Yen-Yin Lin

Crystal growth of two-component new novel organic NLO crystals

Abstract Urea is an excellent nonlinear optical (NLO) medium. However, growth of large high-quality crystals for practical use still remains a challenge due to its unfavorable growth properties. Improved crystalline and optical quality could be expected in mixed systems. This work presents the synthesis, growth and second-harmonic-generation (SHG) studies of two-component new organic NLO systems, urea–meta nitro benzoic acid (mNBA) and urea– l -malic acid. The material property of urea seems to be modified by the addition of mNBA and l -malic acid. The SHG efficiency was measured by Kurtz powder method showing variation with composition variation.

Optogenetic control of selective neural activity in multiple freely moving Drosophila adults

Significance We present an automated laser tracking and optogenetic manipulation system (ALTOMS) for studying social memory in Drosophila. Based on behavioral interactions computed with a high-speed image analysis system, ALTOMS can target two lasers (a 473-nm blue laser and a 593.5-nm yellow laser) independently on any specified body parts of two freely moving Drosophila adults in real time. We performed an operant conditioning assay in which a courting male quickly learned and formed a long-lasting memory to stay away from a freely moving virgin female. Given its capacity for optogenetic manipulation to transiently and independently activate/inactivate selective neurons, ALTOMS offers opportunities to systematically map brain circuits that orchestrate specific Drosophila behaviors. We present an automated laser tracking and optogenetic manipulation system (ALTOMS) for studying social memory in fruit flies (Drosophila melanogaster). ALTOMS comprises an intelligent central control module for high-speed fly behavior analysis and feedback laser scanning (∼40 frames per second) for targeting two lasers (a 473-nm blue laser and a 593.5-nm yellow laser) independently on any specified body parts of two freely moving Drosophila adults. By using ALTOMS to monitor and compute the locations, orientations, wing postures, and relative distance between two flies in real time and using high-intensity laser irradiation as an aversive stimulus, this laser tracking system can be used for an operant conditioning assay in which a courting male quickly learns and forms a long-lasting memory to stay away from a freely moving virgin female. With the equipped lasers, channelrhodopsin-2 and/or halorhodopsin expressed in selected neurons can be triggered on the basis of interactive behaviors between two flies. Given its capacity for optogenetic manipulation to transiently and independently activate/inactivate selective neurons, ALTOMS offers opportunities to systematically map brain circuits that orchestrate specific Drosophila behaviors.

Broadband tunable optical parametric amplification from a single 50 MHz ultrafast fiber laser

We have demonstrated a 0.7 microm - 1.9 microm wavelength-tunable light source based on a single-pass optical parametric amplification (OPA) in a multiperiod magnesium oxide-doped periodically poled lithium niobate crystal. The OPA pump was a frequency-doubled ultrafast ytterbium-doped fiber oscillator, and the residual 1040 nm laser power after frequency doubling was recycled to generate a supercontinuum seeding source. Compared with conventional OPAs, this system is free from timing jitter between the pump laser and the seeding source. Over 50% conversion efficiency was obtained with 10 nJ pump energy. Combined with a 50 MHz repetition rate, this versatile source is ideal for biomedical and spectroscopic applications.

Pulsed optical parametric generation, amplification, and oscillation in monolithic periodically poled lithium niobate crystals

We conducted a series of passively Q-switched Nd:YAG laser pumped optical parametric generation, amplification, and oscillation experiments in monolithic periodically poled lithium niobate (PPLN) crystals. Double-pass optical parametric generation with an effective gain length of 10 cm in a PPLN crystal was performed in comparison with single-pass operation in the same crystal. By seeding a PPLN optical parametric amplifier with a distributed feedback (DFB) diode laser, we produced 200-ps transform-limited laser pulses at 1549.6 nm and observed parametric gain competition at different pump levels. For optical parametric oscillations, we first demonstrated 22% power efficiency from a 2.4-cm intrinsic-cavity PPLN optical parametric oscillator pumped by a 4.2-ns, 10-kW passively Q-switched Nd:YAG laser. Preliminary studies on DFB optical parametric oscillators in PPLN are mentioned. The temporal and spectral properties of these optical parametric generators, amplifiers, and oscillators are characterized and discussed.

Three‐dimensional structural imaging of starch granules by second‐harmonic generation circular dichroism

Chirality is one of the most fundamental and essential structural properties of biological molecules. Many important biological molecules including amino acids and polysaccharides are intrinsically chiral. Conventionally, chiral species can be distinguished by interaction with circularly polarized light, and circular dichroism is one of the best‐known approaches for chirality detection. As a linear optical process, circular dichroism suffers from very low signal contrast and lack of spatial resolution in the axial direction. It has been demonstrated that by incorporating nonlinear interaction with circularly polarized excitation, second‐harmonic generation circular dichroism can provide much higher signal contrast. However, previous circular dichroism and second‐harmonic generation circular dichroism studies are mostly limited to probe chiralities at surfaces and interfaces. It is known that second‐harmonic generation, as a second‐order nonlinear optical effect, provides excellent optical sectioning capability when combined with a laser‐scanning microscope. In this work, we combine the axial resolving power of second‐harmonic generation and chiral sensitivity of second‐harmonic generation circular dichroism to realize three‐dimensional chiral detection in biological tissues. Within the point spread function of a tight focus, second‐harmonic generation circular dichroism could arise from the macroscopic supramolecular packing as well as the microscopic intramolecular chirality, so our aim is to clarify the origins of second‐harmonic generation circular dichroism response in complicated three‐dimensional biological systems.

