Yuanyuan Jia

PREPARATION AND APPLICATION OF BACTERIAL CELLULOSE SPHERE: A NOVEL BIOMATERIAL

ABSTRACT This paper explores preparation of spherical bacterial cellulose (BC), a novel biomaterial, and its adsorption capacity is charaterized. The effect of shaking speed and culture duration on fermentation production of BC spheres is analyzed; BC spheres are produced after 72 h fermention at 30°C with a shaking speed of 160 rpm. The spheres have a diameter range of 3–5 mm. The scanning electron micrograph photograph shows that BC spheres have a loose and porous structure. Repetition using tests on adsorption of Bovine serum albumin (BSA) and Pb2+ had been carried out. The results indicated that BC spheres can be recovered from BSA-BC complex and Pb-BC complex by eluting with NaOH and sodium citrate solution, respectively. So BC spheres have a vast potential for application in the fields of biomaterial bioseparation and sewage treatment.

Preparation and Characterization of Bacterial Cellulose Tube

Fermentation technique A (using oxygen-permeable tube as scaffold in fermentation vessel) and fermentation technique B (using oxygen-permeable tube as scaffold, also as fermentation vessel) were applied in preparation of bacterial cellulose (BC) tubes. Several types of oxygen-permeable tubes were tested in investigating their effects on biosynthesis of BC tubes by Gluconacetobacter xylinus. The results showed that tubular bacterial cellulose with uniform thickness when silicone tube was served as a scaffold. The BC production were significantly higher in technique B process than technique A. Tensile strengths of BC tubes were also measured, 0.35 MPa from technique A process, 50.25 MPa from technique B process. Scanning electron microscopy (SEM) revealed the BC structure of technique B tubes was more homogeneous and denser than that of technique A tubes. The BC tubes manufactured with silicone as oxygen-permeable scaffold according to fermentation technique B resulted in desired mechanical properties and were expected to be used as a scaffold for tissue engineered blood vessels and cellulose sausage casings.

Performance Improvement for Biomedical Material - Bacterial Cellulose

Bacterial cellulose has been deemed as a promising biomedical and tissue engineering material, due to the excellent biocompatibility, bio-degradability, water holding capacity and mechanical properties it possesses. This paper aims to improve its properties to meet the requirements of biomedical materials. The reasonable way is to impregnate BC pellicle with epsiv-PL solution and then immerse it into glycerol solution. Water absorbance, softness, flexibility, tensile strength and anti-bacterial activity have been improved successfully by aforesaid treatment.