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Dive into the research topics where Yoshitake Maeda is active.

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Featured researches published by Yoshitake Maeda.


Journal of Biological Chemistry | 2009

Structural Basis of the Interaction between Chemokine Stromal Cell-derived Factor-1/CXCL12 and Its G-protein-coupled Receptor CXCR4

Yutaka Kofuku; Chie Yoshiura; Takumi Ueda; Hiroaki Terasawa; T. Hirai; Sae Tominaga; Masako Hirose; Yoshitake Maeda; Hideo Takahashi; Yuya Terashima; Kouji Matsushima; Ichio Shimada

The chemokine stromal cell-derived factor-1 (SDF-1/CXCL12) and its G-protein-coupled receptor (GPCR) CXCR4 play fundamental roles in many physiological processes, and CXCR4 is a drug target for various diseases such as cancer metastasis and human immunodeficiency virus, type 1, infection. However, almost no structural information about the SDF-1-CXCR4 interaction is available, mainly because of the difficulties in expression, purification, and crystallization of CXCR4. In this study, an extensive investigation of the preparation of CXCR4 and optimization of the experimental conditions enables NMR analyses of the interaction between the full-length CXCR4 and SDF-1. We demonstrated that the binding of an extended surface on the SDF-1 β-sheet, 50-s loop, and N-loop to the CXCR4 extracellular region and that of the SDF-1 N terminus to the CXCR4 transmembrane region, which is critical for G-protein signaling, take place independently by methyl-utilizing transferred cross-saturation experiments along with the usage of the CXCR4-selective antagonist AMD3100. Furthermore, based upon the data, we conclude that the highly dynamic SDF-1 N terminus in the 1st step bound state plays a crucial role in efficiently searching the deeply buried binding pocket in the CXCR4 transmembrane region by the “fly-casting” mechanism. This is the first structural analyses of the interaction between a full-length GPCR and its chemokine, and our methodology would be applicable to other GPCR-ligand systems, for which the structural studies are still challenging.


Proceedings of the National Academy of Sciences of the United States of America | 2004

Structure of the receptor-binding domain of human thrombopoietin determined by complexation with a neutralizing antibody fragment

Michael D. Feese; Taro Tamada; Yoichi Kato; Yoshitake Maeda; Masako Hirose; Yasuko Matsukura; Hideki Shigematsu; Takanori Muto; Atsushi Matsumoto; Hiroshi Watarai; Kinya Ogami; Tomoyuki Tahara; Takashi Kato; Hiroshi Miyazaki; Ryota Kuroki

The cytokine thrombopoietin (TPO), the ligand for the hematopoietic receptor c-Mpl, acts as a primary regulator of megakaryocytopoiesis and platelet production. We have determined the crystal structure of the receptor-binding domain of human TPO (hTPO163) to a 2.5-Å resolution by complexation with a neutralizing Fab fragment. The backbone structure of hTPO163 has an antiparallel four-helix bundle fold. The neutralizing Fab mainly recognizes the C–D crossover loop containing the species invariant residue Q111. Titration calorimetric experiments show that hTPO163 interacts with soluble c-Mpl containing the extracellular cytokine receptor homology domains with 1:2 stoichiometry with the binding constants of 3.3 × 109 M–1 and 1.1 × 106 M–1. The presence of the neutralizing Fab did not inhibit binding of hTPO163 to soluble c-Mpl fragments, but the lower-affinity binding disappeared. Together with prior genetic data, these define the structure–function relationships in TPO and the activation scheme of c-Mpl.


Biotechnology Journal | 2010

Polyethylenimine‐cationized β‐catenin protein transduction activates the Wnt canonical signaling pathway more effectively than cationic lipid‐based transduction

Midori Kitazoe; Junichiro Futami; Mitsuo Nishikawa; Hidenori Yamada; Yoshitake Maeda

