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Dive into the research topics where Yu-Chan Chao is active.

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Featured researches published by Yu-Chan Chao.


Biotechnology Progress | 2008

Rapid titer determination of baculovirus by quantitative real-time polymerase chain reaction.

Huei-Ru Lo; Yu-Chan Chao

Titer determination is a prerequisite for the study of viruses. However, the current available methods are tedious and time‐consuming. To improve the efficiency of titer determination, we have developed a rapid and simple method for the routine detection of baculovirus titers using a quantitative real‐time PCR. This method is based on the amplification of approximately 150‐bp fragments located in the coding regions of selected genes. The PCR was found to be quantitative in a range of 103 to 109 virus particles per 200 μL of supernatant, and the results were closely correlated with titers detected from 50% tissue culture infectious doses (TCID50) of baculovirus. This quantitative real‐time PCR requires only 30 min to perform, and the entire titer determination can be accomplished within 1 h without the need for cell seeding or further virus dilution and infection. Because this technology is easy to operate, generates data with high precision, and most importantly is very quick, it will certainly be broadly applied for titer determination of baculoviruses in the future.


Biochemical and Biophysical Research Communications | 2004

Assembly of human severe acute respiratory syndrome coronavirus-like particles

Yu Ho; Pi-Hsiu Lin; Catherine Yen-Yen Liu; Su-Ping Lee; Yu-Chan Chao

Abstract Viral particles of human severe acute respiratory syndrome coronavirus (SARS CoV) consist of three virion structural proteins, including spike protein, membrane protein, and envelope protein. In this report, virus-like particles were assembled in insect cells by the co-infection with recombinant baculoviruses, which separately express one of these three virion proteins. We found that the membrane and envelope proteins are sufficient for the efficient formation of virus-like particles and could be visualized by electron microscopy. Sucrose gradient purification followed by Western blot analysis and immunogold labeling showed that the spike protein could be incorporated into the virus like particle also. The construction of engineered virus-like particles bearing resemblance to the authentic one is an important step towards the development of an effective vaccine against infection of SARS CoV.


Journal of Immunology | 2004

Induction of IL-8 Release in Lung Cells via Activator Protein-1 by Recombinant Baculovirus Displaying Severe Acute Respiratory Syndrome-Coronavirus Spike Proteins: Identification of Two Functional Regions

Ya-Jen Chang; Catherine Yen-Yen Liu; Bor-Luen Chiang; Yu-Chan Chao; Ching-Chow Chen

The inflammatory response and the intracellular signaling pathway induced by severe acute respiratory syndrome (SARS)-coronavirus (CoV) were studied in lung epithelial cells and fibroblasts. SARS-CoV spike (S) protein-encoding plasmid induced activations of IL-8 promoter and AP-1, but not NF-κB in these cells. Mutation of the AP-1, not the κB site, abolished the SARS-CoV S protein-induced IL-8 promoter activity. IL-8 release was effectively induced by vAtEpGS688, a baculovirus exhibiting the aa 17–688 fragment of S protein, and this induction was attenuated by the angiotensin-converting enzyme 2 Ab. Recombinant baculovirus expressing different deletion and insertion fragments identified the functional region of S protein from aa 324–688 (particularly the N-terminal aa 324–488 and the C-terminal aa 609–688), which is responsible for IL-8 production. Activations of AP-1 DNA-protein binding and MAPKs after vAtEpGS688 transduction were demonstrated, and SARS-CoV S protein-induced IL-8 promoter activity was inhibited by the specific inhibitors of MAPK cascades. These results suggested that the S protein of SARS-CoV could induce release of IL-8 in the lung cells via activations of MAPKs and AP-1. The identification of the functional domain for IL-8 release will provide for the drug design on targeting specific sequence domains of S protein responsible for initiating the inflammatory response.


Journal of Biological Chemistry | 2002

Novel Baculovirus DNA Elements Strongly Stimulate Activities of Exogenous and Endogenous Promoters