Thermal waveguide OPO

We report a mid-infrared, CW singly resonant optical parametric oscillator (OPO) with a thermally induced waveguide in its gain crystal. We measured a numerical aperture of 0.0062 for the waveguide at 80-W intracavity power at 3.2 microm. This thermal-guiding effect benefits to the stable operation of an OPO and improves the parametric conversion efficiency by more than a factor of two when compared with that without thermal guiding.

Non-invasive manipulation of Drosophila behavior by two-photon excited red-activatable channelrhodopsin

Scattering and absorption limit light penetration through inhomogeneous tissue. To reduce scattering, biochemists have shifted the wavelengths of excitation light for optogenetic actuators and fluorescent proteins to the orange-red range, while physicists have developed multiphoton technologies for deep tissue stimulation. We have built a rapid multiphoton spectroscopic screening system of genetically encoded red-activatable channelrhodopsin (ReaChR), and considered specific behaviors in transgenic Drosophila melanogaster as readouts to optimize the laser parameters for two-photon optogenetic activation. A wavelength-tunable optical parametric amplifier was adopted as the major light source for widefield two-photon excitation (TPE) of ReaChR. Our assays suggest that the optimized TPE wavelength of ReaChR is 1250 nm. Exploiting its capacity for optogenetic manipulation to induce macroscopic behavioral change, we realized rapid spectroscopic screening of genetically encoded effectors or indicators in vivo, and used modulation of ReaChR in the fly as a successful demonstration of such a system.

High power broadband continuum source based on an all-PM-fiber master oscillator nonlinear power amplifier

In an all-polarization-maintaining-fiber master oscillator power amplifier system at 1064 nm under all normal dispersion, intense nanosecond emission was generated with spectral broadening from 980 to 1600 nm. In such a fiber nonlinear power amplifier, efficient power scaling is able to be free from significant depletion because both laser amplification and nonlinear conversion are simultaneously employed. As a result, output peak power up to 117 kW with a pulse energy of 1.2 mJ is generated with a maximum core intensity of 30 GW cm 2 . In addition, the conversion efficiency is 66% for a pulse duration of 6.1 ns at the moderate repetition of 20 kHz. The output level is close to the damage threshold for long-term operation. The onset and interplay of constituted fiber nonlinearities can be addressed, especially from single mode to a few modes, stage by stage. Furthermore, the seeding influence on the spectral broadening reveals its versatility for enabling many potential applications. For seeding by a highly controlled diode laser at the nanojoule level, a double-pass preamplifier significantly improves the energy extraction, resulting in a high input level for an efficient nonlinear power amplifier. Such a linearly polarized light source composed of an intense 1064 nm pump and a broad sideband seed is beneficial for efficiently driving broadband tunable optical parametric amplification.

Long-range parametric amplification of THz wave with absorption loss exceeding parametric gain.

Optical parametric mixing is a popular scheme to generate an idler wave at THz frequencies, although the THz wave is often absorbing in the nonlinear optical material. It is widely suggested that the useful material length for co-directional parametric mixing with strong THz-wave absorption is comparable to the THz-wave absorption length in the material. Here we show that, even in the limit of the absorption loss exceeding parametric gain, the THz idler wave can grows monotonically from optical parametric amplification over a much longer distance in a nonlinear optical material until pump depletion. The coherent production of the non-absorbing signal wave can assist the growth of the highly absorbing idler wave. We also show that, for the case of an equal input pump and signal in difference frequency generation, the quick saturation of the THz idler wave predicted from a much simplified and yet popular plane-wave model fails when fast diffraction of the THz wave from the co-propagating optical mixing waves is considered.

Three-wavelength light control of freely moving Drosophila Melanogaster for less perturbation and efficient social-behavioral studies.

We developed a real-time automated laser-tracking system combined with continuous wave 1064-nm infrared or 473-nm blue lasers to provide punishment for studying memory in Drosophila Melanogaster. Combining optogenetic tools with laser properties, such as 473-nm and 593-nm lasers that activate light sensitive proteins in artificial transgenic flies, we can manipulate the specific neuron of an assigned fly among multiple flies to investigate neuron circuit relationships in social interactions. In restraining condition assay or optogenetic experiments, a ventral irradiated system would be more efficient due to higher ventral cuticle transmissions and neuron ganglia locations. Therefore, ventral irradiated systems cause less perturbation during behavior studies.