Yoko Hoshi

Detection of dynamic changes in cerebral oxygenation coupled to neuronal function during mental work in man

Changes in the oxygenation state of brain hemoglobin during mental work were monitored in real time (time resolution: 1 s) using near-infrared spectrophotometry in 14 healthy volunteers. In a younger group (age range 22-30 years, n = 12), the subjects finding difficulty in solving a problem were accompanied by significant increases in both oxygenated hemoglobin and total hemoglobin, while the subjects making a correct answer without difficulty showed no significant changes in hemoglobin oxygenation. When compared with those younger subjects, observations on the older subject demonstrated a decrease in oxygenated hemoglobin and a reciprocal increase in deoxygenated hemoglobin, unaccompanied by changes in total hemoglobin.

Visuospatial imagery is a fruitful strategy for the digit span backward task: A study with near-infrared optical tomography.

Our newly developed 64-channel time-resolved optical tomographic imaging system using near-infrared light enables us to obtain a quantitative image of hemoglobin concentration changes associated with neuronal activation in the human brain ¿H. Eda, I. Oda, Y. Ito, Y. Wada, Y. Oikawa, Y. Tsunazawa, M. Takada, Y. Tsuchiya, Y. Yamashita, M. Oda, A. Sassaroll, Y. Yamada, M. Tamura, Multi-channel time-resolved optical tomographic imaging system, Rev. Sci. Instrum., 70 (1999) 3595-3602. Here, we used this optical imaging system to demonstrate that the backward digit span (DB) task activated the dorsolateral prefrontal cortex (DLPFC) of each hemisphere more than the forward digit span (DF) task in healthy adult volunteers, and higher performance of the DB task was closely related to the activation of the right DLPFC. These results suggest that visuospatial imagery is a useful strategy for the DB task. Optical tomography described here is a new modality of neuropsychological studies.

Non-synchronous behavior of neuronal activity, oxidative metabolism and blood supply during mental tasks in man.

In near-infrared spectroscopic studies during mental tasks such as problem solving and mental arithmetic, we found that 9 of 33 healthy volunteers showed decreases in both the regional cerebral blood flow (r-CBF) and oxygen consumption rate (CMRO2) in the frontal region of the dominant hemisphere. To confirm these unexpected observations, we performed simultaneous measurements by positron emission tomography (PET) and near-infrared spectroscopy (NIRS) in two such subjects. PET images also showed that CBF decreased within the presumptive area illuminated by near-infrared light during mental task. However, CBF decreased in almost all regions while the subject gave a correct answer. Thus, the questions arose: Are mental tasks always associated with increases in r-CBF and/or CMRO2?

Near-infrared optical detection of sequential brain activation in the prefrontal cortex during mental tasks.

To examine the spatiotemporal differences of brain activation during mental tasks, changes in the oxygenation and hemodynamics in two regions of the prefrontal cortex were measured simultaneously by near-infrared spectroscopy (NIRS). Subjects were eight healthy adults who attempted to solve three different mathematical problems. The behavior of concentration changes in oxy-, deoxy-, and total hemoglobin in one brain region varied with the time course (more than 10 min). This suggested that regional brain activity varied during the performance of the mental task. In each single subject, the pattern of these changes varied with each problem, and this variation differed from subject to subject. When NIRS traces in two regions were compared, it was seen that activated regions moved alternatively: when in one region total hemoglobin that had first increased returned to the resting level, in the other it started to increase. These region-dependent temporal variations of brain activity might reflect mental processes. It is thus concluded that NIRS has the potential for imaging the sequence of brain activation.

Quantitative evaluation of the relative contribution ratio of cerebral tissue to near-infrared signals in the adult human head: a preliminary study

Combining spatially- and time-resolved spectroscopies. we attempted to quantitatively evaluate the contribution ratio of the partial mean pathlength of cerebral tissue to the observed overall mean pathlength, in which haemoglobin concentrations were selectively changed by administration of acetazolamide. When acetazolamide was administered, the observed increases in oxygenated haemoglobin depended on the probe distance, which became progressively larger at distances of 2, 3 and 4 cm. Increases in oxygen saturation were detected at 3 and 4 cm spacing, but not at 2 cm. Assuming that the modified Lambert-Beers law can exist in the inhomogeneous structure of the head, then, we could estimate the contribution ratio of the cerebral tissue to optical signals at the probe distances of 2, 3 and 4 cm as 33%, 55% and 69%, respectively. Using these values, we recalculated acetazolamide-induced concentration changes in oxygenated-haemoglobin in the cerebral tissue, which resulted in the same values at distances of 2, 3 and 4 cm as expected. Thus, our present method opened the door to the possibility of selectively obtaining optical signals attributed to cerebral tissue.

Relationship between fluctuations in the cerebral hemoglobin oxygenation state and neuronal activity under resting conditions in man.

Simultaneous measurements by near-infrared spectroscopy and electroencephalography were performed during 15-min resting periods in nine healthy adult brains. The peak frequencies of the frontal and occipital rhythms varied with the time course like the hemoglobin oxygenation state. Changes in the amount of oxygenated hemoglobin in the frontal region by more than 50% of the maximum resting variation range were accompanied by changes in peak frequency at the electrode position between two optodes. The results suggest that spontaneous neuronal activity is responsible for fluctuations in the hemoglobin oxygenation state in the resting state.

