Yu. V. Khramova
Moscow State University
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Featured researches published by Yu. V. Khramova.
Zygote | 2016
M. A. Filatov; Yu. V. Khramova; M.V. Kiseleva; I.V. Malinova; E.V. Komarova; M. L. Semenova
In the present review, the main strategies of female fertility preservation are covered. Procedures of fertility preservation are necessary for women who suffer from diseases whose treatment requires the use of aggressive therapies, such as chemotherapy and radiotherapy. These kinds of therapy negatively influence the health of gametes and their progenitors. The most commonly used method of female fertility preservation is ovarian tissue cryopreservation, followed by the retransplantation of thawed tissue. Another approach to female fertility preservation that has been actively developed lately is the ovarian tissue in vitro culture. The principal methods, advantages and drawbacks of these two strategies are discussed in this article.
High Temperature | 2015
I. V. Ilina; Yu. V. Khramova; M. A. Filatov; M. L. Semenova; D. S. Sitnikov
We demonstrate that one of the key steps of preimplantation genetic diagnosis called embryo biopsy can be successfully performed in noncontact mode by means of femtosecond laser scalpel and optical tweezers. Embryo biopsy was carried out in late-stage mouse preimplantation embryos. Femtosecond laser pulses were applied to detach the desired amount of trophectoderm cells from the blastocyst, while the optical tweezers trapped the cells and moved them out of the embryo. The parameters of laser radiation were optimized so as to efficiently perform embryo biopsy and preserve the viability of the treated embryos.
High Temperature | 2013
I. V. Ilina; A. V. Ovchinnikov; D. S. Sitnikov; M.M. Rakityanskiy; M. B. Agranat; Yu. V. Khramova; M. L. Semenova
The results are presented of the works in the field of development of equipment, investigation techniques, and technologies for biology and medicine performed in the Joint Institute for High Temperatures of the Russian Academy of Scienses (JIHT RAS). On the base of the new generation infrared femtosecond lasers, the experimental models are developed and manufactured of laser tweezers, scalpel, and the “tweezers-scalpel” combined system. The results are presented of the experimental studies on the noncontact mammalian cell fusion (blastomeres of mouse embryos on day 1.5 of development) by means of the femtosecond laser pulses.
High Temperature | 2016
I. V. Ilina; Yu. V. Khramova; M. A. Filatov; M. L. Semenova; D. S. Sitnikov
Ultrashort laser pulses have enabled highly precise and delicate processing of biological specimens. We present the results of using femtosecond laser pulses for dissection of zona pellucida (ZP) in mouse embryos during assisted hatching procedure. We studied the effects of application of femtosecond laser radiation in the infrared (1028 nm) and visible (514 nm) wavelength ranges. Laser irradiation parameters were optimized so as not to compromise the viability of the treated embryos. We have demonstrated that application of femtosecond laser pulses with the energies in the range of 250–320 nJ (for the wavelength of 1028 nm) and 47–112 nJ (for 514 nm) resulted in efficient ZP dissection. Femtosecond laser-assisted ZP drilling does not slow down the development of pre-implantation embryos and leads to 90–95% frequency of complete hatching. The thermal effects can be significantly lower when femtosecond lasers are used as compared to continuous wave or long-pulse lasers. It is crucial when dealing with living cells or organisms. By optimizing femtosecond laser radiation parameters assisted hatching as well as a wide range of embryo-surgical procedures can be efficiently performed, thus creating a great potential of using femtosecond lasers as a multi-purpose “tool of choice” for specialists in the fields of embryology and developmental biology.
Russian Journal of Developmental Biology | 2018
D. A. Nikishin; Yu. V. Khramova; T. S. Bagayeva; M. L. Semenova; Yu. B. Shmukler
The functions of serotonin include the growth and development regulation of female germ cells as well as early embryo development. RT-PCR analysis of mRNA expression of the genes of the enzymes for synthesis and degradation and transporters and receptors of serotonin during folliculogenesis and preimplantation development of mice was performed to discover the particular mechanisms of these functions. The mRNA of tryptophan hydroxylase tph1 and monoaminoxidase maoa; membrane transporter sert and vesicular transporter vmat2; and serotonin receptors htr1b, htr1d, htr2a, htr5b, and htr7 were revealed in granulosa cells. The expression of mRNA of the aromatic amino acid decarboxylase ddc and the htr2b receptor additionally appears in the yellow body. The expression of mRNA of the genes of the tph2, ddc, and maoa enzymes; the sert, vmat1, and vmat2 transporters; and quite a number of receptors is observed during the preimplantation development, and it is transitory in most of them. The expression of all components and its dynamics suggest that the serotonergic signaling system is functionally active in mouse folliculogenesis and preimplantation development.
