Yuanbin Zhang

Sex-specific responses of Populus yunnanensis exposed to elevated CO2 and salinity

Populus yunnanensis Dode., a native dioecious woody plant in southwestern China, was employed as a model species to study sex-specific morphological, physiological and biochemical responses to elevated CO2 and salinity. To investigate the effects of elevated CO2 , salinity and their combination, the cuttings were exposed to two CO2 regimes (ambient CO2 and double ambient CO2 ) and two salt treatments in growth chambers. Males exhibited greater downregulation of net photosynthesis rate (Anet ) and carboxylation efficiency (CE) than females at elevated CO2 , whereas these sexual differences were lessened under salt stress. On the other hand, salinity induced a higher decrease in Anet and CE, more growth inhibition and leaf Cl(-) accumulation and more damage to cell organelles in females than in males, whereas the sexual differences in photosynthesis and growth were lessened at elevated CO2 . Moreover, elevated CO2 exacerbated membrane lipid peroxidation and organelle damage in females but not in males under salt stress. Our results indicated that: (1) females are more sensitive and suffer from greater negative effects than do males under salt stress, and elevated CO2 lessens the sexual differences in photosynthesis and growth under salt stress; (2) elevated CO2 tends to aggravate the negative effects of salinity in females; and (3) sex-specific reactions under the combination of elevated CO2 and salinity are distinct from single-stress responses. Therefore, these results provide evidence for different adaptive responses between plants of different sexes exposed to elevated CO2 and salinity.

Effects of elevated CO2 and temperature on photosynthesis and leaf traits of an understory dwarf bamboo in subalpine forest zone, China

The dwarf bamboo (Fargesia rufa Yi), growing understory in subalpine dark coniferous forest, is one of the main foods for giant panda, and it influences the regeneration of subalpine coniferous forests in southwestern China. To investigate the effects of elevated CO₂, temperature and their combination, the dwarf bamboo plantlets were exposed to two CO₂ regimes (ambient and double ambient CO₂ concentration) and two temperatures (ambient and +2.2°C) in growth chambers. Gas exchange, leaf traits and carbohydrates concentration were measured after the 150-day experiment. Elevated CO₂ significantly increased the net photosynthetic rate (Anet ), intrinsic water-use efficiency (WUEi ) and carbon isotope composition (δ¹³C) and decreased stomatal conductance (g(s)) and total chlorophyll concentration based on mass (Chl(m)) and area (Chl(a)). On the other hand, elevated CO₂ decreased specific leaf area (SLA), which was increased by elevated temperature. Elevated CO₂ also increased foliar carbon concentration based on mass (C(m)) and area (C(a)), nitrogen concentration based on area (N(a)), carbohydrates concentration (i.e. sucrose, sugar, starch and non-structural carbohydrates) and the slope of the A(net)-N(a) relationship. However, elevated temperature decreased C(m), C(a) and N(a). The combination of elevated CO₂ and temperature hardly affected SLA, C(m), C(a), N(m), N(a), Chl(m) and Chl(a). Variables Anet and Na had positive linear relationships in all treatments. Our results showed that photosynthetic acclimation did not occur in dwarf bamboo at elevated CO₂ and it could adjust physiology and morphology to enable the capture of more light, to increase WUE and improve nutritional conditions.

A new viewpoint to understand the response of leaf dark respiration to elevated CO2 concentration

By investigating the RD-Ca (dark respiration rate-atmospheric CO2 concentration) and PN (net photosynthetic rate)-Ca curves of bamboo (Fargesia denudata) and poplar (Populus cathayanna), we found that: (1) the minimal RD was close to ambient CO2 concentration, and the elevated or decreased atmospheric CO2 concentration enhanced the RD of both species; (2) the response curves of RD-Ca were simulated well by quadratic function. This phenomenon might be an inherent property of leaf RD of F. denudata and P. cathayanna. If this was true, it implies that effect of CO2 on RD could be interpreted with the relationship of RD-Ca curves and the quadratic function.

