Yoshikazu Hirota
Agricultural Research Organization, Volcani Center
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Publication
Featured researches published by Yoshikazu Hirota.
Journal of Immunological Methods | 1980
Yoshikazu Hirota; Olli Vainio; Takashi Sawada; Yukio Bito
A simple plaque method for detecting cells producing antibodies of specific immunoglobulin class in the chicken is described. This method is based upon the inability of chicken antibody to activate guinea-pig complement. IgM- and IgG-specific plaque-forming cells in the spleen of chickens immunized with sheep red blood cells were detected using guinea-pig complement and rabbit anti-chicken-mu- or gamma-chain serum. The specificity of the immunoglobulin class of plaques was confirmed by the abolition of class-specific hemolytic plaques after treatment of the lymphoid cells with rabbit antisera specific for chicken heavy chains in the presence of guinea-pig complement. The method is very simple and rapid, and IgM and IgG specific plaques are reliably detected.
Journal of Veterinary Medical Science | 2002
Moshira El-Abasy; Maki Motobu; Kameo Shimura; Ki-Jeong Na; Chung-Boo Kang; Kenji Koge; Takashi Onodera; Yoshikazu Hirota
Journal of Veterinary Medical Science | 2003
Moshira El-Abasy; Maki Motobu; Ki-Jeong Na; Kameo Shimura; Kikuyasu Nakamura; Kenji Koge; Takashi Onodera; Yoshikazu Hirota
International Immunopharmacology | 2004
Moshira El-Abasy; Maki Motobu; Kikuyasu Nakamura; Kenji Koge; Takashi Onodera; Olli Vainio; Paavo Toivanen; Yoshikazu Hirota
Journal of Veterinary Medical Science | 2003
Moshira El-Abasy; Maki Motobu; Toshiya Sameshima; Kenji Koge; Takashi Onodera; Yoshikazu Hirota
International Immunopharmacology | 2004
Said A. Amer; Ki-Jeong Na; Moshira El-Abasy; Maki Motobu; Yukari Koyama; Kenji Koge; Yoshikazu Hirota
Archive | 2001
Kenji Furuya; Yoshikazu Hirota; Takeo Mizutani; Kikuyasu Nakamura; Kameo Shimura; 菊保 中村; 健二 古家; 良和 廣田; 亀夫 志村; 武雄 水谷
Archive | 2003
Tadashi Ehashi; Kenji Furuya; Yoshikazu Hirota; 健二 古家; 好和 廣田; 正 江橋
International Immunopharmacology | 2004
Moshira El-Abasy; Maki Motobu; Kikuyasu Nakamura; Kenji Koge; Takashi Onodera; Olli Vainio; Paavo Toivanen; Yoshikazu Hirota
Bulletin of the University of Osaka Prefecture. Ser. B, Agriculture and biology | 1980
Yoshikazu Hirota; Yukio Bito
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