Huei-Ru Lo; Cheng-Chung Chou; Tzong-Yuan Wu; Joyce Pui-Yee Yuen; Yu-Chan Chao

A DNA sequence upstream from the polyhedrin gene of baculovirus Autographa californicanucleopolyhedrovirus (AcMNPV) was found to activate strongly the expression of full or minimal promoters derived from AcMNPV and other sources. Promoters tested included the minimal CMV (CMVm) promoter from human cytomegalovirus, the full heat shock 70 promoter fromDrosophila, and the minimal p35 promoter from baculovirus. Deletion and mutagenesis analyses showed that this functional polyhedrin upstream (pu) activator sequence contains three open reading frames (ORFs), ORF4, ORF5, andlef2. In plasmid transfection assays, the pusequence was able to confer high level luciferase expression driven by all of these full or minimal promoters in insect Sf21 cells. A known baculovirus enhancer, the homologous region (hr) of AcMNPV, further enhanced the expression of these promoters. Experiments showed that although multiple hr sequences function in an additive manner, pu and hr together function synergistically, resulting in as much as 18,000-fold promoter activation. Furthermore, a modified CMVm promoter containingpu and/or hr was inserted into the baculovirus genome to drive the luciferase coding region. The CMVm promoter expressed luciferase much earlier, and although it expressed a bit less than did the p10 promoter, the CMVm promoter gave rise to greater luciferase activity. Therefore, we have uncovered a cryptic viral sequence capable of activating a diverse group of promoters. Finally, these experiments demonstrate that synthetic sequences containing pu, hr, and different full or minimal promoters can generate a set of essentially unlimited novel promoters for weak to very strong expression of foreign proteins using baculovirus.


Scientific Reports | 2011

A Non-coding RNA of Insect HzNV-1 Virus Establishes Latent Viral Infection through MicroRNA

Yueh-Lung Wu; Carol P. Wu; Catherine Yen-Yen Liu; Paul Wei-Che Hsu; Eric C. Wu; Yu-Chan Chao

Heliothis zea nudivirus-1 (HzNV-1) is an insect virus previously known as Hz-1 baculovirus. One of its major early genes, hhi1, is responsible for the establishment of productive viral infection; another gene, pag1, which expresses a non-coding RNA, is the only viral transcript detectable during viral latency. Here we showed that this non-coding RNA was further processed into at least two distinct miRNAs, which targeted and degraded hhi1 transcript. This is a result strikingly similar to a recent report that herpes simplex virus produces tightly-regulated latent specific miRNAs to silence its own key early transcripts. Nevertheless, proof for the establishment of viral latency by miRNA is still lacking. We further showed that HzNV-1 latency could be directly induced by pag1-derived miRNAs in cells infected with a pag1-deleted, latency-deficient virus. This result suggests the existence of a novel mechanism, where miRNAs can be functional for the establishment of viral latency.


Current Gene Therapy | 2010

Maximizing Baculovirus-Mediated Foreign Proteins Expression in Mammalian Cells

Catherine Yen-Yen Liu; Hong-Zhang Chen; Yu-Chan Chao

Since the discovery of mammalian cells susceptible to baculovirus transduction in 1995, baculovirus has emerged as a safe, economical and convenient tool for introducing foreign genetic material into mammalian systems. The feasibility of baculovirus as a novel gene transfer vehicle for large scale mammalian protein expression was not obvious at first, as the baculovirus genome is unable to amplify within mammalian cells and expression of the transferred foreign gene does not usually last more than 20 days. However, several recent articles have demonstrated that baculovirus contains both the elements - hr sequences, and the factor - IE2 protein, which can function in mammalian cells to dramatically boost baculovirus-transduced gene expression. The longevity of genes transferred by baculovirus can also be extended by inserting a replication ori with the necessary factors and induce self-replicating episomes. Lastly, peptide-based baculovirus surface modification and two groups of drugs: histone deacetylase inhibitors and microtubule depolymerizing agents, which improve baculovirus gene expression were described. In this article we summarized ways to activate, enhance, and prolong baculovirus-mediated foreign protein expression. The mechanism behind IE2 activation through its unique nuclear body structure was given special emphasis, including our most current data which suggested PML NBs could be disrupted by IE2. Finally, strategies to maximizing mammalian protein production through baculovirus gene transfer are proposed in prospect to current breakthroughs.


Biochemical Journal | 2004

Enhancement of correct protein folding in vivo by a non-lytic baculovirus.

Yu Ho; Huei-Ru Lo; Tzu-Ching Lee; Carol P. Wu; Yu-Chan Chao

The BEVS (baculovirus expression vector system) is widely used for the production of proteins. However, engineered proteins frequently experience the problem of degradation, possibly due to the lytic nature of the conventional BEVS (herein referred to as L-BEVS). In the present study, a non-lytic BEVS (N-BEVS) was established by random mutagenesis of viral genomes. At 5 days post-infection, N-BEVS showed only 7% cell lysis, whereas L-BEVS showed 60% lysis of cells. The quality of protein expressed in both N- and L-BEVSs was examined further using a novel FRET (fluorescence resonance energy transfer)-based assay. To achieve this, we constructed a concatenated fusion protein comprising LUC (luciferase) sandwiched between EYFP (enhanced yellow fluorescent protein) and ECFP (enhanced cyan fluorescent protein). The distance separating the two fluorescent proteins in the fusion protein EYFP-LUC-ECFP (designated hereafter as the YLC construct) governs energy transfer between EYFP and ECFP. FRET efficiency thus reflects the compactness of LUC, indicating its folding status. We found more efficient FRET in N-BEVS compared with that obtained in L-BEVS, suggesting that more tightly folded LUC was produced in N-BEVS. YLC expression was also analysed by Western blotting, revealing significantly less protein degradation in N-BEVS than in L-BEVS, in which extensive degradation was observed. This FRET-based in vivo folding technology showed that YLC produced in N-BEVS is more compact, correlating with improved resistance to degradation. N-BEVS is thus a convenient alternative for L-BEVS for the production of proteins vulnerable to degradation using baculoviruses.


