Yongchang Wang

Functional degradation of visual cortical cells in old cats.

Visual function declines with age. Using extracellular single-unit in vivo recordings, we compared the function of primary visual cortical (area 17) cells in young and old paralyzed, anesthetized cats. The results reveal that cortical neurons in old cats exhibit higher visually evoked responses, higher spontaneous activities, lower signal-to-noise ratios, and weaker orientation and direction selectivity than do cells in young adult cats. These findings are consistent with previously reported age related declines in cortical function in senescent macaque monkeys. Thus, similar declines in cortical function accompany old age in different mammalian species with well developed cortices.

Neural correlates of boundary perception

The responses of neurons in areas V1 (17) and V2 (18) of anesthetized and paralyzed rhesus monkeys and cats were recorded while presenting a set of computer-generated visual stimuli that varied in pattern, texture, luminance, and contrast. We find that a class of extrastriate cortical cells in cats and monkeys can signal the presence of boundaries regardless of the cue or cues that define the boundaries. These cue-invariant (CI) cells were rare in area V1 but easily found in V2. CI cortical cells responded more strongly to more salient boundaries regardless of the cue defining the boundaries. Many CI cortical cells responded to illusory contours and exhibited the same degree of orientation and direction selectivity when tested with boundaries defined by different cues. These cells have significant computational power inherent in their receptive fields since they were able to generalize across stimuli and integrate multiple cues simultaneously in order to signal boundaries. Cells in higher order cortical areas such as MT (Albright, 1992), MST (Geesaman & Anderson, 1996), and IT (Sary et al., 1993) have previously been reported to respond in a cue invariant fashion. The present results suggest that the ability to respond to boundaries in a cue-invariant manner originates at relatively early stages of cortical processing.

Functional degradation of extrastriate visual cortex in senescent rhesus monkeys

The receptive field properties of striate cortical (V1) cells degrade in senescent macaque monkeys. We have now carried out extracellular single unit studies of the receptive field properties of cells in extrastriate visual cortex (area V2) in very old rhesus (Macaca mulatta) monkeys. This study provides evidence that both the orientation and direction selectivities of V2 cells in old monkeys degrade significantly. Decreased selectivity is accompanied by increased visually driven and spontaneous responses. As a result, V2 cells in old animals exhibit markedly decreased signal-to-noise ratios. A significant degradation of neural function in extrastriate cortex may underlie the declines in higher order visual function that accompany normal aging.

Aging affects the direction selectivity of MT cells in rhesus monkeys

The ability to accurately perceive the direction and speed of moving objects declines during normal aging. This is likely due to functional degradation of cortical neurons. Most neurons in the primate middle temporal area (MT) are direction-selective and their activity is closely linked to the perception of coherent motion. We investigated the mechanisms that underlie this age-related decline by comparing the proportions of direction-selective MT cells in old and young macaque monkeys, using in vivo single-cell recording techniques. Our results showed that the proportion of such cells was lower in old than in young monkeys. Moreover, one type of direction-sensitive cells, pattern cells, was especially sensitive to aging and was affected more severely than another class, component cells. We also found that direction selectivity was affected more severely in MT than in V1 of senescent monkeys. Thus, the functional degradation of MT and V1 cells may mediate perceptual decline in visual motion tasks in old primates.

Aging affects contrast response functions and adaptation of middle temporal visual area neurons in rhesus monkeys

In the present study we studied the effects of aging on the coding of contrast in area V1 (primary visual cortex) and MT (middle temporal visual area) of the macaque monkey using single-neuron in vivo electrophysiology. Our results show that both MT and V1 neurons in old monkeys are less sensitive to contrast than those in young monkeys. Generally, contrast sensitivity is affected by aging more severely in MT cells than in V1 cells. Specifically, MT cells were affected more severely than motion direction selective V1 cells. Particularly, we found that MT neurons in old monkeys exhibited enhanced maximum visual responses, higher levels of spontaneous activity and decreased signal-to-noise ratios. In addition, we also found age-related changes in neuronal adaptation to visual motion in MT. Compared with young animals, the contrast gain of MT neurons in old monkeys is less affected, but the response gain by adaptation of MT neurons is more affected. Our results suggest that there may be an anomalous visual processing in both the magnocellular and parvocellular pathways. The neural changes described here are consistent with an age-related degeneration of intracortical inhibition and could underlie some deficits in visual function during normal aging.

