Yuan Xianjun

Isolation and molecular identification of lactic acid bacteria from King grass and their application to improve the fermentation quality of sweet Sorghum

The aim of the present study was isolation and molecular identification of lactic acid bacteria from King grass and their application to improve the fermentation quality of sweet Sorghum. Seventy-six strains of LAB were isolated; five strains were selected for Physiological and morphological tests and 16Sxa0rRNA sequencing. All five strains grew at different pH 3.5–8.0, different temperature 35, 40, 45, 50xa0°C and different NaCl concentrations 3, 6.5, 9.5%. Strains HDASK were identified Lactobacillus plantarum and SK3907, SK2A32, SK3A42 and ASKDD Pediococcus acidilactici. Three isolated strains and one commercial strain were added to sweet sorghum. Silage was prepared of four treatments and one control with three replicates as control (SKC, adding 2xa0ml/kg sterilizing water), L. plantarum commercial bacteria (SKP), L. plantarum (HDASK) isolated from King grassxa0(SKA), P. acidilacticixa0(SK3907) isolated from King grassxa0(SKB) and P. acidilacticixa0(ASKDD) isolated from King grassxa0(SKD). All silage were prepared using polyethylene terephthalate bottles, and incubated at room temperature for different ensiling days. The level of pH, acetic acid, NH3-N, water soluble carbohydrate and butyric acid was significantly (Pu2009<u20090.05) decreased. Lactic acid, ethanol and propionic acid (PA) was significantly (Pu2009<u20090.05) increased in treatments compared to control. The dry matter, propionic acid neutral detergent fiber, acid detergent fiber did not significantly (Pu2009<u20090.05) differ among the treatments but the values were increased and decreased. The number of yeast, mold and LAB were significantly (Pu2009<u20090.05). It is suggested that the supplementation of LAB could enhanced the fermentation quality of sweet Sorghum silage.Graphical Abstract

Microbiological and chemical profiles of elephant grass inoculated with and without Lactobacillus plantarum and Pediococcus acidilactici

The study was conducted to evaluate the microbiological and chemical profiles of elephant grass inoculated with and without different wild strains of lactic acid bacteria. Silage was prepared of four treatments and one control with three replicates as control (EKC, adding 2xa0ml/kg sterilizing water), Lactobacillus plantarum (USA commercial bacteria) (EKP), Lactobacillus plantarum (EKA), Pediococcus acidilactici (EKB), and Pediococcus acidilactici (SKD) isolated from King grass. Silage were prepared using polyethylene terephthalate bottles, and incubated at room temperature for different ensiling days. The pH and acetic acid (AA) were significantly (Pu2009<u20090.05) reduced and lactic acid (LA), butyric acid (BA), and ethanol were significantly increased (Pu2009<u20090.05) at 3, 5, 7, and 14xa0days in treatment groups as compared to control. Water-soluble carbohydrate (WSC) and NH3–N concentration was not affected at days 3, 5, and 7, but significantly (Pu2009<u20090.05) reduced at 14xa0days in treatment groups as compared to control. The LA, BA, and ethanol were significantly (Pu2009<u20090.05) increased and AA, WSC NH3–N, and yeast were significantly (Pu2009<u20090.05) decreased at 30xa0days of ensiling in treatment groups as compared to control. It is recommended that the inoculation of LAB could improve the fermentation quality of elephant grass silage and further effort is needed to evaluate these effects on silage produced on farm scale and on animal production performance.

Careful Surgery, Management and Treatment of Feline (Cat) Breast Cancer

Objective: The aim of present study investigates the advanced breast cancer (malignant mammary tumors) surgical removal, effects of drugs and improving the quality of life. The terms of tumor and cancer are sometimes used interchangeably which can be misleading, there are two type of tumor, benign and malignant, but all tumors are not a cancerous. Methods: The study was conducted in Richmond Crawford Veterinary Hospital, Karachi, Pakistan (RCVH). A 13-year old female Household Pet Cat about 2 kg body weight was presented with the barest swelling complaint in a Richmond Crawford Veterinary Hospital, Karachi. There was partial reduction in swelling the right nipple, reaped growth of tumors, ulceration, oozing yellowish fluid. After the tentative clinical and laboratory diagnosis the excessive growth of tumor was removed surgically. Then we applied available medicines like injection Adriamycin 1mg/kg I/V, syp- cyclophosphamide 0.2mg/kg, and syp- vidayline 2 tea spoons morning and evening, every 24 hours for fifteen days. Results: And after the fifteen days the cat wound decreased gradually and successfully and improving the quality of life. Then again coming after six months for a checking there were no signs of breast cancer. Conclusions: For the better recovery of breast cancer, we recommended surgical intervention to remove the infected tissues and regularly washed with povidone and provide recommended medicine like injection Adriamycin 1mg/kg I/V, syp-cyclophosphamide 0.2mg/kg, and syp- vidayline 2 tea spoons every 24 hours for fifteen days.