Journal of Biotechnology | 2000

Expression of highly controllable genes in insect cells using a modified tetracycline-regulated gene expression system

Tzong-Yuan Wu; Lina Liono; Su-Liang Chen; Chin-Yu Chen; Yu-Chan Chao

A modified tetracycline-responsive expression system (TRES) for use in insect cells was developed. The TRES contains two components: one encodes a tetracycline-controllable transactivator (tTA) and the other contains a tet operator DNA sequence to drive the luciferase gene. Our results show that the human cytomegalovirus (CMV) promoter, an essential part for strong tTA expression in mammalian system, was not functional in insect cells. Thus further modifications were required. Functional tTA was efficiently expressed in Sf9, Sf21, and TN368 cells by the p10 promoter of Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) in plasmid form with virus co-infection. An increase of up to 258-fold of luciferase activity was detected in these cells when both components in modified TRES were co-transfected. In order to further simplify the experiment, tTA, which is driven by the p10 promoter, was inserted into AcMNPV. Luciferase activity was also strongly stimulated by the infection of this tTA expression-recombinant virus with the transfection of a plasmid containing the second TRES component expressing luciferase. The luciferase expressions in these systems, either in plasmids or the tTA gene in virus and luciferase in plasmid, were significantly suppressed by tetracycline. The time course kinetics of tetracycline action to the TRES were further studied. Within a time span of 50 h, the luciferase activities could be fully suppressed or activated, respectively, corresponding to the addition or removal of tetracycline. These experiments have established a well-regulated gene expression system for further broad applications of molecular biological studies in insect cells.


Journal of Virology | 2010

The Early Gene hhi1 Reactivates Heliothis zea Nudivirus 1 in Latently Infected Cells

Yueh-Lung Wu; Carol P. Wu; Song Tay Lee; Han Tang; Chi Hua Chang; Hong Hwa Chen; Yu-Chan Chao

ABSTRACT Heliothis zea nudivirus 1 (HzNV-1), previously known as Hz-1 virus, is an insect virus able to establish both productive and latent infections in several lepidopteran insect cells. Here, we have cloned and characterized one of the HzNV-1 early genes, hhi1, which maps to the HindIII-I fragment of the viral genome. During the productive viral infection, a 6.2-kb hhi1 transcript was detectable as early as 0.5 h postinfection (hpi). The level of transcript reached a maximum at 2 hpi and gradually decreased after 4 hpi. The transcript was not detectable during the latent phase of viral infection. Upon cycloheximide treatment, much higher levels of hhi1 transcript were detected throughout the productive viral infection cycle, suggesting that newly synthesized proteins are not needed for the expression of hhi1. Nevertheless, viral coinfection can further stimulate the expression of transfected hhi1 promoter in a plasmid. Transient hhi1 expression in latently infected cells resulted in a significant increase in virus titer and viral DNA propagation, suggesting that hhi1 plays a critical role in viral reactivation. Additional experiments showed that six early genes, which possibly function in transcription or DNA replication, were activated in the latent cells upon hhi1 transfection. Among these six genes, orf90 and orf121 expression could be induced by hhi1 alone without the need for other viral genes. Our discovery should be useful for future mechanistic study of the switches of latent/productive HzNV-1 viral infections.


Journal of Virology | 2009

RING and Coiled-Coil Domains of Baculovirus IE2 Are Critical in Strong Activation of the Cytomegalovirus Major Immediate-Early Promoter in Mammalian Cells

Catherine Yen-Yen Liu; Chia-Hung Wang; Wen-Kai Hsiao; Huei-Ru Lo; Carol P. Wu; Yu-Chan Chao

ABSTRACT In recent years, baculovirus has emerged as a tool for high-efficiency gene transfer into mammalian cells. However, the level of gene expression is often limited by the strength of the mammalian promoter used. Here, we show that the baculovirus RING protein IE2 is a strong, promiscuous trans-activator in mammalian cells, dramatically upregulating the cytomegalovirus (CMV) promoter in both Vero E6 and U-2OS cells. Further study of the cellular mechanism for the activation led to the discovery of a novel IE2 nuclear body structure which contains a high concentration of G-actin and closely associates with RNA polymerase II, PML, and SUMO1. IE2 mutagenesis studies indicated that the RING and coiled-coil domains of IE2 were necessary for nuclear body formation, as well as for strong activation of the CMV promoter in mammalian cells. Overall, this study shows that the IE2 trans-activator could significantly advance the use of baculovirus in mammalian gene transfer and protein production.

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