Growth and Wood/Bark Properties of Abies faxoniana Seedlings as Affected by Elevated CO2

Growth and wood and bark properties of Abies faxoniana seedlings after one years exposure to elevated CO2 concentration (ambient + 350 (+/- 25) micromol/mol) under two planting densities (28 or 84 plants/m(2)) were investigated in closed-top chambers. Tree height, stem diameter and cross-sectional area, and total biomass were enhanced under elevated CO2 concentration, and reduced under high planting density. Most traits of stem bark were improved under elevated CO2 concentration and reduced under high planting density. Stem wood production was significantly increased in volume under elevated CO2 concentration under both densities, and the stem wood density decreased under elevated CO2 concentration and increased under high planting density. These results suggest that the response of stem wood and bark to elevated CO2 concentration is density dependent. This may be of great importance in a future CO2 enriched world in natural forests where plant density varies considerably. The results also show that the bark/wood ratio in diameter, stem cross-sectional area and dry weight are not proportionally affected by elevated CO2 concentration under the two contrasting planting densities. This indicates that the response magnitude of stem bark and stem wood to elevated CO2 concentration are different but their response directions are the same.

Effects of exotic plantation forests on soil edaphon and organic matter fractions

There is uncertainty and limited knowledge regarding soil microbial properties and organic matter fractions of natural secondary forest accompanying chemical environmental changes of replacement by pure alien plantation forests in a hilly area of southwest of Sichuan province China. The aim of this study was to evaluate the impact of natural secondary forest (NSF) to pure Cryptomeria fortunei forest (CFF) and Cunninghamia lanceolata forest (CLF) on soil organic fractions and microbial communities. The results showed that the soil total phospholipid fatty acids (PLFAs), total bacteria and fungi, microbial carbon pool, organic recalcitrant carbon (C) and (N) fractions, soil microbial quotient and labile and recalcitrant C use efficiencies in each pure plantation were significantly decreased, but their microbial N pool, labile C and N pools, soil carbon dioxide efflux, soil respiratory quotient and recalcitrant N use efficiency were increased. An RDA analysis revealed that soil total PLFAs, total bacteria and fungi and total Gram-positive and Gram-negative bacteria were significantly associated with exchangeable Al3+, exchangeable acid, Al3+, available P and Mg2+ and pH, which resulted into microbial functional changes of soil labile and recalcitrant substrate use efficiencies. Modified microbial C- and N-use efficiency due to forest conversion ultimately meets those of rapidly growing trees in plantation forests. Enlarged soil labile fractions and soil respiratory quotients in plantation forests would be a potential positive effect for C source in the future forest management. Altogether, pure plantation practices could provoke regulatory networks and functions of soil microbes and enzyme activities, consequently leading to differentiated utilization of soil organic matter fractions accompanying the change in environmental factors.

Contrasting effects of warming on pioneer and fibrous roots growth in Abies faxoniana seedlings at low and high planting density

Key messageDistinct differences in pioneer and fibrous roots acclimation to climate warming.AbstractThis study was conducted to determine whether belowground parts of plants at different planting density differ in their responses to elevated temperature (ET). We investigated plant growth, pioneer and fibrous roots growth, root nonstructural carbohydrates, and root colonization of Abies faxoniana seedlings grown in environment-controlled chambers with two different planting densities. Warming has more pronounced positive effects at low density. Although ET did not affect total root biomass, fibrous roots biomass increased under ET at low planting density while pioneer roots biomass was unaffected by ET, indicating that this species may maintain the main framework of the root system with a high capability for water and N absorption under ET. ET increased root nonstructural carbohydrates concentration and ectomycorrhiza colonization in fibrous roots. Increased root nonstructural carbohydrates in response to ET might be associated with the increased roots ectomycorrhizal infection under ET. The present study provided experimental evidence of distinct differences in pioneer and fibrous roots acclimation to climate change.