Dynamic features of hemodynamic and metabolic changes in the human brain during all-night sleep as revealed by near-infrared spectroscopy

By the use of near-infrared spectroscopy, hemodynamic and metabolic changes were monitored continuously in the human brain during all-night sleep in a similar time dimension as closely monitored by electroencephalography. Measurements were started in the awake state, the values of which were taken as the control. Contrary to what many have predicted, the cerebral oxygen metabolic rate (CMRO2) increased during the transition from wakefulness to sleep. Cerebral blood flow (CBF) decreased during non-rapid eye movement (non-REM) sleep, in which a dissociation between changes in CBF and those in CMRO2 was observed. The CBF returned to the control level even in response to the only 20-s appearance of alpha activity on the electroencephalogram. During REM sleep both CBF and CMRO2 were practically the same as the control level, whereas during the transition from REM sleep to arousal a disproportionate increase in CBF compared with CMRO2 was observed. Thus, it is suggested that the flow-metabolic coupling mechanism is reset to a new level during sleep.

Hemodynamic responses to photic stimulation in neonates.

A three-channel near-infrared monitoring system was used to evaluate the regional hemodynamic responses to photic stimulation during spontaneous sleep in seven healthy neonates. Three pairs of parallel light guides, separated by 15 mm each, were placed over a 450-mm(2) occipital region of the head. Increases in oxygenated and total hemoglobin were observed during photic stimulation only in one channel, and no change or decreases in oxygenated, deoxygenated, and total hemoglobin were observed in the other two channels. The change in the direction of deoxygenated hemoglobin accompanying the increases in oxygenated and total hemoglobin (usually a decrease in adults) differed in each subject and also varied with each measurement even in the same subject. An increase, decrease, and no change were observed. The results imply that an increase in regional cerebral blood flow occurs because of stimulation specific to the visual cortex and that the increase in deoxygenated hemoglobin observed in the visual cortex of the neonatal brain is attributable to venous dilation.

Near-infrared monitoring of cerebral oxygenation state during carotid endarterectomy

BACKGROUNDnRecent studies have indicated that near-infrared spectroscopy (NIRS) could continuously and noninvasively observe the changes in cerebral oxygenation state during hypoxia and ischemia, using their optical properties. Its validity and usefulness during cerebrovascular surgery, however, still remain to be clarified.nnnMETHODSnUsing NIRS, we continuously monitored the changes in the concentration of oxyhemoglobin, deoxyhemoglobin, and total hemoglobin ([oxy-HB], [deoxy-Hb], and [total Hb], respectively) and redox state of cytochrome oxidase (cyt ox) during carotid endarterectomy for 22 patients, and we compared the NIRS responses with those of intraoperative somatosensory evoked potentials (SEP) and regional cerebral blood flow (rCBF).nnnRESULTSnIn 9 of 22 patients, cross-clamping of the carotid artery caused a continuous decrease [oxy-Hb] and [total Hb], and an increase in [deoxy-Hb]. Cyt ox was partially reduced during the clamping. These NIRS responses demonstrated the occurrence of severe hypoxia in the ipsilateral cerebral tissue. These patients showed a marked decrease in the N20 amplitude of SEP and rCBF. In contrast, the other 13 patients did not show a significant decrease in the cerebral oxygenation state, which showed no remarkable changes in either SEP or in rCBF.nnnCONCLUSIONSnNIRS could successfully jude the cerebral oxygenation state noninvasively during carotid surgery and was more sensitive to ischemic crisis than other indirect methods.

Dynamic changes in cerebral oxygenation in chemically induced seizures in rats : study by near-infrared spectrophotometry

Using near-infrared spectrophotometry, the redox state of copper in cytochrome oxidase, and the hemoglobin oxygenation state were measured in the rat brain in situ during and after chemically induced seizures. Pentylentetrazol (PTZ) administration caused the partial reduction of cytochrome oxidase in the brain just before the electroencephalogram (EEG) showed desynchronization, and then blood pressure was elevated concomitantly with an increase in cerebral blood volume. When blood pressure reached a maximum, bursts of spikes appeared on the EEG and cytochrome oxidase was reoxidized to reach the initial oxidation level, giving a rapid, transient reduction of cytochrome oxidase in the preictal period. Hemoglobin was more oxygenated than before the administration throughout the seizure. In the late postictal phase, cytochrome oxidase was partially reduced again, while blood pressure remained high and hemoglobin was more oxygenated than initially. The second administration of PTZ in the late postictal phase induced the same responses as observed after the first administration. By decreasing oxygen concentrations in the inspired gas during the seizure, cytochrome oxidase was more reduced than in the non-epileptic rat, and spike activity was observed until about 85% of cytochrome oxidase was reduced. The transient cerebral hypoxia reflected by the reduction of cytochrome oxidase in the preictal period may be a trigger for an increase in cerebral blood flow rather than the result of a delayed autonomic response. The second reduction of cytochrome oxidase observed in the late postictal phase may be due to a lasting arterio-venous shunt that opens during seizures. These results revive the classical theory that cellular hypoxia is responsible for epileptic brain damage.