Yong-Sang Park

Association between Numerical Variations of Vertebrae and Carcass Traits in Jeju Native Black Pigs, Landrace Pigs, and Crossbred F 2 Population

The number of thoracic and lumbar vertebrae is known to be an unfixed trait among mammals. This study focused on the relationship between numerical variations of cervical (CER), thoracic (THO), and lumbar (LUM) vertebrae and the total number of vertebrae (TNV) and carcass traits in Jejunative black pigs (JBPs), Landrace pigs, and their intercrossed population. There were no numerical variations in CER vertebrae. On the other hand, the numbers of THO and LUM vertebrae and the TNV varied in all three populations. Of the traits investigated in the three populations, only the meansSE of the LUM vertebrae did not show statistical significance (p>0.05). The carcass weights (CW), meat color (MC), marbling score (MS), backfat thickness (BFT), carcass length (CLE), THO vertebrae, and TNV all showed statistical significance (p population had 14-17 THO vertebrae, 5-7 LUM vertebrae, and 27-30 TNV. In the F2 population, increased numbers of THO vertebrae and TNV were associated with a significant increase in the CW, CLE, and BFT (pThe number of thoracic and lumbar vertebrae is known to be an unfixed trait among mammals. This study focused on the relationship between numerical variations of cervical (CER), thoracic (THO), and lumbar (LUM) vertebrae and the total number of vertebrae (TNV) and carcass traits in Jejunative black pigs (JBPs), Landrace pigs, and their intercrossed F 2 population. There were no numerical variations in CER vertebrae. On the other hand, the numbers of THO and LUM vertebrae and the TNV varied in all three populations. Of the traits investigated in the three populations, only the means±SE of the LUM vertebrae did not show statistical significance (p>0.05). The carcass weights (CW), meat color (MC), marbling score (MS), backfat thickness (BFT), carcass length (CLE), THO vertebrae, and TNV all showed statistical significance (p 2 population had 14-17 THO vertebrae, 5-7 LUM vertebrae, and 27-30 TNV. In the F 2 population, increased numbers of THO vertebrae and TNV were associated with a significant increase in the CW, CLE, and BFT (p<0.05). In particular, the increase in the TNV was caused by an increase in the number of THO rather than LUM vertebrae. Although the animals with a greater number of THO and TNV had thicker backfat, they had a longer CLE and a heavier CW. Both these traits are economically more important than the level of backfat when determining the productivity level. These results suggest that genetic selection to increase the number of vertebrae, especially in Landrace pigs, JBPs, and their related populations, may be an excellent strategy for improving productivity.

Comparison of AndroMed and Triladyl Extender for Freezing of Jeju Native Black Bull Semen

Educational Science Reserch Institute, Jeju National University, Jeju 690-756, Korea This study was carried out to investigate synthetic extender for semen cryopreservation of Jeju Native Black Bull. The semen was collected using an artificial vagina and transported to the laboratory. The semen was diluted 1:1 by Tris-Egg yolk extender and contrifuged in 1,500 rpm for 15 minutes. The supernatant was removed. The pellect was diluted to final sperm concentration of 2×10

Effect of Dietary Supplementation of Dried-Citrus Pulp and Wheat Bran on Growth and Meat Quality in Horses

Kangwon National University, Chuncheon-si,Gan-Do, 200-701, KoreaABSTRACTThis study was conducted to elucidate the characteristics of equine meat traits and to evaluate the utilization of dried-citrus pulp abundantly produced in Jeju Island as feed ingredient in the diet of finished meat horses. Treatment groups consisted of wheat bran (A) and the dried-pellet mixture of citrus pulp(20%) and wheat bran(80%) (B). Feeding of agricultural by-products were 2 times in a day at the level of 1.5% of body weights. Water and dried-grass forage was not restricted. The average daily gain was similar in both groups. Feed conversion of treatment B showed lower level than that of A. Carcass rate of B was relatively lower than that of A, but meat yield grade of B was higher than that of A. For meat color traits, L* of B was lighter than that of A, and b* of group B was lower than that of group A. From the analysis of physical characteristics of equine meats, shearing force had decreased in the treatment B but the levels of drip loss and water holding cavity had increased in the treatment B compared to A. The level of crude fat is lower in the treatment B than the treatment A. For the mineral contents, the treatment B showed higher levels of Ca, K, Mg, Cu and Mn than that of A. For the fatty acid contents, the treatment B showed increased levels of stearic acid, oleic acid, eicosenoic acid, and arachidonic acid compared to A. Consequently, these results suggested that dried-citrus pulp might be utilized as replacement material for equine forage. Please rewrite the whole abstract and should include all required information. Before resubmission, please check it again from a native colleague in your group. (Key words :Dried-citrus pulp, Wheat bran, Horse, Growth, Meat quality)

Polymorphisms and Allele Distribution of Novel Indel Markers in Jeju Black Cattle, Hanwoo and Imported Cattle Breeds

