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Featured researches published by Yoshitaka Ino.


International Archives of Allergy and Immunology | 1989

Characterization of the proteases in the crude mite extract.

Yoshitaka Ino; Thoru Ando; Michiko Haida; Kazunori Nakamura; Masahiro Iwaki; Hirokazu Okudaira; Terumasa Miyamoto

Characterization of the proteases was performed in the crude mite extract fractionated by Sephacryl S-200 gel filtration. Three peaks of protease activities were detected in the fractions. From the results of substrate specificity and susceptibility to the inhibitors, PK.1 protease (about 60 kD) is suggested to be a trypsin-like protease of mites. From the results of susceptibility to various agents, PK.2 (about 30 kD) and PK.3 (about 20 kD) proteases may be cysteine proteases, e.g., papain and cathepsin B. PK.3 protease existed in the precipitate of 60% ammonium sulfate fractionation. The data in the present study suggest the possibility that Dermatophagoides farinae I allergen might be a cysteine protease probably derived from the gastrointestinal tract of the house dust mite.


International Archives of Allergy and Immunology | 1991

Isolation of Cysteine Protease in the Crude Mite Extract, Dermatophagoides farinae

Tohru Ando; Yoshitaka Ino; Michiko Haida; Reiko Honma; Hidenori Maeda; Hiroshi Yamakawa; Masahiro Iwaki; Hirokazu Okudaira

In order to study the relationship between cysteine protease and Der f I, which is one of the major allergens in the mite, Dermatophagoides farinae, isolation of cysteine protease was attempted using various column chromatographies. Both the potent cysteine protease activity and the allergenic activity were detected in the same fractions by anion exchange chromatography on a DEAE-Sephacel, gel chromatographies and chelating Sepharose 6B chromatography. In the double immunodiffusion test, the finally isolated fraction and rabbit anti-Der f I sera reacted to give a single precipitation line which fused completely with the precipitation line formed by Der f I and anti-Der f I sera. Sequence analysis for the first 10 N-terminal amino acids from cysteine protease and Der f I were identical. These results strongly suggest that cysteine protease of mites may be Der f I allergen and that measuring cysteine protease activity may possibly become a beneficial method for detecting Der f I allergens.


International Archives of Allergy and Immunology | 2000

Involvement of Eosinophils in the Early-Phase Allergic Reaction in a Guinea Pig Rhinitis Model

Norio Imai; Atsushi Miyahara; Yuji Yamazaki; Reiko Homma; Yoshitaka Ino; Masateru Kurumi

Background: Eosinophils are found in the nasal lavage fluid (NLF) and nasal biopsies of patients with allergic rhinitis after a nasal antigen challenge, and associated not only with a late-phase allergic reaction (LPR) but also an early phase allergic reaction (EPR). Numerous studies have been carried out to clarify the participation of eosinophils in LPR or airway hyperresponsiveness. However, there has been no published report describing in detail the role of eosinophils during EPR. To better understand the involvement of eosinophils in EPR, we studied the effects of repeated antigen challenges on nasal airway responsiveness and eosinophilic inflammation in EPR using a guinea pig rhinitis model. Methods: Nasal airway responsiveness was measured as the nasal airway resistance (NAR) after nasal antigen provocation. Eosinophilic inflammation during EPR was assessed by nasal lavage and histopathological examination using two groups of animals: those in group 1 were subjected to a sensitization pretreatment only, and those in group 2 were subjected to a pretreatment of sensitization followed by repeated nasal challenges. Results: Repeated antigen challenges induced nasal hyperresponsiveness as indicated by a decrease in the antigen provocation dose and a significant increase in NAR. Furthermore, significant increases in eosinophil counts, eosinophil peroxidase (EPO) activity and protein content in NLF during EPR were observed following antigen provocation in group 2. There were significant correlations between the levels of these parameters, and albumin was the most prevalent of the proteins in NLF. Histopathological examination showed that the degree of eosinophil infiltration into the lamina propria of the nasal mucosa of the animals in group 2 was significantly and apparently higher than in group 1. Particularly, epithelial disruption and mucosal edema were significantly elevated after antigen provocation in group 2. Conclusions: These results suggest that chronic eosinophil accumulation is induced by repeated antigen challenges in the nasal tissue, and that once antigen provocation occurs, eosinophils in the tissue are activated and responsible for the amplification of EPR such as vascular permeability and mucosal edema.


Yakugaku Zasshi-journal of The Pharmaceutical Society of Japan | 1995

[Inhibitory effects of sepimostat mesilate (FUT-187) on the activities of trypsin-like serine proteases in vitro].

Kazunori Nakamura; Ayako Johmura; Minoru Oda; Yoshitaka Ino; Hiroyuki Uchiyama; Hisaya Ohtani; Hiroyuki Miyazaki; Masateru Kurumi; Yushiro Akizawa; Toshinori Oka

