Yoshitami Ohashi
Meijo University
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Featured researches published by Yoshitami Ohashi.
The Journal of Antibiotics | 2011
Azusa Kato; Haruhisa Hirata; Yoshitami Ohashi; Kiyonaga Fujii; Kenji Mori; Ken-ichi Harada
The anti-MRSA antibiotic, WAP-8294A, was isolated from the fermentation broth of Lysobacter sp. The major component, WAP-8294A2, is composed of 1 mol of Gly, L-Leu, L-Glu, D-Asn, D-Trp, D-threo-β-hydroxyasparagine, N-Me-D-Phe and N-Me-L-Val, and 2 mol of L-Ser, D-Orn and D-3-hydroxy-7-Me-octanoic acid. The structure of the WAP-8294A2 was mainly determined as a cyclic depsipeptide by 2D NMR experiments. However, it was difficult to use the NMR experiment to determine the minor components, A1, A4 and Ax13, isolated in small amounts. In the present study, ESI MS/MS was applied to the structure elucidation of these minor components. The structures of these minor components were determined on the basis of the fragmentation pattern of the product ions of WAP-8294A2 in the ESI MS/MS. As a result, it was confirmed that A1 and A4 had the same amino acid sequence as A2, while A1 and A4 had the 3-OH-octanoic acid and 3-OH-8-Me-nonanoic acid, respectively, in the place of the 3-OH-7-Me-octanoic acid in A2. In the structure of Ax13, it was found that Gly of A2 was changed to β-Ala of Ax13.
The Journal of Antibiotics | 1998
Azusa Kato; Seigo Nakaya; Naomi Kokubo; Yuji Aiba; Yoshitami Ohashi; Haruhisa Hirata; Kiyonaga Fujii; Ken-ichi Harada
The Journal of Antibiotics | 1968
Yukio Ito; Yoshitami Ohashi; Yutaka Sakurai; Masaharu Sakurazawa; Hironaga Yoshida; Shigemi Awataguchi; Tomoharu Okuda
Archive | 1996
Takeshi Aiba; Haruhisa Hirata; Shigeru Kamiya; Yasuhiro Koga; Naomi Kokubo; Makoto Maeda; Yoshitami Ohashi; Nobuyuki Suzuki; 孚 前田; 泰裕 古賀; 良民 大橋; 直美 小久保; 晴久 平田; 勇志 相場; 茂 神谷; 信之 鈴木
The Journal of Antibiotics | 1978
Katsuo Takeda; Satoshi Okuno; Yoshitami Ohashi; Tamotsu Furumai
Carbohydrate Research | 1978
Yoshitami Ohashi; Satoshi Okuno; Katsuo Takeda; Yukio Ito
Archive | 1994
Makoto Maeda; Eriko Matsuyama; Ritsuko Mochida; Yoshitami Ohashi; Hirokazu Suzuki; Masahiko Wakasugi; 孚 前田; 良民 大橋; 立子 持田; 恵理子 松山; 昌彦 若杉; 宏和 鈴木
The Journal of Antibiotics | 1973
Yukio Ito; Yoshitami Ohashi; Shuzo Kawabe; Masaharu Sakurazawa; Toshio Ogawa; Yoshiyuki Egawa; Tomoharu Okuda
Archive | 1995
Takeshi Aiba; Yoshio Hida; Haruhisa Hirata; Yasuhiro Koga; Naomi Kokubo; Makoto Maeda; Yoshitami Ohashi; Nobuyuki Suzuki; 孚 前田; 泰裕 古賀; 良民 大橋; 直美 小久保; 晴久 平田; 勇志 相場; 信之 鈴木; 美穂 飛田
Agricultural and biological chemistry | 1973
Yoshitami Ohashi; Hiroshi Abe; Yukio Ito