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Dive into the research topics where Young-Hwan Moon is active.

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Featured researches published by Young-Hwan Moon.


Biotechnology and Bioprocess Engineering | 2012

Inhibitory effects of epigallocatechin gallate and its glucoside on the human intestinal maltase inhibition

Thi Thanh Hanh Nguyen; Sun-Hwa Jung; Sun Lee; Hwa-Ja Ryu; Hee-Kyoung Kang; Young-Hwan Moon; Young-Min Kim; Atsuo Kimura; Doman Kim

Human intestinal maltase (HMA) is an α-glucosidase responsible for the hydrolysis of α-1,4-linkages from the non-reducing end of malto-oligosaccharides. HMA has become an important target in the treatment of type-2 diabetes. In this study, epigallocatechin gallate (EGCG) and EGCG glucoside (EGCG-G1) were identified as inhibitors of HMA by an in vitro assay with IC50 of 20 ± 1.0 and 31.5 ± 1.0 μM, respectively. A Lineweaver-Burk plot confirmed that EGCG and EGCG-G1 were competitive inhibitors of maltose substrate against HMA and inhibition kinetic constants (Ki) calculated from a Dixon plot were 5.93 ± 0.26 and 7.88 ± 0.57 μM, respectively. Both EGCG and EGCG-G1 bound to the active site of HMA with numerous hydrophobic and hydrogen bond interactions.


Enzyme and Microbial Technology | 2013

The influence of flavonoid compounds on the in vitro inhibition study of a human fibroblast collagenase catalytic domain expressed in E. coli.

Thi Thanh Hanh Nguyen; Young-Hwan Moon; Young-Bae Ryu; Young-Min Kim; Seung-Hee Nam; Misook Kim; Atsuo Kimura; Doman Kim

The human fibroblast collagenase catalytic domain (MMP1ca) that is considered a prototype for all interstitial collagenase and plays an important role in the turnover of collagen fibrils in the matrix was expressed as an inclusion body in the Escherichia coli. The purified enzyme displayed activity with substrate Dnp-Pro-Leu-Ala-Leu-Trp-Ala-Arg-OH with a K(m) value of 26.61±1.42 μM. The inhibition activity of the nine flavonoid compounds and gallic acid against MMP1ca was examined. Among the compounds tested, the IC(50) of seven flavonoid compounds were determined and ranged from 14.13 to 339.21 μM. Epigallocatechin gallate (EGCG) showed the highest inhibition toward MMP1ca with IC(50) values of 14.13±0.49 μM. EGCG showed a competitive inhibition pattern with a K(i) value of 10.47±0.51 μM. The free binding energy of EGCG against MMP1ca was -13.07 kcal mol(-1), which was calculated by using Autodock 3.0.5 software and showed numerous hydrophobic and hydrogen bond interactions. The galloyl group of EGCG, gallocatechin gallate and epicatechin gallate was determined to be important for inhibitory activity against MMP1ca.


Journal of Agricultural and Food Chemistry | 2006

Synthesis, Structure Analyses, and Characterization of Novel Epigallocatechin Gallate (EGCG) Glycosides Using the Glucansucrase from Leuconostoc mesenteroides B-1299CB

Young-Hwan Moon; Jin-Ha Lee; Joon-Seob Ahn; Seung-Hee Nam; Deok-Kun Oh; Don-Hee Park; Hyun-Ju Chung; Seong-Soo Kang; Donal F. Day; Doman Kim


Enzyme and Microbial Technology | 2012

Synthesis and characterization of ampelopsin glucosides using dextransucrase from Leuconostoc mesenteroides B-1299CB4: Glucosylation enhancing physicochemical properties

Hye-Jin Woo; Hee-Kyoung Kang; Thi Thanh Hanh Nguyen; Go-Eun Kim; Young-Min Kim; Jun-Seong Park; Duwoon Kim; Jaeho Cha; Young-Hwan Moon; Seung-Hee Nam; Yongmei Xia; Atsuo Kimura; Doman Kim


Enzyme and Microbial Technology | 2007

Synthesis and characterization of novel quercetin-α-d-glucopyranosides using glucansucrase from Leuconostoc mesenteroides

Young-Hwan Moon; Jin-Ha Lee; Deok-Young Jhon; Woojin Jun; Seong-Soo Kang; Jeonggu Sim; Heungsic Choi; Jae-Hak Moon; Doman Kim


Enzyme and Microbial Technology | 2006

Molecular cloning of a gene encoding the sucrose phosphorylase from Leuconostoc mesenteroides B-1149 and the expression in Escherichia coli

Jin-Ha Lee; Seung-Heon Yoon; Seung-Hee Nam; Young-Hwan Moon; You-Youn Moon; Doman Kim


Journal of Molecular Catalysis B-enzymatic | 2006

Enzymatic synthesis and characterization of novel epigallocatechin gallate glucosides

Young-Hwan Moon; Ghahyun J. Kim; Jin-Ha Lee; Xing-Ji Jin; Do-Won Kim; Doman Kim


Biotechnology Letters | 2008

Cloning and expression of the sucrose phosphorylase gene from Leuconostoc mesenteroides in Escherichia coli

Jin-Ha Lee; Young-Hwan Moon; Nahyun Kim; Young-Min Kim; Hee-Kyoung Kang; Ji-Yeon Jung; Emad Abada; Seong-Soo Kang; Doman Kim


Fems Microbiology Letters | 2006

Cloning, expression and characterization of an extracellular enolase from Leuconostoc mesenteroides

Jin-Ha Lee; Hee-Kyoung Kang; Young-Hwan Moon; Dong Lyun Cho; Doman Kim; Jun-Yong Choe; R. Honzatko; John F. Robyt


Journal of Agricultural and Food Chemistry | 2016

Functional Properties of Novel Epigallocatechin Gallate Glucosides Synthesized by Using Dextransucrase from Leuconostoc mesenteroides B-1299CB4

Jiyoun Kim; Thi Thanh Hanh Nguyen; Nahyun M. Kim; Young-Hwan Moon; Jungmin Ha; Namhyeon Park; DongGu Lee; Kyeonghwan Hwang; Jun-Seong Park; Doman Kim

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Doman Kim

Seoul National University

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Jin-Ha Lee

Chonnam National University

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Seung-Hee Nam

Chonnam National University

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Young-Min Kim

Chonnam National University

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Hee-Kyoung Kang

Chonnam National University

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Seong-Soo Kang

Chonnam National University

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