The aim of this study was to screen the polymorphisms and distribution of each genotype of in-sertion/deletion (indel) markers which were found in a preliminary comparative study of bovine ge-nomic sequence databases. Comparative bioinformatic analyses were first performed between the nu-cleotide sequences of Bovine Genome Project and those of expressed sequence tag (EST) database, and a total of fifty-one species of indel markers were screened. Of these, forty-two indel markers were evaluated, and nine informative indel markers were ultimately selected for population analysis. Nucleotide sequences of each marker were re-sequenced and their polymorphic patterns were typed in six cattle breeds: Holstein, Angus, Charolais, Hereford, and two Korean native cattle breeds (Hanwoo and Jeju Black cattle). Cattle breeds tested in this study showed polymorphic patterns in eight indel markers but not in the Indel-15 marker in Charolais and Holstein. The results of analysis for Jeju Black cattle (JBC) population indicated an observed heterozygosity (Ho) that was highest in HW_G1 (0.600) and the lowest in Indel_29 (0.274). The PIC value was the highest in HW_G4 (0.373) and lowest in Indel_6 (0.305). These polymorphic indel markers will be useful in supplying genetic information for parentage tests and traceability and to develop a molecular breeding system for im-provement of animal production in cattle breeds as well as in the JBC population. Keywords : Indel marker, polymorphism, genotype, cattle, Jeju black cattle

Genetic Characterization of Wolla Coat Color in Jeju Horses

ABSTRACT This study was carried out to define the “Wolla” coat color using 376 Jeju registered horses (white patched 142, solid coat color 234). Three major factors related to the white patches i.e ECA3-inversion for Tobiano, EDNRB 2 bp nucleotide substitution for frame Overo, and the KIT intron 16 single nucleotide polymorphism (SNP) for Sabino types of coat color were analyzed. It was found that out of 142 Jeju horses with white patches that have the genotype for ECA3-inversion ( To ) 140 horses were + /To heterozygous and 2 horses were To/To homozygous all Jeju horses with white patches had ECA3-inversion allele. However, there was no frame Overo or Sabino allele type in EDNRB and KIT intron 16 SNP in Jeju horses with white patches. As for 234 Jeju horses with a solid coat color, there was no ECA3-inversion allele related to the white patches. Thus, it could be considered that Wolla coat color with white patches in Jeju horses might have come from the Tobiano line in the genetic classification by coat color.(

The Conformation of the Jindo (Canis familiaris), Korean Native Dog - the trunk and limb

Park, Y. S.*, S. K. Kim**, J. C. Lee**, S. I. Oh**, S. H. Lee*, B. K. Park*,J. I. Lee**, C. Y Lee** and C. G. Lee**Department of Companion and Laboratory Animal Science,College of Industrial Science, Kongju National University*,College of Veterinary Medicine, Chonnam National University**ABSTRACTThe trunk and limb of the Jindo, Korean native dog was measured in the present study. One hundredand ninety five Jindoes were randomly selected from the 1,985 dogs in the Jindo County in August 1998,which were screened by the judging committee of the County and registered by inserting electronic chipsin the withers. They were about a year and half old, and were white and fawn dogs. A total of 18 partsof the trunk and limb were measured using calipers and tapes. The average height at the withers was50.67 cm in dogs and 47.48 cm in bitches. The average body length was 52.99 cm in dogs and 50.33 cmin bitches. And the average height : body length ratio was 104.70 in dogs and 106.08 in bitches. Nodifferences were observed between the Jindoes of the two coat colors in these measurements. The resultsof this study would be useful for compiling the official standard for the adult Jindo.(Keywords : Jindo, Korean native dog, Trunk and limb measurements, 18 parts)

PCR-based Detection of Bovine Papillomavirus DNA from the Cutaneous Papillomas and Surrounding Environments in the Korean Native Cattle, Hanwoo

Two 1-year old calves of Korean Native cattle (Hanwoo) presented cutaneous papillomas on the face and neck. Type 2 bovine papillomavirus (BPV-2) was identified in the cutaneous papillomas based on BPV-specific PCR and subsequent DNA sequencing analysis results. Using DNA samples extracted from two affected calves and unaffected animals reared in the same stable, BPV-2 was not only detected in the cutaneous papillomas of affected animals based on BPV-specific PCR analysis, but also detected in normal skins, hairs, and their environments based on nested PCR analysis. BPV-2 was also detected in DNA samples isolated from animals and environments of that distinct stable with affected calves. However, no BPV-2 was detected in the drinking water of both stables (infected and unaffected). These findings concluded that BPV-2 was transmitted by direct or indirect contact, not by drinking water. This is the first report to show molecular evidence of BPV-2 infection. Rapid and precise molecular identification can be used to screen BPV-2 in cattle farms to understand the biological roles of BPV in animal diseases.

Embryo Gender Ratio and Developmental Potential after Biopsy of In Vivo and In Vitro Produced Hanwoo Embryos

The present study was to assess the in vitro viability and sexing rate of bovine embryos. Blastocysts were harvested on day 7~9 day after insemination(in vitro and in vivo), and the sex of the embryos was examined using the LAMP method. Embryo cell biopsy was carried out in a 80 μl drop Ca, Mg 2+ free D-PBS and, biopsied embryos viability were evaluated after more 12 h culture in IVMD culture medium. The formation of recovered embryo to expanded and hatching stages had ensued in higher of sexed embryo in vivo than in vitro (100% vs. 89%, p<0.05), and in vitro, the rates of degeneration after sexing were significantly (p<0.05) higher in vitro than in vivo(11% vs. 0.0%). The rates of the predicted sex were female 61% vs. 56%, and male 39% vs. 44% in vivo and in vitro, respectively. The rates of survival following different biopsy methods were seen between punching and bisection method in vivo and in vitro (100% vs. 89% and 100% vs, 78% respectively). Biopsy method by punching was significantly (p<0.05) higher than bisection between produced embryos in vivo and in vitro. The present data indicate that with microblade after punching for embryo sexing results in high incidence of survivability on development after embryo biopsy. It is also suggested that LAMP-based embryo sexing suitable for field applications. (