Yoji Takei

The Distribution of 2,4‐Dinitrophenyl Groups on Thy‐1 Positive Cells in the Epidermis of Mouse Following Skin Painting with 2,4‐Dinitrochlorobenzene

The distribution of 2,4‐dinitrophenyl (DNP) groups on Thy‐1 positive epidermal cells in vivo after 2,4‐dinitrochlorobenzene (DNCB) was painted on the skin surface of C3H/He mice was examined. Epidermal cell suspensions (EC) derived from trypsin‐separated mouse ear skin painted with DNCB were double‐stained for DNP groups and Thy‐1 protein using FITC‐anti‐DNP antibody, biotinylated anti‐mouse Thy‐1,2 monoclonal antibody, and phyco‐erythrin conjugated avidin. There were on the average 1.7% Thy‐1+ ECs; DNP groups were detectable on 77.8% of them when the specimen was treated with anti‐DNP followed by anti‐Thy‐1,2 incubation. The incidence of Thy‐1,2+ EC with DNP groups decreased when cells were treated with these antibodies in the reverse order. The significance of these findings is discussed in relationship to the mode of DNCB Thy‐1+ EC binding.

Delayed-type skin reaction to 2,4-dinitrophenylated epidermal cells in guinea pigs with contact sensitivity to 2,4-dinitrochlorobenzene

SummaryContact sensitivity (CS) induced by hapten has been thought to be analogous to delayed-type hypersensitivity, such as the Mantoux reaction, because of outstanding similarities between the two phenomena. It can be suggested that animals with CS respond also to intradermal injection of the conjugate of hapten and protein as well as to epicutaneous application of hapten. However, evidence against this has been reported. In the present experiments, delayed-type skin reaction (DSR) was successfully obtained in JY1 strain guinea pigs sensitized by painting the skin with 2,4-dinitrochlorobenzene using in vitro dinitrophenylated epidermal cell suspension (DNP-EC) as antigen for a delayed intradermal test. The experiment using anti-Ia alloantiserum and complement showed that the elicitation of DSR is due to the presence of Ia-positive cells (presumably Langerhans cells) among DNP-ECs. The delayed intradermal test with the conjugates such as haptenated ECs in the animals with CS is considered to be an experimentally useful way of analysing the antigen in the sensitivity.

Histological study on the fate of haptenated epidermal cells injected intradermally in guinea pigs

SummaryThe percutaneous administration of in vitro haptenated epidermal cells (EC) has become established as a procedure to produce contact sensitivity (CS) in experimental animals for routine use. The cells have also been found to elicit a significant delayed-type skin reaction by intradermal test in the animals sensitized by painting the skin with the hapten. The fate of 2,4-dinitrophenylated (DNP) isogeneic epidermal cell suspensions (EC) injected intradermally was investigated histologically in intact or 2,4-dinitrochlorobenzene (DNCB)-sensitized strain 13 guinea pigs to study the role of the cells in CS. DNP-EC were found to proliferate actively in the dermis and formed EC nests with central keratinization and then elicited inflammatory reaction associated with necrosis of the epidermal structures 7 days after injection in the intact animals. DNP-EC injected intradermally into the animals which had received and reacted against DNCB underwent a suppression of EC proliferation. These findings are discussed in relation to the role of the haptenated EC in CS.

Therapeutic indication for ingrown nail.

130例, 234患部の陥入爪を治療した。治療成績は, 手術療法(爪甲を含めた爪一部切除術)では94.7%が完治し, 保存療法(爪甲先端の角を爪廓皮膚よりのばす)では40.8%が完治し, 抜爪では大部分が再発していた。最終的に行つた治療方法と, 重症度, 罹患期間, 抜爪歴の有無の相関を調べ, 治療適応を検討した。(1)軽症で罹患期間の短いものは保存療法をする, (2)中等症, 重症でも抜爪歴がなく, 爪甲の変形が軽度のものは保存療法を試み, 2週間程度で軽快傾向のないものは手術療法を勧める, (3)抜爪歴を有し, 爪甲の変形が強いものは早期に手術療法を施行する, という方針が得られた。抜爪や爪切りでは, ほとんど再発し, むしろ悪化する傾向があるので, 施行すべきではないと考えた。

The distribution of 2,4-dinitrophenyl groups in guinea pigs following feeding of 2,4-dinitrochlorobenzene.

SummaryAn immunofluorescent technique using anti-DNP antibody was employed to investigate the distribution of 2,4-dinitrophenyl (DNP) groups in various tissues following the feeding of 125 mg/kg 2,4-dinitrochlorobenzene (DNCB)-ethanol to guinea pigs that had been starved for 1 day. DNP groups were detected in the areas corresponding to the cytoplasm and the cell membrane of the epithelium in the upper gastrointestinal tract as well as on the cells of mesenteric lymph nodes, Payers patches, the spleen, and peripheral blood. These results are discussed in relation to the mechanism of tolerance induction produced by the feeding of haptens.