Aging Affects the Neural Representation of Speed in Macaque Area MT

Human perception of speed declines with age. Much of the decline is probably mediated by changes in the middle temporal (MT) area, an extrastriate area whose neural activity is linked to the perception of speed. In the present study, we used random-dot patterns to study the effects of aging on speed-tuning curves in cortical area MT of macaque visual cortex. Our results provide evidence for a significant degradation of speed selectivity in MT. Cells in old animals preferred lower speeds than did those in young animals. Response modulation and discriminative capacity for speed in old monkeys were also significantly weaker than those in young ones. Concurrently, MT cells in old monkeys showed increased baseline responses, peak responses and response variability, and these changes were accompanied by decreased signal-to-noise ratios. We also found that speed discrimination thresholds in old animals were higher than in young ones. The foregoing neural changes may mediate the declines in visual motion perception that occur during senescence.

Spatial and temporal sensitivity degradation of primary visual cortical cells in senescent rhesus monkeys

Human visual function declines with age. Much of this decline is mediated by changes in the central visual pathways. In this study we compared the spatial and temporal sensitivities of striate cortical cells in young and old paralysed macaque monkeys. Extracellular single‐unit recordings were employed. Our results show that cortical neurons in old monkeys exhibit lower optimal spatial and temporal frequencies, lower spatial resolution and lower high temporal frequency cut‐offs than do cells in young adult monkeys. These changes in old monkeys are accompanied by increased visually evoked responses, increased spontaneous activities and decreased signal‐to‐noise ratios. The increased excitability of cells in old animals is consistent with an age‐related degeneration of intracortical inhibition. The degradation of spatial and temporal function in old striate cortex should contribute to the decline in visual function that accompanies normal aging.

Aging affects response variability of V1 and MT neurons in rhesus monkeys.

Visual function declines with age. Much of the decline may result from functional degradation in central visual areas. To investigate the physiological mechanisms underlying visual function declines during normal aging, we compared the response variability of cells in primary visual cortex (V1) and middle temporal visual area (MT) in young adult and very old macaque monkeys using single-neuron in vivo electrophysiology. We found that mean response and response variability in both V1 and MT of old monkeys are significantly higher than in young monkeys. And response-to-noise ratio in old monkeys is significantly lower than in young ones. The results are consistent with an age-related degradation of inhibitory intracortical circuits. The neural changes described here could contribute to declines in visual function during senescence.

Functional Degradation of the Primary Visual Cortex During Early Senescence in Rhesus Monkeys

Visual function in humans degrades during the early stage of senescence beginning from middle 50s to 60s. To identify its underlying neural mechanisms, we investigated the aging effects on the primary visual cortex (V1) cells in early senescent (ES) monkeys (Macaca mulatta). Under anesthesia, receptive field properties of V1 cells were examined by extracellular single-unit recordings in the young adult (YA; 5-6 years old), ES (19-24 years old), and late senescent (LS; 28-32 years old) monkeys. We found clear indications of functional degradation in early senescence, including impaired stimulus selectivities, increased level of spontaneous activity and declined signal-to-noise ratio, and dynamic range of V1 cell responses. Importantly, the functional degradation in early senescence exhibited unique features that were different from the results for the LS animals, such as remarkable individual variability in orientation selectivity and unchanged peak response elicited by visual stimulation. Our results demonstrate that the function of V1 degrades during the early stage of aging in nonhuman primate, suggesting potential neural correlates for functional deficits observed in early senescence in human subjects. Moreover, these results provide new insight into the dynamics of the aging-related functional deterioration, revealing a more complex and heterogeneous picture of this process.

The effects of aging on the strength of surround suppression of receptive field of V1 cells in monkeys.

The surround suppression of the receptive field is important for basic visual information processing, such as orientation specificity. To date, the effects of aging on the strength of surround suppression are not clear. To address this issue, we carried out extracellular single-unit studies of the receptive field properties of cells in the primary visual cortex (area V1) in young and old rhesus (Macaca mulatta) monkeys. When presented with the oriented central stimulus, we found that cells in old animals showed reduced orientation and direction selectivity compared with those in young animals. When presented with the oriented central stimulus together with the optimal surround stimulus, more selective cells {orientation bias (OB) >/=0.1; a bias of 0.1 is significant at the P<0.005 level} in animals of both ages showed reduced orientation selectivity compared with the experiment that presented only the oriented central stimulus. When presented with the optimal central stimulus together with the oriented surround stimulus, cells in old animals showed reduced orientation and direction selectivity compared with young animals. Moreover, broadly tuned cells (OB<0.1) in old animals exhibited significantly reduced suppression indices that quantified the strength of the surround suppression of the receptive field, when compared with those in young animals. These results suggest that aging may seriously affect the surround suppression of the receptive field of V1 cells. Thus, the decreased strength of surround suppression of the receptive field may be one possible reason for the decreased stimulus selectivity of V1 cells previously found in the senescent brain. This work will contribute to an understanding of the physiological mechanisms mediating surround suppression of the receptive field.