Young-Hwan Moon
Chonnam National University
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Publication
Featured researches published by Young-Hwan Moon.
Biotechnology and Bioprocess Engineering | 2012
Thi Thanh Hanh Nguyen; Sun-Hwa Jung; Sun Lee; Hwa-Ja Ryu; Hee-Kyoung Kang; Young-Hwan Moon; Young-Min Kim; Atsuo Kimura; Doman Kim
Human intestinal maltase (HMA) is an α-glucosidase responsible for the hydrolysis of α-1,4-linkages from the non-reducing end of malto-oligosaccharides. HMA has become an important target in the treatment of type-2 diabetes. In this study, epigallocatechin gallate (EGCG) and EGCG glucoside (EGCG-G1) were identified as inhibitors of HMA by an in vitro assay with IC50 of 20 ± 1.0 and 31.5 ± 1.0 μM, respectively. A Lineweaver-Burk plot confirmed that EGCG and EGCG-G1 were competitive inhibitors of maltose substrate against HMA and inhibition kinetic constants (Ki) calculated from a Dixon plot were 5.93 ± 0.26 and 7.88 ± 0.57 μM, respectively. Both EGCG and EGCG-G1 bound to the active site of HMA with numerous hydrophobic and hydrogen bond interactions.
Enzyme and Microbial Technology | 2013
Thi Thanh Hanh Nguyen; Young-Hwan Moon; Young-Bae Ryu; Young-Min Kim; Seung-Hee Nam; Misook Kim; Atsuo Kimura; Doman Kim
The human fibroblast collagenase catalytic domain (MMP1ca) that is considered a prototype for all interstitial collagenase and plays an important role in the turnover of collagen fibrils in the matrix was expressed as an inclusion body in the Escherichia coli. The purified enzyme displayed activity with substrate Dnp-Pro-Leu-Ala-Leu-Trp-Ala-Arg-OH with a K(m) value of 26.61±1.42 μM. The inhibition activity of the nine flavonoid compounds and gallic acid against MMP1ca was examined. Among the compounds tested, the IC(50) of seven flavonoid compounds were determined and ranged from 14.13 to 339.21 μM. Epigallocatechin gallate (EGCG) showed the highest inhibition toward MMP1ca with IC(50) values of 14.13±0.49 μM. EGCG showed a competitive inhibition pattern with a K(i) value of 10.47±0.51 μM. The free binding energy of EGCG against MMP1ca was -13.07 kcal mol(-1), which was calculated by using Autodock 3.0.5 software and showed numerous hydrophobic and hydrogen bond interactions. The galloyl group of EGCG, gallocatechin gallate and epicatechin gallate was determined to be important for inhibitory activity against MMP1ca.
Journal of Agricultural and Food Chemistry | 2006
Young-Hwan Moon; Jin-Ha Lee; Joon-Seob Ahn; Seung-Hee Nam; Deok-Kun Oh; Don-Hee Park; Hyun-Ju Chung; Seong-Soo Kang; Donal F. Day; Doman Kim
Enzyme and Microbial Technology | 2012
Hye-Jin Woo; Hee-Kyoung Kang; Thi Thanh Hanh Nguyen; Go-Eun Kim; Young-Min Kim; Jun-Seong Park; Duwoon Kim; Jaeho Cha; Young-Hwan Moon; Seung-Hee Nam; Yongmei Xia; Atsuo Kimura; Doman Kim
Enzyme and Microbial Technology | 2007
Young-Hwan Moon; Jin-Ha Lee; Deok-Young Jhon; Woojin Jun; Seong-Soo Kang; Jeonggu Sim; Heungsic Choi; Jae-Hak Moon; Doman Kim
Enzyme and Microbial Technology | 2006
Jin-Ha Lee; Seung-Heon Yoon; Seung-Hee Nam; Young-Hwan Moon; You-Youn Moon; Doman Kim
Journal of Molecular Catalysis B-enzymatic | 2006
Young-Hwan Moon; Ghahyun J. Kim; Jin-Ha Lee; Xing-Ji Jin; Do-Won Kim; Doman Kim
Biotechnology Letters | 2008
Jin-Ha Lee; Young-Hwan Moon; Nahyun Kim; Young-Min Kim; Hee-Kyoung Kang; Ji-Yeon Jung; Emad Abada; Seong-Soo Kang; Doman Kim
Fems Microbiology Letters | 2006
Jin-Ha Lee; Hee-Kyoung Kang; Young-Hwan Moon; Dong Lyun Cho; Doman Kim; Jun-Yong Choe; R. Honzatko; John F. Robyt
Journal of Agricultural and Food Chemistry | 2016
Jiyoun Kim; Thi Thanh Hanh Nguyen; Nahyun M. Kim; Young-Hwan Moon; Jungmin Ha; Namhyeon Park; DongGu Lee; Kyeonghwan Hwang; Jun-Seong Park; Doman Kim