Yoshiro Masada

Studies on monoterpene glucosides and related natural products. XXXI. Gas chromatography and gas chromatography-mass spectrometry of iridoid and secoiridoid glucosides.

A total of 33 iridoid and secoiridoid glucosides were detected by gas chromatography on several columns such as OV-1 or OV-17. Representative glucosides were then subjected to gas chromatography-mass spectrometry, giving some characteristic peaks that permitted the discrimination of both types of glucosides from other compounds in most instances. The successful detection of both types of glucosides in several plant extracts showed the applicability of this combination of methods to small amounts of plant materials.

Study on Sterols of Starfishes by GC-MS

Sterols in Starfishes(Asterina pectinifera)include at least ten closely related compounds. The difference in mass spectra between Δ5 and Δ7-sterols has been discussed. The results suggest that they had similar sterol components of characteristicΔ7-sterols. Δ7-eholestenol, Δ7, 22-ergostadienol, stellastenol, Δ7, 22-stigmastadienol, Δ7-stigmastenol, chole stanol, andΔ7.22-cholestadienol were separated by gas chromatography with 3% NGS-XE 60 column and identified by the combined gas chromatographymass spectrometry(GC-MS).

Analysis of 11-deoxy-17-ketosteroids

通常17-ヶトステロイド (以下17KSと略記) は, トリメチルシリル (TMSi) 誘導体としたのち, ガスクロマトグラフィー (GLC) による分析を行なっている.この方法には, 誘導体生成反応に際しての損失およびその誘導体が不安定で保存がきかないなどの難点がある.著者らは, TMSi誘導体としない形の17KSのGLCについての検討を行ない, TMSi誘導体としたときと同じような良好な分離条件を確立した.最適条件は, 3%OV-17, ガラス製カラム0.4×150cm, カラム温度230℃で水素炎イオン化検出器を使用することにより得られた.