growth on each agar was considered to be the difference of the pH and nutritional condition of the agar.