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Featured researches published by Yijuan Ding.


European Journal of Plant Pathology | 2012

Screening resistance against Sclerotinia sclerotiorum in Brassica crops with use of detached stem assay under controlled environment

Jiaqin Mei; Dayong Wei; Joseph Onwusemu Disi; Yijuan Ding; Yao Liu; Wei Qian

Sclerotinia sclerotiorum is a major pathogen that infects stem tissue, causing yield loss and poor seed quality in rapeseed (Brassica napus). Here we report a reliable detached stem inoculation method for evaluating resistance under controlled environment. Two sets of Brassica materials were employed, including 17 genotypes from five Brassica crops in experiment 1, and 71 F2 lines derived from a cross between susceptible and resistant lines of B. oleracea in experiment 2. High correlations were detected between stem and branch for lesion length in both experiments and between stem and sections of stem in experiment 1. Although the lesion length of detached stem inoculation under controlled environment was positively correlated with that of toothpick inoculation in the field, the variation of lesion length in the detached stem inoculation was lower than that in toothpick inoculation in experiment 2. Moreover, no significant association was detected between lesion length and the diameter of stem or branch. These findings suggest that the detached stem inoculation under controlled conditions is a large-scale, flexible and reliable method of screening for resistance against S. sclerotiorum in Brassica crops. The application of detached stem inoculation is discussed in resistance breeding programs against S. sclerotiorum in Brassica crops.


Journal of Experimental Botany | 2017

A genome-wide survey with different rapeseed ecotypes uncovers footprints of domestication and breeding

Dayong Wei; Yixin Cui; Yajun He; Qing Xiong; Lunwen Qian; Chaobo Tong; Guangyuan Lu; Yijuan Ding; Jiana Li; Christian Jung; Wei Qian

In 198 selective sweeps we found functional genes or loci, such as FT and resistance, associated with environmental adaptability and yield-related traits.


Frontiers in Plant Science | 2017

Time-Series Analyses of Transcriptomes and Proteomes Reveal Molecular Networks Underlying Oil Accumulation in Canola

Huafang Wan; Yixin Cui; Yijuan Ding; Jiaqin Mei; Hongli Dong; Wenxin Zhang; Shiqi Wu; Ying Liang; Chunyu Zhang; Jiana Li; Qing Xiong; Wei Qian

Understanding the regulation of lipid metabolism is vital for genetic engineering of canola (Brassica napus L.) to increase oil yield or modify oil composition. We conducted time-series analyses of transcriptomes and proteomes to uncover the molecular networks associated with oil accumulation and dynamic changes in these networks in canola. The expression levels of genes and proteins were measured at 2, 4, 6, and 8 weeks after pollination (WAP). Our results show that the biosynthesis of fatty acids is a dominant cellular process from 2 to 6 WAP, while the degradation mainly happens after 6 WAP. We found that genes in almost every node of fatty acid synthesis pathway were significantly up-regulated during oil accumulation. Moreover, significant expression changes of two genes, acetyl-CoA carboxylase and acyl-ACP desaturase, were detected on both transcriptomic and proteomic levels. We confirmed the temporal expression patterns revealed by the transcriptomic analyses using quantitative real-time PCR experiments. The gene set association analysis show that the biosynthesis of fatty acids and unsaturated fatty acids are the most significant biological processes from 2-4 WAP and 4-6 WAP, respectively, which is consistent with the results of time-series analyses. These results not only provide insight into the mechanisms underlying lipid metabolism, but also reveal novel candidate genes that are worth further investigation for their values in the genetic engineering of canola.


Theoretical and Applied Genetics | 2013

Identification of genomic regions involved in resistance against Sclerotinia sclerotiorum from wild Brassica oleracea

Jiaqin Mei; Yijuan Ding; Kun Lu; Dayong Wei; Yao Liu; Joseph Onwusemu Disi; Jiana Li; Liezhao Liu; Shengyi Liu; John K. McKay; Wei Qian


Theoretical and Applied Genetics | 2015

Transfer of sclerotinia resistance from wild relative of Brassica oleracea into Brassica napus using a hexaploidy step

Jiaqin Mei; Yao Liu; Dayong Wei; Benjamin Wittkop; Yijuan Ding; Qinfei Li; Jiana Li; Huafang Wan; Zaiyun Li; Xianhong Ge; Martin Frauen; Rod J. Snowdon; Wei Qian; Wolfgang Friedt


Genetic Resources and Crop Evolution | 2013

Improvement of Sclerotinia sclerotiorum resistance in Brassica napus by using B. oleracea

Yijuan Ding; Jiaqin Mei; Qinfei Li; Yao Liu; Huafang Wan; Lei Wang; Heiko C. Becker; Wei Qian


The Journal of Agricultural Science | 2014

Inheritance of leaf and stem resistance to Sclerotinia sclerotiorum in a cross between Brassica incana and Brassica oleracea var. alboglabra

Joseph Onwusemu Disi; Jiaqin Mei; Dayong Wei; Yijuan Ding; Wei Qian


Molecular Breeding | 2015

Transfer of sclerotinia stem rot resistance from wild Brassica oleracea into B. rapa

Yijuan Ding; Jiaqin Mei; Yao Liu; Lei Wang; Yuehua Li; Huafang Wan; Jiana Li; Wei Qian


Phytopathology | 2018

Simultaneous transcriptome analysis of host and pathogen highlights the interaction between Brassica oleracea and Sclerotinia sclerotiorum

Yijuan Ding; Jiaqin Mei; Yaru Chai; Yang Yu; Chaoguo Shao; Qinan Wu; Joseph Onwusemu Disi; Yuhua Li; Huafang Wan; Wei Qian


Journal of Plant Pathology | 2017

EXPLORING GENE INDICATORS TO PREDICT SUSCEPTIBILITY TO SCLEROTINIA SCLEROTIORUM IN BRASSICA PLANTS

Yuehua Li; Yijuan Ding; Chaoguo Shao; Jiaqin Mei; Wei Qian

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Wei Qian

Southwest University

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Jiana Li

Southwest University

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Yao Liu

Southwest University

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