Yoshiaki Nakai
Hiroshima University
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Publication
Featured researches published by Yoshiaki Nakai.
PLOS ONE | 2013
Kyoko Ueda; Ryoko Kawabata; Takashi Irie; Yoshiaki Nakai; Yukinobu Tohya; Takemasa Sakaguchi
Tannins, plant-derived polyphenols and other related compounds, have been utilized for a long time in many fields such as the food industry and manufacturing. In this study, we investigated the anti-viral effects of tannins on 12 different viruses including both enveloped viruses (influenza virus H3N2, H5N3, herpes simplex virus-1, vesicular stomatitis virus, Sendai virus and Newcastle disease virus) and non-enveloped viruses (poliovirus, coxsachievirus, adenovirus, rotavirus, feline calicivirus and mouse norovirus). We found that extracts from persimmon (Diospyros kaki), which contains ca. 22% of persimmon tannin, reduced viral infectivity in more than 4-log scale against all of the viruses tested, showing strong anti-viral effects against a broad range of viruses. Other tannins derived from green tea, acacia and gallnuts were effective for some of the viruses, while the coffee extracts were not effective for any of the virus. We then investigated the mechanism of the anti-viral effects of persimmon extracts by using mainly influenza virus. Persimmon extracts were effective within 30 seconds at a concentration of 0.25% and inhibited attachment of the virus to cells. Pretreatment of cells with the persimmon extracts before virus infection or post-treatment after virus infection did not inhibit virus replication. Protein aggregation seems to be a fundamental mechanism underlying the anti-viral effect of persimmon tannin, since viral proteins formed aggregates when purified virions were treated with the persimmon extracts and since the anti-viral effect was competitively inhibited by a non-specific protein, bovine serum albumin. Considering that persimmon tannin is a food supplement, it has a potential to be utilized as a safe and highly effective anti-viral reagent against pathogenic viruses.
Journal of Food Science | 2014
Maki Kamimoto; Yoshiaki Nakai; Toru Tsuji; Toshi Shimamoto; Tadashi Shimamoto
UNLABELLED Human noroviruses (NoVs) are the leading cause of gastroenteritis and foodborne illnesses worldwide. In this study, we investigated the effects of persimmon extract (PE) on NoV GII.4 and bacteriophage MS2. We also examined the relationship between the tannin content of PE and its antiviral effects to identify the active ingredient in PE. Different persimmon tannin (PT) solutions were prepared by mixing PE with different concentrations of bovine serum albumin. The antiviral efficacy of these solutions against NoV was evaluated by quantifying the amount of residual noroviral genome using a quantitative reverse transcription PCR (qRT-PCR) assay. The antiviral efficacy of PE against MS2 was examined with an infectivity assay (plaque assay). Solutions containing ≥ 0.11 mg/mL PT reduced the noroviral genome by more than 70.0% and the infectivity of MS2 by more than 2.5 log PFU/mL. However, the effects of PT on both viruses decreased markedly at a concentration of 0.08 mg/mL and solutions containing negligible PT had no antiviral activity. These results suggest that the PT component of PE inactivates NoV and MS2. Our results indicate that PE is a nontoxic antiviral agent effective against enteric viruses. PRACTICAL APPLICATION Persimmon extract showed antiviral effects against NoV and bacteriophage MS2. Persimmon extract is suitable for use as an antiviral agent.
Archive | 2008
Tadashi Shimamoto; Toru Tsuji; Yoshiaki Nakai
Archive | 2009
Tadashi Shimamoto; Yasushi Okinaka; Takemasa Sakaguchi; Toru Tsuji; Yoshiaki Nakai
Archive | 2005
Yoshiaki Nakai; Toru Tsuji; 義昭 中井
Archive | 2009
Tadashi Shimamoto; 整 島本; Yasushi Okinaka; 泰 冲中; Takemasa Sakaguchi; 剛正 坂口; Toru Tsuji; Yoshiaki Nakai; 中井 義昭
Archive | 2012
Tadashi Shimamoto; Toru Tsuji; Yoshiaki Nakai
Archive | 2011
Tadashi Shimamoto; 整 島本; Maki Kamimoto; 真紀 神本; Yoshiaki Nakai; 義昭 中井; Toru Tsuji
Archive | 2011
Tadashi Shimamoto; 整 島本; Yoshiaki Nakai; 義昭 中井; Toru Tsuji
Archive | 2013
Tadashi Shimamoto; Yasushi Okinaka; Takemasa Sakaguchi; Toru Tsuji; Yoshiaki Nakai