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Dive into the research topics where Yingyos Jittayasothorn is active.

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Featured researches published by Yingyos Jittayasothorn.


Immunity | 2015

Microbiota-Dependent Activation of an Autoreactive T Cell Receptor Provokes Autoimmunity in an Immunologically Privileged Site

Reiko Horai; Carlos Zárate-Bladés; Patricia Dillenburg-Pilla; Jun Chen; Jennifer L. Kielczewski; Phyllis B. Silver; Yingyos Jittayasothorn; Chi-Chao Chan; Hidehiro Yamane; Kenya Honda; Rachel R. Caspi

Activated retina-specific T cells that have acquired the ability to break through the blood-retinal barrier are thought to be causally involved in autoimmune uveitis, a major cause of human blindness. It is unclear where these autoreactive T cells first become activated, given that their cognate antigens are sequestered within the immune-privileged eye. We demonstrate in a novel mouse model of spontaneous uveitis that activation of retina-specific T cells is dependent on gut commensal microbiota. Retina-specific T cell activation involved signaling through the autoreactive T cell receptor (TCR) in response to non-cognate antigen in the intestine and was independent of the endogenous retinal autoantigen. Our findings not only have implications for the etiology of human uveitis, but also raise the possibility that activation of autoreactive TCRs by commensal microbes might be a more common trigger of autoimmune diseases than is currently appreciated.


Journal of Immunology | 2015

Retina-Specific T Regulatory Cells Bring About Resolution and Maintain Remission of Autoimmune Uveitis

Phyllis B. Silver; Reiko Horai; Jun Chen; Yingyos Jittayasothorn; Chi-Chao Chan; Rafael Villasmil; Muge R. Kesen; Rachel R. Caspi

Experimental autoimmune uveitis (EAU) induced in mice by immunization with the retinal Ag interphotoreceptor retinoid-binding protein (IRBP) is a model of human autoimmune uveitis. We examined whether T regulatory cells (Tregs) found in uveitic eyes are IRBP specific, functionally suppressive, and play a role in natural resolution of disease and in maintenance of remission. Progressive increase of Foxp3+ Treg to T effector cell (Teff) ratio in uveitic eyes correlated with resolution of disease. At peak disease, up to 20% of Tregs (CD4+Foxp3+) and up to 60% of Teffs (CD4+Foxp3−) were IRBP specific, whereas in lymphoid organs retina-specific T cells were undetectable. Tregs isolated from eyes of mice with EAU efficiently suppressed IRBP-specific responses of Teffs from the same eyes. Importantly, systemic depletion of Tregs at peak disease delayed resolution of EAU, and their depletion after resolution triggered a relapse. This could be partially duplicated by depletion of Tregs locally within the eye. Thus, the T cell infiltrate in uveitic eyes of normal mice with a polyclonal T cell repertoire is highly enriched in IRBP-specific Tregs and Teffs. Unlike what has been reported for Tregs in other inflammatory sites, Tregs from uveitic eyes appear unimpaired functionally. Finally, Foxp3+ Tregs play a role in the natural resolution of uveitis and in the maintenance of remission, which occurs at least in part through an effect that is local to the eye.


Journal of Experimental Medicine | 2015

NK-DC crosstalk controls the autopathogenic Th17 response through an innate IFN-γ–IL-27 axis

Wai Po Chong; Nicholas van Panhuys; Jun Chen; Phyllis B. Silver; Yingyos Jittayasothorn; Mary J. Mattapallil; Ronald N. Germain; Rachel R. Caspi

DCs recruit NK cells to the draining lymph node where they interact with DC creating a positive feedback loop of IL-27 and IFNγ production, which is ultimately limited by IL-10. This innate NK-DC axis controls the development of the adaptive response and dampens induction of autoimmunity.


Journal of Immunology | 2016

Tertiary Lymphoid Tissue Forms in Retinas of Mice with Spontaneous Autoimmune Uveitis and Has Consequences on Visual Function

Jennifer L. Kielczewski; Reiko Horai; Yingyos Jittayasothorn; Chi-Chao Chan; Rachel R. Caspi

During chronic inflammation, tertiary lymphoid tissue (TLT) can form within an inflamed organ, including the CNS. However, little is known about TLT formation in the neuroretina. In a novel spontaneous autoimmune mouse model of uveitis (R161H), we identified well-organized lymphoid aggregates in the retina and examined them for TLT characteristics. Presence of immune cells, tissue-specific markers, and gene expression patterns typically associated with germinal centers and T follicular helper cells were examined using immunohistochemistry and gene analysis of laser capture microdissected retina. Our data revealed the retinal lymphoid structures contained CD4+ T cells and B cells in well-defined zonal areas that expressed classic germinal center markers, peanut lectin (agglutinin) and GL-7. Gene expression analysis showed upregulation of T follicular helper cell markers, most notably CXCR5 and its ligand CXCL13, and immunohistochemical analysis confirmed CXCR5 expression, typically associated with CD4+ T follicular helper cells. Highly organized stromal cell networks, a hallmark of organized lymphoid tissue, were also present. Positive staining for phospho-Zap70 in retina-specific T cells indicated CD4+ T cells were being activated within these lymphoid structures. CD138+/B220+ plasma cells were detected, suggesting the retinal lymphoid aggregates give rise to functional germinal centers, which produce Abs. Interestingly, eyes with lymphoid aggregates exhibited lower inflammatory scores by fundus examination and a slower initial rate of loss of visual function by electroretinography, compared with eyes without these structures. Our findings suggest that the lymphoid aggregates in the retina of R161H mice represent organized TLT, which impact the course of chronic uveitis.