Journal of Physics: Conference Series | 2018
D. S. Sitnikov; I. V. Ilina; N. V. Kosheleva; Yu. V. Khramova; M. A. Filatov; M. L. Semenova; I.M. Zurina; A.A. Gorkun; Saburina In
Laser microsurgery has enabled us to make highly precise and delicate processing of living biological specimens. We present the results of using femtosecond (fs) laser pulses in assisted reproductive technologies. Femtosecond laser dissection of outer shells of embryos (so-called laser-assisted hatching) as well as laser-mediated detachment of the desired amount of trophectoderm cells (so-called embryo biopsy) required for preimplantaion genetic diagnosis were successfully performed. The parameters of laser radiation were optimized so as to efficiently perform embryo biopsy and preserve the viability of the treated embryos. Effects of application of fs-laser radiation in the infrared (1028 nm) and visible (514 nm) wavelength ranges were studied. We also applied laser microsurgery to develop a new simple reproducible model for studying repair and regeneration in vitro. Nanosecond laser pulses were applied to perform localized microdissection of cell spheroids. After microdissection, the edges of the wound surface opened, the destruction of the initial spheroid structure was observed in the wound area, with surviving cells changing their shape into a round one. It was shown that the spheroid form partially restored in the first six hours with subsequent complete restoration within seven days due to remodeling of surviving cells.
Moscow University Biological Sciences Bulletin | 2017
Elizaveta R. Pavlova; D. V. Bagrov; Yu. V. Khramova; Dmitry V. Klinov; K. V. Shaitan
Substrate topography influences cell shape, direction and rate of migration, nucleus shape, and gene expression levels. This influence is commonly studied using substrates with predefined surface structure and chemical composition. In the current work, we studied the state of HaCaT keratinocyte nuclei and actin cytoskeleton on poly(ε-caprolactone) scaffolds obtained by electrospinning. Two types of fibrous scaffolds were prepared and characterized. In the random scaffolds, the fibers were arranged in a nonsystematic fashion; however, most of the fibers had the same direction in the aligned scaffolds. When cultured on the aligned scaffolds, HaCaT cells exhibited oriented actin filaments and had more elongated nuclei.
Journal of Physics: Conference Series | 2016
D. S. Sitnikov; I. V. Ilina; Yu. V. Khramova; M. A. Filatov; M. L. Semenova
Ultrashort laser pulses have enabled highly precise and delicate processing of biological specimens. We present the results of using femtosecond (fs) laser pulses for dissection of zona pellucida (ZP) in mouse embryos during assisted hatching procedure and for trophectoderm biopsy as well. We studied the effects of application of fs laser radiation in the infrared (1028 nm) and visible (514 nm) wavelength ranges. Laser irradiation parameters were optimized so as not to compromise the viability of the treated embryos. Embryo biopsy was carried out in late-stage mouse preimplantation embryos. Femtosecond laser pulses were applied to detach the desired amount of trophectoderm cells from the blastocyst, while the optical tweezers trapped the cells and moved them out of the embryo. The parameters of laser radiation were optimized so as to efficiently perform embryo biopsy and preserve the viability of the treated embryos. The thermal effects can be significantly lower when fs lasers are used as compared to CW or long-pulse lasers. It is crucial when dealing with living cells or organisms.
ALT Proceedings | 2013
I. V. Ilina; O.V. Chefonov; M.B. Agranat; Yu. V. Khramova; A.V. Ovchinnikov; D. S. Sitnikov; M. L. Semenova
We report on results of using femtosecond laserscalpel for microsurgery of plasma membrane ofliving cells. Femtosecond laser pulses were appliedto initiate cell fusion as well as to performreversible permeabilization of cell membranerequired for efficient injection of extrinsicsubstances into the target cells. Laser-based cellfusion of mammalian embryo blastomeres as wellas fusion of cell bodies of neurons of molluskLymnaea stagnalis were successfully carried out byapplying single femtosecond laser pulses (secondharmonic of a Cr:Forsterite laser system) 620 nm,100 fs with the fluences of 0.42-0.71 J/cm2. It wasshown that the fusion of cells was completed within5-60 minutes depending on the cell type. Successfulpermeabilization of a cell membrane andoptoinjection of a membrane impermeable dye wasperformed with the help of a compact laser systemfor cell microsurgery DissCell-F (ytterbium laser,1050 nm, 75 MHz, ~115 fs). In both cases the laserirradiation parameters were thoroughly optimized toachieve high viability of treated cells and highefficiency of the procedures of cell fusion andoptical injection.
Acta Naturae | 2015
M. A. Filatov; Yu. V. Khramova; M. L. Semenova