Yohei Watanabe
Fukushima Medical University
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Featured researches published by Yohei Watanabe.
Oncology Letters | 2017
Yoshiko Matsumoto; Motonobu Saito; Katsuharu Saito; Yasuyuki Kanke; Yohei Watanabe; Hisashi Onozawa; Suguru Hayase; Wataru Sakamoto; Teruhide Ishigame; Tomoyuki Momma; Kensuke Kumamoto; Shinji Ohki; Seiichi Takenoshita
Kinesin family member 4A (KIF4A) is a member of the kinesin 4 subfamily of kinesin-related proteins and serves an important role in cell division. The expression levels of KIF4A have been investigated in numerous types of cancer, including cervical, lung, oral, and breast cancer, and are established to be associated with poor patient prognosis. However, the role of KIF4A, as well as its expression in colorectal cancer (CRC), remains to be elucidated. Therefore, the current study investigated KIF4A expression levels in patients with CRC and demonstrated that its levels were increased in tumor tissues compared with non-tumor tissues. To investigate the functional role of KIF4A, KIF4A was knocked down in CRC cells and cell viability was evaluated. CRC cells with KIF4A knockdown exhibited lower cell proliferation compared with control cells. In addition, KIF4A expression levels, as determined by immunohistochemistry, were compared with the expression of Ki-67, but no significant associations were observed in the patients with CRC. Therefore, KIF4A was found to be upregulated in patients with CRC and downregulation of KIF4A reduced cell proliferation in CRC cells. These results suggest that KIF4A may be a potential therapeutic target, which may improve the outcomes of patients with CRC.
Oncology Letters | 2017
Daiki Kikuchi; Motonobu Saito; Katsuharu Saito; Yohei Watanabe; Yoshiko Matsumoto; Yasuyuki Kanke; Hisashi Onozawa; Suguru Hayase; Wataru Sakamoto; Teruhide Ishigame; Tomoyuki Momma; Shinji Ohki; Seiichi Takenoshita
Solute carrier (SLC) drug transporters exchange various molecules without energy from adenosine triphosphate hydrolysis, indicating an association with anticancer drug resistance. However, the expression and role of SLC transporters in malignant tumors has not yet been fully elucidated. Therefore, in the current study, the expression of SLC37A family genes was evaluated in patients with colorectal cancer (CRC), and it was revealed that SLC family 37 member 1 (SLC37A1) expression was significantly increased in tumorous tissues compared with that in non-tumorous tissues. The cases with upregulated expression of SLC37A1 by immunohistochemical staining were significantly associated with positive venous invasion and liver metastasis. Furthermore, upregulated SLC37A1 expression was associated with poor overall survival time in the present cohort. These results indicated that SLC37A1 is involved in the hematogenous metastasis of CRC. To investigate whether SLC37A1 is associated with hematogenous metastasis and glycolipid metabolism, SLC37A1 was knocked down in colon cancer cells, and the expression of sialyl Lewis A and sialyl Lewis X was observed to be decreased. In summary, upregulation of SLC37A1 was observed in patients with CRC, and was associated with poor patient outcomes and survival. To the best of our knowledge, the present study is the first to propose a key role of SLC37A1 in CRC, and additional studies are warranted to reveal the functional role of SLC37A1 in CRC development.
Oncology Letters | 2017
Yohei Watanabe; Motonobu Saito; Katsuharu Saito; Yoshiko Matsumoto; Yasuyuki Kanke; Hisashi Onozawa; Suguru Hayase; Wataru Sakamoto; Teruhide Ishigame; Tomoyuki Momma; Shinji Ohki; Seiichi Takenoshita
Homeobox A (HOXA) cluster genes, members of the HOX family, perform an important role in normal organ development. It has previously been reported that HOXA gene expression in various types of cancer is associated with poor patient outcomes. However, the role of HOXA genes, as well as their expression, in colorectal cancers (CRC) remains unknown. Therefore, the present study investigated HOXA gene expression in patients with CRC and revealed that HOXA9 expression was significantly increased in tumor tissues compared with non-tumor tissues. Additionally, the functional role of HOXA9 was assessed by knocking down the HOXA9 gene in CRC cells and by evaluating cell growth. Regarding gene expression, cases with positive HOXA9 expression (as detected by immunohistochemical staining) were significantly associated with higher TNM stage and positive lymph node metastasis, although no association was observed between increased HOXA9 levels and the rate of overall survival in the present cohort. Regarding the functional role, HOXA9 expression was demonstrated to be upregulated in patients with CRC and was associated with lymph node metastasis.
Fukushima journal of medical science | 2014
Satoshi Suzuki; Yohei Watanabe; Takashi Yazawa; Teruhide Ishigame; Motoki Sassa; Tomoyuki Monma; Tadashi Takawa; Kensuke Kumamoto; Izumi Nakamura; Shinji Ohoki; Yuichi Hatakeyama; Hiroshi Sakuma; Toshiyuki Ono; Sadao Omata; Seiichi Takenoshita
BACKGROUND/AIMS We examined whether conventional ultrasonography (US) and computed tomography (CT) were useful to evaluate liver hardness and hepatic fibrosis by comparing the results with those obtained by a tactile sensor using rats with liver fibrosis. METHODOLOGY We used 44 Wistar rats in which liver fibrosis was induced by intraperitoneal administration of thioacetamide. The CT and US values of each liver were measured before laparotomy. After laparotomy, a tactile sensor was used to measure liver hardness. We prepared Azan stained sections of each excised liver specimen and calculated the degree of liver fibrosis (HFI: hepatic fibrosis index) by computed color image analysis. RESULTS The stiffness values and HFI showed a positive correlation (r=0.690, p<0.001), as did the tactile values and HFI (r=0.709, p<0.001).In addition, the stiffness and tactile values correlated positively with each other (r=0.814, p<0.001). There was no correlation between the CT values and HFI, as well as no correlation between the US values and HFI. CONCLUSION We confirmed that it was difficult to evaluate liver hardness and HFI by CT or US examination, and considered that, at present, a tactile sensor is useful method for evaluating HFI.
Nippon Daicho Komonbyo Gakkai Zasshi | 2013
Tomoyuki Momma; Daiki Kikuchi; Yohei Watanabe; Hisashi Onozawa; Satoshi Suzuki; Izumi Nakamura; Shinji Ohki; Noriyuki Yoshida; Seiichi Takenoshita
The Japanese Journal of Gastroenterological Surgery | 2010
Tomoyuki Monma; Yohei Watanabe; Takeshi Sakuma; Masami Matsuzaki; Naoto Katagata; Fumiaki Watanabe; Tadashi Nomizu; Seiichi Takenoshita
Oncology Reports | 2017
Hisashi Onozawa; Motonobu Saito; Katsuharu Saito; Yasuyuki Kanke; Yohei Watanabe; Suguru Hayase; Wataru Sakamoto; Teruhide Ishigame; Tomoyuki Momma; Shinji Ohki; Seiichi Takenoshita
Nihon Rinsho Geka Gakkai Zasshi (journal of Japan Surgical Association) | 2013
Yohei Watanabe; Yusuke Kobayashi; Daiki Kikuchi; Koichi Nakayama; Kojiro Urazumi; Seiichi Takenoshita
The Japanese Biochemical Society/The Molecular Biology Society of Japan | 2017
saori sugita; Kumiko Watanabe; Kana Hashimoto; Yohei Watanabe
Nihon Rinsho Geka Gakkai Zasshi (journal of Japan Surgical Association) | 2014
Yohei Watanabe; Yasuhide Kofunato; Takashi Yazawa; Yoshimasa Ishii; Seiichi Takenoshita