The Wnt canonical signaling pathway is essential for the early development of eukaryotic organisms and plays a key role in cell proliferation, differentiation, and oncogenesis. Moreover, the Wnt canonical signaling pathway contributes to the self‐renewal of mouse hematopoietic stem cells (HSCs). Here, we demonstrate artificial activation of the Wnt canonical signaling pathway by β‐catenin protein transduction. Constitutively active β‐catenin protein was introduced into human embryonic kidney HEK‐293 cells using a polyethylenimine (PEI) cationization method, or with the BioPORTER protein transduction reagent. We have previously shown that modification with PEI effectively causes proteins to be internalized by living mammalian cells. PEI‐cationized, constitutively active β‐catenin protein was added to HEK‐293 cells, and induction of several Wnt/β‐catenin target genes was detected by real‐time PCR. However, using BioPORTER to introduce active β‐catenin did not activate the Wnt canonical signaling pathway. Introduction of eGFPNuc (enhanced green fluorescent protein variant containing a nuclear localization signal) into HEK‐293 cells using the BioPORTER reagent caused significant cell death, as determined by propidium iodide staining. In contrast, the PEI‐modified eGFPNuc did not impair survival of HEK‐293 cells. These results indicate that the Wnt canonical signaling pathway could be successfully activated by transduction of PEI‐cationized active β‐catenin, and the PEI‐cationization method is an effective and safe technology for protein transduction into mammalian cells.


Protein Science | 2011

Affinity transfer to a human protein by CDR3 grafting of camelid VHH

Hidetoshi Inoue; Akiko IIhara; Hideo Takahashi; Ichio Shimada; Isao Ishida; Yoshitake Maeda

VHH is the binding domain of the IgG heavy chain. Some VHHs have an extremely long CDR3 that contributes to antigen binding. We studied the antigen binding ability of CDR3 by grafting a CDR3 from an antigen‐binding VHH onto a nonbinding VHH. cAb‐CA05‐(1RI8), the CDR3‐grafted VHH, had an antigen‐binding ability. To find a human scaffold protein acceptable for VHH CDR3 grafting, we focused on the conserved structure of VHH, especially the N‐terminal and C‐terminal amino acid residues of the CDR3 loop and the Cys residue of CDR1. Human origin protein structures with the same orientation were searched in PDB and ubiquitin was selected. Ubi‐(1RI8), the CDR3‐grafted ubiquitin, had antigen‐binding ability, though the affinity was relatively low compared to cAb‐CA05‐(1RI8). The thermodynamic parameters of Ubi‐(1RI8) binding to HEWL were different from cAb‐CA05‐(1RI8). Hydrogen‐deuterium exchange experiments showed decreased stability around the CDR3 grafting region of Ubi‐(1RI8), which might explain the decreased antigen‐binding ability and the differences in thermodynamic properties. We concluded that the orientation of the CDR3 sequence of Ubi‐(1RI8) could not be reconstructed correctly.


Acta Crystallographica Section D-biological Crystallography | 2002

Crystallization of the functional domain of human thrombopoietin using an antigen-binding fragment derived from neutralizing monoclonal antibody

Ryota Kuroki; Masako Hirose; Yoichi Kato; Michael D. Feese; Taro Tamada; Hideki Shigematsu; Hiroshi Watarai; Yoshitake Maeda; Tomoyuki Tahara; Takashi Kato; Hiroshi Miyazaki

Thrombopoietin (TPO) is a cytokine which primarily stimulates megakaryocytopoiesis and thrombopoiesis. The functional domain of TPO (TPO(163)) consisting of the N-terminal 163 amino acids was prepared and crystallized. Since the crystallization of TPO(163) was unsuccessful using the standard screening methods, a Fab fragment derived from a neutralizing monoclonal antibody was used for crystallization. It was found that the TPO(163)-Fab complex crystallized reproducibly in 0.1 M potassium phosphate buffer pH 6.0 containing 20-25% polyethylene glycol 4000. Thin crystals (0.2 x 0.2 x 0.02 mm) grew in two space groups: P2(1), with unit-cell parameters a = 133.20, b = 46.71, c = 191.47 A, beta = 90.24 degrees, and C2, with unit-cell parameters a = 131.71, b = 46.48, c = 184.63 A, beta = 90.42 degrees. The results of a molecular-replacement analysis indicate that the Fab molecules interact with each other and provide a suitable interface for crystallization.


Acta Crystallographica Section F-structural Biology and Crystallization Communications | 2005

Crystallization of a 2:2 complex of granulocyte-colony stimulating factor (GCSF) with the ligand-binding region of the GCSF receptor.