Inhibitory activities of FUT-187 on trypsin-like serine proteases were compared using camostat mesilate (camostat), and 4-(4-guanidino benzoyloxy)-phenyl acetic acid methanesulfonate (GBPA) known as an active metabolite of camostat in the blood. Ki values of FUT-187 on the competitive inhibition mechanism were 0.097 microM for trypsin, 0.029 microM for pancreatic kallikrein, 0.61 microM for plasma kallikrein, 0.57 microM for plasmin, 2.5 microM for thrombin, 20.4 microM for factor Xa and 6.4 microM for C1r. However, FUT-187 acted as a noncompetitive inhibitor for factor XIIa and an uncompetitive inhibitor for C1s, and Ki values for these proteases were 0.021 and 0.18 microM, respectively. Ki values of camostat for these proteases were in the range of 0.037 to 96.4 microM, and those of GBPA for the above proteases except trypsin and plasma kallikrein were higher than those of FUT-187. The inhibitory activity of FUT-187 on trypsin was not reduced by the addition of the serum at 10%, whereas, that of GBPA was reduced (4.3 fold) in terms of IC50 values. The concentration of FUT-187 required to double APTT (activated partial thromboplastin time) was 1.09 microM, while GBPA, by concentrations up to 1 mM failed to double APTT. The kinin formation by glandular kallikrein in the rat plasma was inhibited by FUT-187 with IC50 value of 0.024 microM, while camostat revealed no inhibition by concentrations up to 1 microM. The complement-mediated hemolyses in the classical and alternative pathways were also inhibited by FUT-187 with IC50 values of 0.17 and 3.5 microM, respectively, the corresponding values for camostat being 350 and 150 microM, respectively. It is concluded that FUT-187 is a potent and selective inhibitor of trypsin-like serine proteases, and its inhibitory activities are stronger than those of camostat on glandular kallikrein, factor XIIa and C1s in complement pathway.


International Archives of Allergy and Immunology | 1989

Experimental Asthma in Guinea Pigs Sensitized with Mites (Dermatophagoides farinae)

Akira Ishii; Koji Ito; Yoshitaka Ino; Terumasa Miyamoto

Guinea pigs were intraperitoneally immunized with 1, 10, 100 or 1,000 micrograms protein of an extract from mites (Dermatophagoides farinae) incorporated with aluminium hydroxide gel 3 times every 28 days. Ten days after the last injection, a bronchial provocation test was performed by the use of the mite extract. The group immunized with 1 microgram protein produced very low levels of IgE-, IgG1- and IgG2 antibodies, and did not show asthmatic response upon the challenge. Although the group immunized with 10 micrograms protein showed higher rates of asthmatic onset than other groups, the sera of this group contained lower levels of IgE antibodies than the group immunized with 100 or 1,000 micrograms protein. However, the sera of the group immunized with 10 micrograms protein showed higher ratios of IgE/IgG1, IgE/IgG2 and lower ratios of IgG1/IgG2 antibodies than the sera of the group immunized with 100 or 1,000 micrograms protein.


International Archives of Allergy and Immunology | 1991

Inhibition of Allergen-Induced Bronchoconstriction in Sensitized Guinea Pigs by Orally Administered Allergen

Akira Ishii; Yoshitaka Ino; Michiko Haida; Makoto Dohi; Matsunobu Suko; Yutaka Morita; Koji Ito; Hirokazu Okudaira

Crude mite extract (CME) was orally administered to guinea pigs sensitized to CME. It was shown that such treatment reduces the bronchoconstrictive response upon allergen provocation. Isolated tracheae taken from guinea pigs orally administered CME allergen showed less contraction in response to CME as compared to those obtained from sensitized but not orally treated animals. The oral administration of allergens seemed to attenuate the bronchial hyperresponsiveness of sensitized animals to a non-specific chemical stimulus (histamine). IgE antibodies titrated by 8 days passive cutaneous anaphylaxis, and IgG1 and IgG2 antibodies measured by ELISA were comparable in the sera obtained from animals before and after CME treatment.


Ensho | 1988

The role of plasma proteases in inflammation. The study by using FUT-175, a selective serine-protease inhibitor.

Masahiro Iwaki; Minoru Oda; Yoshitaka Ino; Yoshiko Koshiyama; Kazunori Nakamura; Masaoki Shibuya; Setsuro Fujii

Out of numerous factors relating to the pathogenesis and development of inflammation, the roles of plasma serine-proteases including, kallikrein, Clr, Cls, trypsin and plasmin are not thoroughly elucidated. To investigate the role of an enzyme, it is effective to use the selective inhibitor on it.In the present studies, authors used two pharmacological tools, i.e., FUT-175 (nafamostat mesilate), a selective inhibitor on serine-proteases and indomethacin, a selective inhibitor on cyclooxygenase and investigated the participation of serine-proteases in inflammation.At first, the pharmacological profile of FUT-175 was investigated in comparison with indomethacin. It was made clear that FUT-175 had selective inhibitory activities only on serine-proteases and no indomethacin-like activities.In the second, FUT-175 had significant suppressive effects on the representative models of inflammation including carrageenin-induced pleurisy and foot edema and adjuvant-arthritis in rats.These results suggest that plasma serine-proteases, especially complement system and kallikrein-kinin system play an important part in the processes of either acute or chronic inflammatory reactions.


Japanese Journal of Pharmacology | 1990

Pharmacological studies on 6-amidino-2-naphthyl(4-(4,5-dihydro-1H-imidazol-2-yl)amino) benzoate dimethane sulfonate (FUT-187). I: Inhibitory activities on various kinds of enzymes in vitro and anticomplement activity in vivo.

Minoru Oda; Yoshitaka Ino; Kazunori Nakamura; Shigeru Kuramoto; Kazunori Shimamura; Masahiro Iwaki; Setsuro Fujii


Folia Pharmacologica Japonica | 1989

[Inhibitory effect of N-alpha-[(S)-1-carboxy-3-phenylpropyl]-L-lysyl-L-proline (MK-0521) on angiotensin converting enzyme in vitro].

Yoshitaka Ino; Minoru Oda; Takuo Sato; Masahiro Iwaki


Folia Pharmacologica Japonica | 1989

[Antihypertensive effect of repeated oral administration of MK-0521 in 2-kidney Goldblatt hypertensive dogs].

Minoru Oda; Kunihiko Suzuki; Yoshitaka Ino; Takuo Sato; Masahiro Iwaki

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Hideaki Tsuji

Okayama Prefectural University

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Junji Terao

University of Tokushima

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Miki Hiemori

University of Tokushima

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Noriko Bando

University of Tokushima

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