Investigative Ophthalmology & Visual Science | 2015

Characterization of a New Epitope of IRBP That Induces Moderate to Severe Uveoretinitis in Mice With H-2b Haplotype.

Mary J. Mattapallil; Phyllis B. Silver; Lizette M. Cortes; Anthony J. St. Leger; Yingyos Jittayasothorn; Jennifer L. Kielczewski; James J. Moon; Chi-Chao Chan; Rachel R. Caspi

PURPOSE Experimental autoimmune uveitis (EAU) induced in mice using the retinal antigen interphotoreceptor retinoid binding protein (IRBP) is an animal model for posterior uveitis in humans. However, EAU induced by native IRBP protein or its widely used epitope amino acid residues 1 to 20 of human IRBP (hIRBP1-20) is inconsistent, often showing low scores and incidence. We found an urgent need to identify a better pathogenic epitope for the C57BL/6 strain. METHODS Mice were immunized with uveitogenic peptides or with native bovine IRBP. Clinical and histological disease and associated immunological responses were evaluated. Truncated and substituted peptides, as well as bioinformatic analyses, were used to identify critical major histocompatibility complex (MHC)/T cell receptor (TCR) contact residues and the minimal core epitope. RESULTS The new uveitogenic epitope of IRBP, amino acid residues 651 to 670 of human IRBP (LAQGAYRTAVDLESLASQLT [hIRBP651-670]) is uveitogenic for mice of the H-2b haplotype and elicits EAU with a higher severity and incidence in C57BL/6 mice than the previously characterized hIRBP1-20 epitope. Using truncated and substituted peptides, as well as bioinformatic analysis, we identified the critical contact residues with MHC/TCR and defined the minimal core epitope. This made it possible to design MHC tetramers and use them to detect epitope-specific T cells in the uveitic eye and in lymphoid organs of hIRBP651-670-immunized mice. CONCLUSIONS Data suggest that hIRBP651-670 is an epitope naturally processed from a conserved region of native IRBP, potentially explaining its relatively high uveitogenicity. This epitope should be useful for basic and preclinical studies of uveitis in the C57BL/6 model and gives access to genetically engineered mice available on this background.


Journal of Autoimmunity | 2013

Breakdown of immune privilege and spontaneous autoimmunity in mice expressing a transgenic T cell receptor specific for a retinal autoantigen

Reiko Horai; Phyllis B. Silver; Jun Chen; Rajeev K. Agarwal; Wai Po Chong; Yingyos Jittayasothorn; Mary J. Mattapallil; Sonia Nguyen; Kannan Natarajan; Rafael Villasmil; Peng Wang; Zaruhi Karabekian; Simon D. Lytton; Chi-Chao Chan; Rachel R. Caspi


Investigative Ophthalmology & Visual Science | 2014

Activation of Autoreactive T Cells by Endogenous Commensal Microflora Provokes Spontaneous Autoimmunity in the Immunologically Privileged Eye

Rachel R. Caspi; Reiko Horai; Carlos Zárate-Bladés; Phyllis B. Silver; Jun Chen; Yingyos Jittayasothorn; Hidehiro Yamane; Patricia Dillenburg-Pilla; Chi-Chao Chan; Kenya Honda


Investigative Ophthalmology & Visual Science | 2017

Neuroprotective effects of IL-22 during CNS inflammation

Rachel R. Caspi; Rachael C. Rigden; Jennifer L. Kielczewski; Carlos Zárate-Bladés; Anthony J. St. Leger; Phyllis B. Silver; Yingyos Jittayasothorn; Chi-Chao Chan; Mary J. Mattapallil


Investigative Ophthalmology & Visual Science | 2017

Vitamin A deficiency impacts acquisition, but not expression, of autoimmunity to the neuroretina

Reiko Horai; Ru Zhou; So Jin Bing; Kaska Wloka; Jun Chen; Phyllis B. Silver; Yingyos Jittayasothorn; Rachel R. Caspi


Investigative Ophthalmology & Visual Science | 2017

IL-17A inhibits expression of IL-17-ineage cytokines through a negative feedback loop involving IL-24 and controls autoimmune uveitis

Kumarkrishna Raychaudhuri; Wai Po Chong; Reiko Horai; Phyllis B. Silver; Yingyos Jittayasothorn; Chi-Chao Chan; Jun Chen; Rachel R. Caspi

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Rachel R. Caspi

National Institutes of Health

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Phyllis B. Silver

National Institutes of Health

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Chi-Chao Chan

National Institutes of Health

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Reiko Horai

National Institutes of Health

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Jun Chen

National Institutes of Health

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Wai Po Chong

National Institutes of Health

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Mary J. Mattapallil

National Institutes of Health

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Anthony J. St. Leger

National Institutes of Health

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