Eijiro Honjo; Taro Tamada; Yoshitake Maeda; Takumi Koshiba; Yasuko Matsukura; Tomoyuki Okamoto; Matsujiro Ishibashi; Masao Tokunaga; Ryota Kuroki

The granulocyte-colony stimulating factor (GCSF) receptor receives signals for regulating the maturation, proliferation and differentiation of the precursor cells of neutrophilic granulocytes. The signalling complex composed of two GCSFs (GCSF, 19 kDa) and two GCSF receptors (GCSFR, 34 kDa) consisting of an Ig-like domain and a cytokine-receptor homologous (CRH) domain was crystallized. A crystal of the complex was grown in 1.0 M sodium formate and 0.1 M sodium acetate pH 4.6 and belongs to space group P4(1)2(1)2 (or its enantiomorph P4(3)2(1)2), with unit-cell parameters a = b = 110.1, c = 331.8 A. Unfortunately, this crystal form did not diffract beyond 5 A resolution. Since the heterogeneity of GCSF receptor appeared to prevent the growth of good-quality crystals, the GCSF receptor was fractionated by anion-exchange chromatography. Crystals of the GCSF-fractionated GCSF receptor complex were grown as a new crystal form in 0.2 M ammonium phosphate. This new crystal form diffracted to beyond 3.0 A resolution and belonged to space group P3(1)21 (or its enantiomorph P3(2)21), with unit-cell parameters a = b = 134.8, c = 105.7 A.


Protein Science | 2016

An insight into the thermodynamic characteristics of human thrombopoietin complexation with TN1 antibody.

Shigeki Arai; Chie Shibazaki; Motoyasu Adachi; Eijiro Honjo; Taro Tamada; Yoshitake Maeda; Tomoyuki Tahara; Takashi Kato; Hiroshi Miyazaki; Michael Blaber; Ryota Kuroki

Human thrombopoietin (hTPO) primarily stimulates megakaryocytopoiesis and platelet production and is neutralized by the mouse TN1 antibody. The thermodynamic characteristics of TN1 antibody–hTPO complexation were analyzed by isothermal titration calorimetry (ITC) using an antigen‐binding fragment (Fab) derived from the TN1 antibody (TN1‐Fab). To clarify the mechanism by which hTPO is recognized by TN1‐Fab the conformation of free TN1‐Fab was determined to a resolution of 2.0 Å using X‐ray crystallography and compared with the hTPO‐bound form of TN1‐Fab determined by a previous study. This structural comparison revealed that the conformation of TN1‐Fab does not substantially change after hTPO binding and a set of 15 water molecules is released from the antigen‐binding site (paratope) of TN1‐Fab upon hTPO complexation. Interestingly, the heat capacity change (ΔCp) measured by ITC (−1.52 ± 0.05 kJ mol−1 K−1) differed significantly from calculations based upon the X‐ray structure data of the hTPO‐bound and unbound forms of TN1‐Fab (−1.02 ∼ 0.25 kJ mol−1 K−1) suggesting that hTPO undergoes an induced‐fit conformational change combined with significant desolvation upon TN1‐Fab binding. The results shed light on the structural biology associated with neutralizing antibody recognition.


Proceedings of the National Academy of Sciences of the United States of America | 2006

Homodimeric cross-over structure of the human granulocyte colony-stimulating factor (GCSF) receptor signaling complex

Taro Tamada; Eijiro Honjo; Yoshitake Maeda; Tomoyuki Okamoto; Matsujiro Ishibashi; Masao Tokunaga; Ryota Kuroki


Biochemistry | 2004

Thermodynamic analysis of the activation mechanism of the GCSF receptor induced by ligand binding.

Shouhei Mine; Takumi Koshiba; Eijiro Honjo; Tomoyuki Okamoto; Taro Tamada; Yoshitake Maeda; Yasuko Matsukura; Akane Horie; Matsujiro Ishibashi; Miharu Sato; Mizue Azuma; Masao Tokunaga; Katsutoshi Nitta; Ryota Kuroki


Seibutsu Butsuri | 2007

3P003 Structural and Thermodynamic Change upon the Antigen Binding of Human Thrombopoietin Neutralizing IgG TN1(Proteins-structure and structure-function relationship,Poster Presentations)

Shigeki Arai; Taro Tamada; Yoshitake Maeda; Ryota Kuroki

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Ryota Kuroki

Japan Atomic Energy Agency

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Taro Tamada

Japan Atomic Energy Agency

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Eijiro Honjo

Japan Atomic Energy Agency

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Masako Hirose

National Institute of Advanced Industrial Science and Technology

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Shigeki Arai

Japan Atomic Energy Agency

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