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Dive into the research topics where Yelda Özden Çiftçi is active.

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Featured researches published by Yelda Özden Çiftçi.


Plant Physiology and Biochemistry | 2016

Excess boron responsive regulations of antioxidative mechanism at physio-biochemical and molecular levels in Arabidopsis thaliana

Doğa Selin Kayıhan; Ceyhun Kayıhan; Yelda Özden Çiftçi

This work was aimed to evaluate the effect of boron (B) toxicity on oxidative damage level, non-enzymatic antioxidant accumulation such as anthocyanin, flavonoid and proline and expression levels of antioxidant enzymes including superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and glutathione reductase (GR) and their respective activities as well as expression levels of miR398 and miR408 in Arabidopsis thaliana. Plants were germinated and grown on MS medium containing 1 mM B (1B) and 3 mM B (3B) for 14 d. Toxic B led to a decrease of photosynthetic pigments and an increase in accumulation of total soluble and insoluble sugars in accordance with phenotypically viewed chlorosis of seedlings through increasing level of B concentration. Along with these inhibitions, a corresponding increase in contents of flavonoid, anthocyanin and proline occurred that provoked oxidative stress tolerance. 3B caused a remarkable increase in total SOD activity whereas the activities of APX, GR and CAT remained unchanged as verified by expected increase in H2O2 content. In contrast to GR, the coincidence was found between the expressions of SOD and APX genes and their respective activities. 1B induced mir398 expression, whereas 3B did not cause any significant change in expression of mir408 and mir398. Expression levels of GR genes were coordinately regulated with DHAR2 expression. Moreover, the changes in expression level of MDAR2 was in accordance with changes in APX6 expression and total APX activity, indicating fine-tuned regulation of ascorbate-glutathione cycle which might trigger antioxidative responses against B toxicity in Arabidopsis thaliana.


Archive | 2012

Novel Methods in Micropropagation of Pistachio

Engin Tilkat; Yelda Özden Çiftçi; Hülya Akdemir; Ahmet Onay; Emine Ayaz Tilkat

The focus of this paper is to describe the novel methods developed for the different stages of micropropagation: installation of mature apical shoot tips and elimination of browning exudates; forcing hardwood shoots from the lignified stem sections; forcing axenic leaves; initiation of embryogenic masses (EMS); encapsulation of somatic embryos and cryopreservation of axillary buds for storage, and the facilitation of rooting. These developed methods are not being used by commercial micropropagation laboratories yet. Exudation of phenolics is inhibited when the disinfested and rinsed explants are cut at the base of the shoot tips and washed twice for 1 h by shaking them in sterile distilled water on a shaker at 100 rpm. For shoot initiation, 15–20 cm long terminal stem sections are cut into 3–4 cm sections and placed in pots filled with peat, perlite and soil. Two or three weeks later, the developed softwood shoots are excised; or freshly harvested three to nine apical tips of 1–2 cm are disinfested and used as explants. Leaves excised from axenic shoot cultures were also used to induce organogenesis on a Murashige and Skoog (MS) medium with Gamborg vitamins supplemented with combinations of different concentrations of 6-benzylaminopurine (BA) and indole-3-asetic acid (IAA). Calcium alginate gel was used to encapsulate somatic embryos to produce synthetic seeds. The encapsulated somatic embryos can be stored under refrigerated conditions for short-term conservation. For long-term conservation, some axillary buds can also be stored by using vitrification and one-step freezing technique; however, other cryogenic techniques should also be tested. With these improved stages, application of Pistachio vera L. micropropagation in commercial clonal orchards will be feasible as an alternative to traditional propagation in the near future.


Archive | 2016

Cryopreservation of Somatic Embryos of Ornamental Plants

Emrah Kırdök; Hamit Ekinci; Yelda Özden Çiftçi

Ornamental plants play a social and economic role in human society since antic ages, and its production consists about 78 % of total production. Thus, in situ and ex situ germplasm conservation techniques must be applied to preserve elite varieties. Since in situ strategies are more prone to environmental factors (e.g., biotic and abiotic stress) and ex situ approaches are open to cross-pollination or homologous recombination during gamete formation, currently in vitro strategies are the good complementary mechanism to avoid these problems. Among in vitro conservation techniques, cryopreservation seems to be the best candidate as it enables to preserve selected germplasm theoretically unlimited period of time with maintaining genetic stability, which is very important in ornamental plant cultivation. Besides, embryogenic cultures are used for in vitro propagation; the tissues are also utilized as target materials for gene transfer studies. These cultures have the potential to produce cisgenic or intragenic plants and cryogenic technology offers opportunities to conserve germplasm to introduce genes from crossable ornamental plants especially for cisgenesis. Thus cryopreservation also plays an important role in maintaining transgenic, cisgenic, or intergenic somatic embryos of ornamentals in a stable way. In this chapter, efficient cryopreservation technique including one-step and two-step freezing methods together with vitrification- and dehydration-based techniques for conservation of somatic embryos and related tissues of ornamentals is discussed.


Plant Cell Tissue and Organ Culture | 2014

Micropropagation of the pistachio and its rootstocks by temporary immersion system

Hülya Akdemir; Veysel Süzerer; Ahmet Onay; Engin Tilkat; Yusuf Ersali; Yelda Özden Çiftçi


Plant Growth Regulation | 2015

Clonal micropropagation of Pistacia lentiscus L. and assessment of genetic stability using IRAP markers

F.M. Kilinç; Veysel Süzerer; Yelda Özden Çiftçi; Ahmet Onay; Hakan Yildirim; Ahu Altinkut Uncuoglu; Engin Tilkat; Ibrahim Koc; Ömer Faruk Akdemir; Özge Karakaş Metin


Journal of Plant Biochemistry and Biotechnology | 2013

In vitro conservation and cryopreservation of mature pistachio (Pistacia vera L.) germplasm

Hülya Akdemir; Veysel Süzerer; Engin Tilkat; Hakan Yildirim; Ahmet Onay; Yelda Özden Çiftçi


Applied Biochemistry and Biotechnology | 2016

Detection of Variation in Long-Term Micropropagated Mature Pistachio via DNA-Based Molecular Markers

Hülya Akdemir; Veysel Süzerer; Engin Tilkat; Ahmet Onay; Yelda Özden Çiftçi


Acta Physiologiae Plantarum | 2014

Cold-induced genetic instability in micropropagated Pistacia lentiscus L. plantlets

Ibrahim Koc; Hülya Akdemir; Ahmet Onay; Yelda Özden Çiftçi


Turkish Journal of Biology | 2014

In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.)

Ibrahim Koc; Ahmet Onay; Yelda Özden Çiftçi


Turkish Journal of Biology | 2016

Rejuvenation of mature lentisk by micrografting and evaluation of genetic stability

Ahmet Onay; Engin Tilkat; Veysel Süzerer; Özge Karakaş Metin; Yelda Özden Çiftçi; F.M. Kilinç; Ibrahim Koc; Muhammet Şakiroğlu; Hakan Yildirim; Ahu Altinkut Uncuoğlu; Nazan Çalar; Ömer Faruk Akdemir

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Hülya Akdemir

Gebze Institute of Technology

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Ibrahim Koc

Gebze Institute of Technology

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Doğa Selin Kayıhan

Gebze Institute of Technology

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